| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,192,436:1 | +C | 100% | intergenic (+45/‑159) | PP_1937 → / → PP_1938 | helicase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,192,436 | 1 | . | C | 91.2% | 102.1 / 3.9 | 34 | intergenic (+45/‑159) | PP_1937/PP_1938 | helicase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (14/17); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GATGTTATTCTGAAGAAAATCCAAGGCGTTGACCTTCGACGGCGTTGGGAAGAGCGAATCGTTGCTTGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCG‑C‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACGGGACACTCGGGGAAAGCCTTACACTGCCGCTAAGAATGCCAGCTC > NC_002947/2192290‑2192579 | gATGTTATTCTGAAGAAAATCCAAGGCGTTGACCTTCGACGGCGTTGGGAAGAGCGAATCGTTGCTTGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATgccg‑c‑c > 1:159323/1‑149 (MQ=255) tGAAGAAAATCCAAGGCGTTGACCTTCGACGGCGTTGGGAAGAGCGAATCGTTGCTTGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGt > 7:6745/1‑149 (MQ=255) gaaAATCCAAGGCGTTGACCTTCGACGGCGTTGGGAAGAGCGAATCGTTGCTTGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGttttt > 4:221575/1‑149 (MQ=255) aaaTCCAAGGCGTTGACCTTCGACGGCGTTGGGAAGAGCGAATCGTTGCTTGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTg < 2:159323/149‑1 (MQ=255) ttGACCTTCGACGGCGTTGGGAAGAGCGAATCGTTGCTTGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGtttt < 8:6745/149‑1 (MQ=255) cTTCGACGGCGTTGGGAAGAGCGAATCGTTGCTTGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTg < 5:211656/135‑1 (MQ=255) cTTCGACGGCGTTGGGAAGAGCGAATCGTTGCTTGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTg > 6:211656/1‑135 (MQ=255) cGACGGCGTTGGGAAGAGCGAAGCGTTGCTTGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAgtgt < 7:244249/147‑1 (MQ=255) gACGGCGTTGGGAAGAGCGAATCGTTGCTTGTATTCTTGGGTATTGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCAAGGTCAAACCGCTACCTTTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGTGGATACGTTTTttgtttgt > 1:68666/1‑149 (MQ=255) aaGAGCGAATCGTTGCTTGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATAGCAAGCTGc < 8:126369/149‑1 (MQ=255) ttGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTTGTGATACGGTTTTTGTGTGTTATTGCAAGCTGCCGTTGGGGCTGAGCAc > 4:12114/1‑149 (MQ=255) ttGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATg < 7:285343/124‑1 (MQ=255) ttGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATg > 8:285343/1‑124 (MQ=255) tGTATTCTTGGGTA‑AGGGCAAAGCGCCGAGGAGTGATGGATCTCTACCCAGGTCCAACCGCTACATGTCATGTGAATGCCG‑CACCCCCGCCGATTATTGGTTAATCGTTTATAGTGGGATATGGCAAGCTGCCCTAGGGGCtacacac > 2:241326/1‑142 (MQ=255) tCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCG‑C‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGc < 3:291449/119‑1 (MQ=255) tCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCG‑C‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGc > 4:291449/1‑119 (MQ=255) gggCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTGTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTGGGGCTGAGCCCGTACGGCCCGGTCGtt > 8:268707/1‑149 (MQ=255) aaaGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGGTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTg < 2:68666/149‑1 (MQ=255) aaGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTgg > 1:183493/1‑149 (MQ=255) aGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTggg > 5:127217/1‑149 (MQ=255) cGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATaga < 4:140298/149‑1 (MQ=255) agTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTTGTGATACGGTTTTAGTTTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGGACGGCCCGCTACTTCCTGGGATAGAGccac > 8:287732/1‑146 (MQ=255) agTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACg < 3:12114/149‑1 (MQ=255) gTGAGGGAGCTGTACCCAGGTCCAACCGCGACCTGTAATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGAGACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACgg < 4:185804/149‑1 (MQ=255) tACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACGGGACACTCg < 6:127217/145‑1 (MQ=255) cAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACGGGACACTCGGGGAAAGc < 2:183493/149‑1 (MQ=255) cAGGTCCAACCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACGGGACACTCGGGGAAAGc < 3:221575/149‑1 (MQ=255) aaCCGCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACGGGACACTCGGGGAAAGCCTTacac > 4:202778/1‑149 (MQ=255) gCTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTTGTGATTCGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGGACGGCCCCCTGGTTCCGGGGATAGAGCACGGGACACTCGGGGAAAGCCTTAAACAGcc > 6:212840/1‑149 (MQ=255) cTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTggg < 6:264492/110‑1 (MQ=255) cTACCTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTggg > 5:264492/1‑110 (MQ=255) ccTGTCATGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACGGGACACTCGGGGAAAGCCTTACACTGCCGCt < 5:212840/148‑1 (MQ=255) aTGTGAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACGGGACACTCGGGGAAAGCCTTACACTGCCGCTAAGAATg > 6:298296/1‑149 (MQ=255) tgtgAATGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACGGGACACTCGGGGAAAGCCTTACACTGCCGCTAAGAATGc < 7:268707/149‑1 (MQ=255) tGCCGCC‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACGGGACACTCGGGGAAAGCCTTACACTGCCGCTAAGAATGCCAGCTc < 5:298296/149‑1 (MQ=255) | GATGTTATTCTGAAGAAAATCCAAGGCGTTGACCTTCGACGGCGTTGGGAAGAGCGAATCGTTGCTTGTATTCTTGGGTA‑TGGGCAAAGCGCCGAGGAGTGATGGATCTGTACCCAGGTCCAACCGCTACCTGTCATGTGAATGCCG‑C‑CCCCCGGCGTTTTTTGGTGATACGTTTTTAGTGTGTTATGGCAAGCTGCCGTTCGGGCTGAGCACGTACGGCCCGCTCGTTCCTGGGATAGAGCACGGGACACTCGGGGAAAGCCTTACACTGCCGCTAAGAATGCCAGCTC > NC_002947/2192290‑2192579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |