Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,735,143:1 +T 8.9% coding (40/3093 nt) PP_3302 → RND family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,735,1431.T 8.9% 164.3 / 12.9 45coding (40/3093 nt)PP_3302RND family transporter
Reads supporting (aligned to +/- strand):  ref base . (16/25);  new base T (2/2);  total (18/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

GTGACCTGCACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGGCGCCAC  >  NC_002947/3734995‑3735282
                                                                                                                                                     |                                                                                                                                           
gTGACCTGCACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAc                                                                                                                                              >  6:32861/1‑149 (MQ=255)
 tGACCTGCACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAc‑g                                                                                                                                            >  1:262013/1‑149 (MQ=255)
    ccTGCACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑gcga                                                                                                                                         <  4:89345/149‑1 (MQ=255)
         aCGCTGGCGATCGGATCGTGGCACTGGGTGCGCAGCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGat                                                                                                                                    <  5:272967/149‑1 (MQ=255)
          cGCTGGCGATCGGATCGTGGCACAGGGTGCGCATCTGCAGCGCGATGGAGTCGAGGTCATGGAACAGGATGAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGATGCGTTTCAACATATCCGCGCTCGCTGTAC‑GCGAGCTat                                                                                                                                    >  4:95466/1‑148 (MQ=255)
                           tGGCTCTGGGTTCGTATTTGCTGCGCGATGGCTTCGAGGTCATGGAACAGTCTCAAACAGCTTATGTCATGGGGCACCAACCATGATCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCTATCATTCACCCTCTTCCTGa                                                                                                                  <  8:126897/149‑1 (MQ=255)
                           tGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGa                                                                                                                  <  5:32861/149‑1 (MQ=255)
                           tGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGa                                                                                                                  >  6:185497/1‑149 (MQ=255)
                               aCTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCATTCACCCTCTTCCTGATtgt                                                                                                              >  7:59860/1‑149 (MQ=255)
                               aCTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATtgt                                                                                                              <  4:207381/149‑1 (MQ=255)
                                cTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATtgtg                                                                                                             <  1:54686/149‑1 (MQ=255)
                                          aTCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCt                                                                                                   >  7:42955/1‑149 (MQ=255)
                                          aTCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCt                                                                                                   >  7:37858/1‑149 (MQ=255)
                                          aTCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGC‑GTACTgcgagcg                                                                                                                                      <  4:96858/114‑1 (MQ=255)
                                          aTCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGC‑GTACTgcgagcg                                                                                                                                      >  3:96858/1‑114 (MQ=255)
                                               ctgcGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTc                                                                                                 <  4:1884/146‑1 (MQ=255)
                                                tgcGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGatc                                                                                                                                   <  6:645/111‑1 (MQ=255)
                                                  cgcgATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCg                                                                                             <  8:37858/147‑1 (MQ=255)
                                                  cgcgATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCg                                                                                             <  8:42955/147‑1 (MQ=255)
                                                            tCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCt                                                                                 >  2:75016/1‑149 (MQ=255)
                                                                ggTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCttttt                                                                             <  8:203432/149‑1 (MQ=255)
                                                                  tCATGGAACAGGCTCAAACAGCTCAGGTCATGGTGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTc                                                                            <  3:95466/148‑1 (MQ=255)
                                                                    aTGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCaa                                                                          <  1:59494/148‑1 (MQ=255)
                                                                        aaCAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGCGCCAGCTGCGTTTCAACTAATCCGCCCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCATGCTTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTgg                                                                     >  5:105203/1‑149 (MQ=255)
                                                                          cAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGc                                                                   >  8:745/1‑149 (MQ=255)
                                                                           aGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCATTCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGcc                                                                  <  8:59860/149‑1 (MQ=255)
                                                                           aGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGcc                                                                  <  5:185497/149‑1 (MQ=255)
                                                                            ggCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCg                                                                 >  2:191633/1‑149 (MQ=255)
                                                                             gCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGt                                                                >  7:184658/1‑149 (MQ=255)
                                                                             gCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGt                                                                >  4:20200/1‑149 (MQ=255)
                                                                              cTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTg                                                               <  8:148927/149‑1 (MQ=255)
                                                                                 aaaCAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGc                                                              <  2:262013/147‑1 (MQ=255)
                                                                                       cTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATc                                                      <  4:257874/149‑1 (MQ=255)
                                                                                        tCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATcc                                                     >  6:249809/1‑149 (MQ=255)
                                                                                                    gCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGt                                         <  1:191633/149‑1 (MQ=255)
                                                                                                      accaaccaTGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTg                                                               >  1:285182/1‑125 (MQ=255)
                                                                                                      accaaccaTGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTg                                                               <  2:285182/125‑1 (MQ=255)
                                                                                                          accaTGAGCCTGCTGCGTTTCAACTTATCCGCGCTCGCTGTAC‑GCGAGCGATCAATCACGCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGGTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCa                                   <  6:105203/149‑1 (MQ=255)
                                                                                                                  ccAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGa                           <  3:20200/149‑1 (MQ=255)
                                                                                                                       tGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTa                        >  2:293824/1‑147 (MQ=255)
                                                                                                                       tGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTa                        <  1:293824/147‑1 (MQ=255)
                                                                                                                         cGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCAc                    <  7:745/149‑1 (MQ=255)
                                                                                                                          gTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACt                   >  2:152322/1‑149 (MQ=255)
                                                                                                                           tttCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTg                  >  2:169365/1‑149 (MQ=255)
                                                                                                                                      gatcgcTCGC‑GTACTGCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCa                     <  3:256350/132‑1 (MQ=255)
                                                                                                                                      gatcgcTCGC‑GTACTGCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCa                     >  4:256350/4‑135 (MQ=255)
                                                                                                                                          gcTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGGCGCCa   <  1:75016/149‑1 (MQ=255)
                                                                                                                                           cTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGGCGCCAc  <  5:249809/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                           
GTGACCTGCACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGGCGCCAC  >  NC_002947/3734995‑3735282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑