| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,336,532:1 | +C | 100% | coding (683/885 nt) | PP_3811 ← | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,336,532 | 1 | . | C | 93.9% | 47.0 / ‑4.8 | 16 | A228G (GCT→GGT) | PP_3811 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (5/10); minor base A (1/0); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCACCTGCCGGTGCTGCGGATACACCGCCGACAGCTGCAGCGCGGGCGGGCGCCACTCATCCAGAACCGTCACCAGTTGCCCCTCCGCCAACGCCTGCCCAACGATAAAGGTCGGCAGGTAA‑GTGACCCCCATCCCGGCAATCGCAG‑CCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTGGGTTTTGCCACCTTTGTGGAATTGCCACTGCACCTGCCGCGAATGCCCATAAGGCAGGCAATCATGCGCGGCGAGCTCATC > NC_002947/4336385‑4336671 | gcCACCTGCCGGTGCTGCGGATACACCGCCGACAGCTGCAGCGCGGGCGGGCGCCACTCATCCAGAACCGTCACCAGTTGCCCCTCCGCCAACGCCTGCCCAACGATAAAGGTCGGCAGGTAA‑GTGACCCCCATCCCGGCAATCGCAG‑c < 3:197089/149‑1 (MQ=255) ctgcGGAAACACCGCCGCCAGCGGCAGCGCGGGCGGGCGCCACTCATCCAGAACCGTCACCAGTTGCCCCTCCGCCAACGCCTGCCCAACGATAAAGGTCGGCAGGTAA‑GTGACCCCCATCCCGGCAATCGCAGCCCCCCCGCAGCAAc < 4:224857/149‑1 (MQ=255) ccaccTGCCGCGCGGGCGGGCGCCACTCACCCACACCCGCCCCCAGTTCCCCCCCCCCCAACCCCTCCCCAACGAAAACGGTCGGCAGGTAA‑GTCACCCCCATCCCGGCAATCGCCGCCCCCCCGCCCCAACTCGCCATTGTTCCCCCg < 2:290085/145‑1 (MQ=255) cTGCAGCGCGGGCGGGCGCCACTCATCCAGAACCGTCACCAGTTGCCCATCCGCCAACGCCTGCCCAACGATAAAGGTCGGCAGGTAACTTGACCCCCATCCCGGCAATCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCAt < 1:95319/149‑1 (MQ=255) aTCCAGAACCGTCACCAGTTGCCCCTCCGCCAACGCCTGCCCAACGATAAAGGTCGGCAGGTAA‑GTGACCCCCATCCCGGCAATCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGc < 1:3437/145‑1 (MQ=255) aTCCAGAACCGTCACCAGTTGCCCCTCCGCCAACGCCTGCCCAACGATAAAGGTCGGCAGGTAA‑GTGACCCCCATCCCGGCAATCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTgg < 3:171391/149‑1 (MQ=255) ccAACGCCTGCCCAACGATAAAGGTCGGCAGGTAA‑GTGCGGCCCATCCCGGCAATCGCAGACCCCCCGCAGCCACTCGCCATTAGTAGCCCGCATGCGCCCGGTCACCAGAATAGCCATGGGTTTGAAAACATTGTGGAATTGcaaat > 6:100825/1‑144 (MQ=255) ggTCGGCAGGTAA‑GTGCCGCCCAGCCCGGCAATCGCAGCCCCCCCGCCGCGACGCGCCATTGTTCGCCCGCCTGCGCCCGGTCACCGGAATTGCCTGGGTGTTGGCGCCTTTGTGGAATTGCCACTGCACCTGCCGCGAATGCGCAt > 6:44380/1‑147 (MQ=255) gTGACCCCCATCCCGGCAATCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTGGGTTTTGCCACCTTTGTGGAATTGCCACTGCACCTGCCGCGAATGCCCATAAGGCAGGCAATCAt < 5:44380/149‑1 (MQ=255) tGACCCCCATCCCGGCAATCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTGGGTTTTGCCACCTTTGTGGAATTGCCACTGCACCTGCCGCGAATGCCCATAAGGCAGGCAATCATg < 7:81560/149‑1 (MQ=255) ccccATCCCGGCAATCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTGGGTTTTGCCACCTTTGTGGAATTGCCACTGCACCTGCcg > 8:305896/1‑118 (MQ=255) ccccATCCCGGCAATCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTGGGTTTTGCCACCTTTGTGGAATTGCCACTGCACCTGCcg < 7:305896/118‑1 (MQ=255) ccccATCCCGGCAATCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTGGGTTTTGCCACCTTTGTGGAATTGCCACTGCACCTGCCGCGAATGCCCATAAGGCAGGCAATCATGcgcg > 1:42733/1‑149 (MQ=255) cATCCCGGCAATCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTGGGTTTTGCCACCTTTGTGGAATTGCCACTGCACCTGCCGCGAATGCCCATAAGGCAGGCAATCATGCgcggc > 6:49855/1‑148 (MQ=255) aTCCCGGCAATCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTGGGTTTTGCCACCTTTGTGGAATTGCCACTGCACCTGCCGCGAATGCCCATAAGGCAGGCAATCATGCGCGGCGa < 5:49855/149‑1 (MQ=255) aTCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTGGGTTTTGCCACCTTTGTGGAATTGCCACTGCACCTGCCGCGAATGCCCATAAGGCAGGCAATCATGCGCGGCGAGCTCATc < 6:56570/147‑1 (MQ=255) aTCGCAGCCCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTGGGTTTTGCCACCTTTGTGGAATTGCCACTGCACCTGCCGCGAATGCCCATAAGGCAGGCAATCATGCGCGGCGAGCTCATc > 5:56570/1‑147 (MQ=255) | GCCACCTGCCGGTGCTGCGGATACACCGCCGACAGCTGCAGCGCGGGCGGGCGCCACTCATCCAGAACCGTCACCAGTTGCCCCTCCGCCAACGCCTGCCCAACGATAAAGGTCGGCAGGTAA‑GTGACCCCCATCCCGGCAATCGCAG‑CCCCCCGCAGCAACTCGCCATTGTTCGCCCGCATGCGCCCGGTCACCTGAATTGCCTGGGTTTTGCCACCTTTGTGGAATTGCCACTGCACCTGCCGCGAATGCCCATAAGGCAGGCAATCATGCGCGGCGAGCTCATC > NC_002947/4336385‑4336671 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |