| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,196,069:1 | +C | 7.3% | coding (446/690 nt) | ybgJ ← | allophanate hydrolase subunit 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,196,069 | 1 | . | C | 7.3% | 211.9 / 8.5 | 55 | coding (446/690 nt) | ybgJ | allophanate hydrolase subunit 1 |
| Reads supporting (aligned to +/- strand): ref base . (27/24); new base C (2/2); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTGCGGGCTGCCAGCACAGGCAG > NC_002947/5195925‑5196211 | tcacgctcacgGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAg‑g < 8:84288/146‑1 (MQ=255)tcacgctcacgGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAg‑g > 7:84288/1‑146 (MQ=255)tcacgctcacgGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCt > 7:48065/1‑149 (MQ=255)tcacgctcacgGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCt > 5:11179/1‑149 (MQ=255) cacgctcacgGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTACCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCCCGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGGCGAG‑GGCTg > 1:233446/1‑149 (MQ=255) aacaacCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGTGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCtcgtc < 1:291226/149‑1 (MQ=255) aacCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGgc < 8:148074/139‑1 (MQ=255) aacCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGgc > 7:148074/1‑139 (MQ=255) ccGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAGCGCCTGGCCAgccctcgt > 1:248781/1‑136 (MQ=255) ccGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAGCGCCTGGCCAgccctcgt > 1:244620/1‑136 (MQ=255) ccGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAGCGCCTGGCCAgccctcgt < 2:244620/143‑8 (MQ=255) ccGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAGCGCCTGGCCAgccctcgt < 2:248781/143‑8 (MQ=255) cACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTg < 4:278865/127‑1 (MQ=255) cACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTg > 3:278865/1‑127 (MQ=255) aCCGGGGTGCGCCCGCTCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCGGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGcc < 2:632/149‑1 (MQ=255) cgcCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGcc < 8:173594/149‑1 (MQ=255) cGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGaaa < 3:163155/149‑1 (MQ=255) cAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGa > 5:156936/1‑125 (MQ=255) cAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGa < 6:156936/125‑1 (MQ=255) ggTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCtcgtc > 3:27430/1‑121 (MQ=255) ggTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCtcgtc < 4:27430/121‑1 (MQ=255) ggTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGt > 5:303176/1‑149 (MQ=255) ttCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGc < 4:171550/149‑1 (MQ=255) ttCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGc > 4:272979/1‑149 (MQ=255) ccGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAgg > 5:156224/1‑149 (MQ=255) ggCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGc < 6:113141/149‑1 (MQ=255) gATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCCCGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCaaa < 2:233446/149‑1 (MQ=255) aTACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAac < 2:117110/149‑1 (MQ=255) ccGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCgg < 6:156224/148‑1 (MQ=255) gggTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTa < 8:48065/149‑1 (MQ=255) tAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGc > 3:33479/1‑109 (MQ=255) tAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGc < 4:33479/109‑1 (MQ=255) gccgccGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAac < 8:90980/135‑1 (MQ=255) gccgccGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAac > 7:90980/1‑135 (MQ=255) cgccgTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACCCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGt > 1:230596/1‑148 (MQ=255) gTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCAcc < 3:272979/149‑1 (MQ=255) tCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGc > 1:243096/1‑148 (MQ=255) ggCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGaa > 1:159461/1‑149 (MQ=255) aCCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAAtct > 8:10060/1‑149 (MQ=255) ccACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCggg > 7:278447/1‑116 (MQ=255) ccACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCggg < 8:278447/116‑1 (MQ=255) ccACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTcc > 3:304125/1‑149 (MQ=255) ccACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTcc > 5:235591/1‑149 (MQ=255) aCGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCt > 4:290851/1‑149 (MQ=255) aCGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCt < 5:140667/149‑1 (MQ=255) cgcgCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTg > 7:216982/1‑149 (MQ=255) cgcgCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTg < 6:11179/149‑1 (MQ=255) gcTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACACAAGCCGCTGCgg < 4:304125/149‑1 (MQ=255) ggggTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTGCGGGCTGCCAg < 2:243096/149‑1 (MQ=255) ggggTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTGCGGGCTGCCAg > 2:100550/1‑149 (MQ=255) gggTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTGCGGGCTGCCAGc < 2:159461/149‑1 (MQ=255) cTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTGCGGGCTGCCAGCACAgg > 8:138762/1‑149 (MQ=255) tCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTGCGGGCTGCCAGCACAgg > 3:217903/1‑148 (MQ=255) tCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTGCGGGCTGCCAGCACAgg > 3:195730/1‑148 (MQ=255) tCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTGCGGGCTGCCAGCACAgg > 5:305761/1‑148 (MQ=255) aGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTGCGGGCTGCCAGCACAGGCa < 6:303176/148‑1 (MQ=255) aGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTGCGGGCTGCCAGCACAGGCAg > 6:12442/1‑149 (MQ=255) g‑ggCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCg > 8:187265/1‑120 (MQ=255) g‑ggCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCg < 7:187265/120‑1 (MQ=255) | TCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACACCGGGTAATCGCGTTCACCGTGCAAGCGAACAATCTCCGCTTCACTCAAGCCGCTGCGGGCTGCCAGCACAGGCAG > NC_002947/5195925‑5196211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |