Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,541,039 Δ1 bp 100% intergenic (‑54/+262) PP_4872 ← / ← dnaB hypothetical protein/replicative DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,541,0390G.100.0% 139.5 / NA 31intergenic (‑54/+262)PP_4872/dnaBhypothetical protein/replicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (17/14);  total (17/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATAGTCGTAGAGTGGTTTGGCTGCTTGCATGTCAGTGACCGGTTCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTTGGGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCATCGCGACGCAAGGCCGCTCCCACATTTGTTGCAACGTGC  >  NC_002947/5540902‑5541181
                                                                                                                                         |                                                                                                                                              
aTAGTCGTAGAGTGGTTTGGCTGCTTGCATGTCAGTGACCGGTTCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTTgggggggcgt                                                                                                                                       >  1:88572/1‑144 (MQ=255)
   gTCGTAGAGTGGTTTGGCTGCTTGCATGTCAGTGACCGGTTCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAgg                                                                                                                                  <  5:156906/148‑1 (MQ=255)
            tGGTTTGGCTGCTTGCATGTCAGTGACCGGTTCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGttggttt                                                                                                                        <  4:173374/149‑1 (MQ=255)
                    ctgctTGCATGTCAGTGACCGGTTCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTTggg                                                                                                                                              <  6:234088/120‑1 (MQ=255)
                    ctgctTGCATGTCAGTGACCGGTTCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTTggg                                                                                                                                              >  5:234088/1‑120 (MQ=255)
                          gCATGTTAGTGACCGGTTCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGAGTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGt                                                                                                            >  2:72555/1‑147 (MQ=255)
                            aTGTCATTGACCGGTTCTGGTGTGCAGTGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTGGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAgg                                                                                                        <  5:256470/149‑1 (MQ=255)
                             tGTCAGTGACCGGTTCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAggtg                                                                                                                                >  6:276455/1‑124 (MQ=255)
                             tGTCAGTGACCGGTTCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAggtg                                                                                                                                <  5:276455/124‑1 (MQ=255)
                               tCACTGCCCGGGTCGGGTGGGCAGGGAAAACCCGGGCGCGGAAAAAAGCACAAATTTTGACCAAATCCCACTGGATTCGGGGGGATTGGGGACGAGAGCGACCCGT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGgc                                                                                                      <  1:144874/148‑1 (MQ=255)
                                       cGGTTCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGgtg                                                                                              >  5:262290/1‑148 (MQ=255)
                                          ttCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAg                                                                                                                                   <  6:276645/108‑1 (MQ=255)
                                          ttCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAg                                                                                                                                   >  5:276645/1‑108 (MQ=255)
                                               ggttgCACGGAAATCTCGGGCGCGGAATATATCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGTTTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGac                                                                                        <  7:238620/143‑1 (MQ=255)
                                                    cAGGGAAATCGCGGGCGCGGAATATAGCACAAATATTGACCAAATCCGACTCGATTCGTGGGGATTGTTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTAGGTTTGGTGGGCTTCGTGAGGGCGCCAGGTGTACGACACAGCAGt                                                                                >  4:54193/1‑149 (MQ=255)
                                                      gggAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGttt                                                                              <  2:141791/149‑1 (MQ=255)
                                                                    gcgGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGaaa                                                                <  6:262290/149‑1 (MQ=255)
                                                                                acaAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGtt                                                                               <  4:41052/122‑1 (MQ=255)
                                                                                acaAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGtt                                                                               >  3:41052/1‑122 (MQ=255)
                                                                                acaAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCgccgcc                                                    >  4:17765/1‑149 (MQ=255)
                                                                                acaAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTCAGGAGAGCGACAGAT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGGTTGGAGAGGGCGCCACGTGTACGACACAGCAGTTTCAGCGCCTGGGAAATCGAGCgccgcc                                                    >  4:196000/1‑149 (MQ=255)
                                                                                            cAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCAt                                        >  7:106954/1‑149 (MQ=255)
                                                                                               aTCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCATcgc                                     <  2:88572/149‑1 (MQ=255)
                                                                                                  cGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCATCGCGAc                                  >  5:39846/1‑149 (MQ=255)
                                                                                                   gACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGAGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTc                                                                             >  7:284402/1‑105 (MQ=255)
                                                                                                                       gTGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCATCGCGACGCAAGGCCGCTCCAACATttg             >  1:296212/1‑149 (MQ=255)
                                                                                                                        tGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCATCGCGACGCAAGGCCGCTCCCACATttgt            >  7:30216/1‑149 (MQ=255)
                                                                                                                        tGAGGAGAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCATCGCGACGCAAGGCCGCTCCCACATttgt            >  8:10143/1‑149 (MQ=255)
                                                                                                                         gaggagAGCGACAGTT‑GGGGGGGCTTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACATCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGGGCATCGCGACGCAAGGCCGCTCCCACATttgtt           <  3:54193/149‑1 (MQ=255)
                                                                                                                         gaggagAGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCATCGCGACGCAAGGCCGCTCCCACATttgtt           <  3:17765/149‑1 (MQ=255)
                                                                                                                            gagaGCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGAGCCTGTCAAATCGAGCGCCGCCCGCGCGGCGCATCGCGACGCAAGGCCGCTCCCACATTTGTTGc         >  6:298577/1‑148 (MQ=255)
                                                                                                                              gagCGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCATCGCGACGCAAGGCCGCTCCCACATTTGTTGCAAc      >  4:10356/1‑149 (MQ=255)
                                                                                                                                 cGACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCATCGCGACGCAAGGCCGCTCCCACATTTGTTGCAACGTg   <  6:39846/149‑1 (MQ=255)
                                                                                                                                  gACAGTT‑GGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCATCGCGACGCAAGGCCGCTCCCACATTTGTTGCAACGTGc  >  2:229512/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                              
ATAGTCGTAGAGTGGTTTGGCTGCTTGCATGTCAGTGACCGGTTCTGGTGTGCAGGGAAATCGCGGGCGCGGAATATAGCACAAATTTTGACCAAATCCGACTGGATTCGTGGGGATTGGTGAGGAGAGCGACAGTTGGGGGGGGCGTCAGGTGGTTGGTTTGGTGGGCTTGGTGAGGGCGCCAGGTGTACGACACAGCAGTTTCAGCGCCTGTGAAATCGAGCGCCGCCCGCGCGGCGCATCGCGACGCAAGGCCGCTCCCACATTTGTTGCAACGTGC  >  NC_002947/5540902‑5541181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑