Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,277,005:1 +C 100% intergenic (‑309/‑35) cioA ← / → PP_4652 cyanide insensitive ubiquinol oxidase subunit I/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,277,0051.C97.7% 146.1 / ‑3.5 44intergenic (‑309/‑35)cioA/PP_4652cyanide insensitive ubiquinol oxidase subunit I/membrane protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (24/19);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

GAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCG‑‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGATGCTCACCGTGGCCATC  >  NC_002947/5276857‑5277147
                                                                                                                                                     |                                                                                                                                                
gaatgCTAGGAAGCATAGTCCCGAATGGCCCAATGGGCCGTGTGGTGGATTACCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGAAAAAAGTAACCGCTTGTTACAAAAAGATGATACGCTgcg                                                                                                                                                   >  8:204967/1‑149 (MQ=255)
 aatgCTAGGAAGCATAGTCCAGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCCCTTGTAACAAACAGATGATACGCTgcg‑‑c                                                                                                                                                >  8:70522/1‑149 (MQ=255)
                  tCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGcc                                                                                                                               >  1:302528/1‑149 (MQ=255)
                         tGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGGTACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGccc                                                                                                                        <  7:204967/149‑1 (MQ=255)
                                    gCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGa                                                                                                             <  8:171941/149‑1 (MQ=255)
                                     ccGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGAt                                                                                                            <  6:178993/149‑1 (MQ=255)
                                        tgtgGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTc                                                                                                         <  2:302528/149‑1 (MQ=255)
                                          tggtggATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTcc                                                                                                        <  6:257684/148‑1 (MQ=255)
                                                            ccTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGCGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAgttgt                                                                                     >  8:232296/1‑149 (MQ=255)
                                                             cTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAgttgtt                                                                                    >  6:104188/1‑149 (MQ=255)
                                                                   ccTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGAtt                                                                                                    >  8:233867/1‑127 (MQ=255)
                                                                   ccTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGAtt                                                                                                    <  7:233867/127‑1 (MQ=255)
                                                                     tCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGc                                                                                  <  8:15307/144‑1 (MQ=255)
                                                                     tCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTACGCACCGCAGttgttgggagat                                                                             >  7:15307/1‑143 (MQ=255)
                                                                     tCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTccgcaccgca                                                                                          >  5:88659/1‑135 (MQ=255)
                                                                     tCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTccgcaccgca                                                                                          <  6:88659/135‑1 (MQ=255)
                                                                         ccTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAgttgtt                                                                                    >  8:141170/1‑137 (MQ=255)
                                                                         ccTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAgttgtt                                                                                    <  7:141170/137‑1 (MQ=255)
                                                                         ccTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCg                                                                        >  2:37990/1‑149 (MQ=255)
                                                                           tctTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAgttgt                                                                                     >  8:30389/1‑134 (MQ=255)
                                                                           tctTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAgttgt                                                                                     <  7:30389/134‑1 (MQ=255)
                                                                               tGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCttt                                                                  >  5:36968/1‑149 (MQ=255)
                                                                                 aTTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCG‑‑CCCACCCTCTACTGCGCCGAGGCCACTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTc                                                                >  8:233480/1‑149 (MQ=255)
                                                                                  ttAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCt                                                               <  1:37990/149‑1 (MQ=255)
                                                                                         tGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTc                                                        >  2:215517/1‑149 (MQ=255)
                                                                                             aaaGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTctggc                                                    <  1:215517/149‑1 (MQ=255)
                                                                                             aaaGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTctggc                                                    <  5:104188/149‑1 (MQ=255)
                                                                                                              aaaCAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCAcc                                   >  6:54480/1‑149 (MQ=255)
                                                                                                               aaCAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCg                                  >  2:213577/1‑149 (MQ=255)
                                                                                                                aCAGTAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGc                                                            <  6:232388/122‑1 (MQ=255)
                                                                                                                    tAACCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGc                             >  4:289255/1‑149 (MQ=255)
                                                                                                                      aCCGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCCCAGATGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCgg                           >  1:220489/1‑149 (MQ=255)
                                                                                                                       ccGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGc                          <  1:148568/149‑1 (MQ=255)
                                                                                                                       ccGCTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGc                          <  1:143589/149‑1 (MQ=255)
                                                                                                                          cTTGTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTc                       <  7:70522/149‑1 (MQ=255)
                                                                                                                             gTTACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTcc                      <  5:54480/147‑1 (MQ=255)
                                                                                                                              ttACAAACAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTc                       >  5:146461/1‑145 (MQ=255)
                                                                                                                                  aaaCAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGATGCt               >  8:209772/1‑149 (MQ=255)
                                                                                                                                  aaaCAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGATGCt               >  2:199249/1‑149 (MQ=255)
                                                                                                                                    aCAGATGATACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGATGCTCa             >  8:96788/1‑149 (MQ=255)
                                                                                                                                    aCAGATGATACGCTGCGC‑CCC‑CCCTCGACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGATGCTCa             >  1:9995/1‑149 (MQ=255)
                                                                                                                                    aCAGATGATACGCTGCGC‑CCC‑CCCTCGACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGATGCTCa             >  1:4653/1‑149 (MQ=255)
                                                                                                                                       gatgatACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCa                               <  7:86397/128‑1 (MQ=255)
                                                                                                                                       gatgatACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCa                               >  8:86397/1‑128 (MQ=255)
                                                                                                                                            tACGCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGATGCTCACCGTGGc      >  4:175941/1‑148 (MQ=255)
                                                                                                                                               gCTGCGC‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGATGCTCACCGTGGCCATc  >  5:52542/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCG‑‑CCC‑CCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGATGCTCACCGTGGCCATC  >  NC_002947/5276857‑5277147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑