| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,275,098:1 | +G | 100% | intergenic (‑295/+17) | PP_5462 ← / ← PP_5463 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,275,098 | 1 | . | G | 87.5% | 38.4 / 1.2 | 16 | intergenic (‑295/+17) | PP_5462/PP_5463 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (10/4); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
ATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGTTCGTCTGCG > NC_002947/1274951‑1275238 | aTTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGTCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑g > 3:224562/1‑149 (MQ=255) ccgctctCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATACGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATTAGCAAAGCCCGCCGATGGGCGGGCTTCA‑g < 7:276318/142‑1 (MQ=255) tATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTACAATAAGGTGCGGGATAGCGGGAATGATGGGGGTGATTGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGACGAATGCCGGGCTTCA‑ggagaacgt > 8:276318/1‑141 (MQ=255) gagaGTGAATGTCGAAGCCTGCTTCGCGGTTACGTCGGCTGCAATAAGGTGCGGGAGGGCGGTATTGATGGTGGGGATGGTGGTTTAGGTGAAAGGTGATTGAGCAAAGCCCGCCGATTGGCGGGCTTCAGGGGGGGGGCAGAAGGGCt > 2:206688/1‑149 (MQ=255) aTGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTTATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGtacta > 5:6972/1‑149 (MQ=255) ttCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCg > 1:232582/1‑149 (MQ=255) gTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCAcccgc > 1:4030/1‑149 (MQ=255) cGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGGCAGAAGGGCTATCGtact > 1:219432/1‑98 (MQ=255) atcttgGCGGTGCTGGTGGTAAAGGGGAAACGCCACTCAGCACAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAg < 6:6972/144‑1 (MQ=255) aTGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACttt < 5:298567/149‑1 (MQ=255) aTGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACtt > 6:298567/1‑148 (MQ=255) gtggtAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGATTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAg < 4:224562/149‑1 (MQ=255) aaGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACt > 7:97104/1‑149 (MQ=255) aaaGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCttt > 5:57922/1‑149 (MQ=255) aaaGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCtt < 8:97104/148‑1 (MQ=255) ggAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGa > 6:18543/1‑149 (MQ=255) cccGCCGATGGGCGGGCTTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGc < 6:57922/149‑1 (MQ=255) ggcgggcTTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTCTTCGTc > 7:174495/1‑148 (MQ=255) gcTTCAGGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGTTCGTCTGCg > 6:150099/1‑147 (MQ=255) | ATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGTTCGTCTGCG > NC_002947/1274951‑1275238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |