| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 747,612:1 | +G | 100% | intergenic (‑739/‑55) | PP_0638 ← / → PP_5448 | transposase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 747,612 | 1 | . | G | 92.3% | 35.7 / ‑2.6 | 13 | intergenic (‑739/‑55) | PP_0638/PP_5448 | transposase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (12/0); total (13/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
ATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGT‑GGGG‑GGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGATC > NC_002947/747468‑747749 | atcatcGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGT‑gggg < 4:280898/149‑1 (MQ=255) atcGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGATCGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGTCTCTCACTGGCCGACACCACTCTGGGTCATCTTCGTGTTGTGTGTTGT‑gggg‑gg < 6:326543/148‑1 (MQ=255) ttAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGT‑GGGGTGGGgcgc > 3:185918/1‑149 (MQ=255) ccACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGT‑gggg‑gggg < 3:250501/136‑1 (MQ=255) ccACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGT‑gggg‑gggg > 4:250501/1‑136 (MQ=255) gaTCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGc > 1:930/1‑149 (MQ=255) gttgtGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTAGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATgc > 3:274163/1‑149 (MQ=255) ttgtGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTGGGTGAATGGGTGGAGCGGAGGGGGCGCAACCAATGATgcg > 3:184318/1‑149 (MQ=255) ttGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCaa > 7:22519/1‑148 (MQ=255) gggCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTGGGTGAATGGCTGGGGCGGTGGGGGCGCAATCAATGTTGCGCGCCAAtg > 5:195409/1‑147 (MQ=255) gggCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCg > 7:37584/1‑117 (MQ=255) gCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATc > 3:40646/1‑149 (MQ=255) ttCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGAc > 3:231977/1‑135 (MQ=255) ttCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGt > 1:253472/1‑149 (MQ=255) gTCATCGTCGTGTTGTGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTTGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGTTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCaatatc > 1:226381/1‑144 (MQ=255) tcgtGTTGTGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTgcg > 3:8043/1‑149 (MQ=255) ttgtGGGTGGTGGGGG‑GGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCTATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGATc > 5:233746/1‑149 (MQ=255) | ATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGT‑GGGG‑GGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGATC > NC_002947/747468‑747749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |