Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,070,814 A→C 7.4% F25V (TTC→GTC)  PP_4466 ← TauD/TfdA family dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,070,8140AC 7.4% 157.7 / 4.9 54F25V (TTC→GTC) PP_4466TauD/TfdA family dioxygenase
Reads supporting (aligned to +/- strand):  ref base A (25/25);  new base C (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

TTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTCCAATAACA  >  NC_002947/5070668‑5070962
                                                                                                                                                  |                                                                                                                                                     
tttCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGaa                                                                                                                                                       <  7:275723/147‑1 (MQ=255)
tttCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGaa                                                                                                                                                       <  7:178759/147‑1 (MQ=255)
gttCGGGAGAATAACAGCTTCCCCGAAGTGATGACAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACCTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGaa                                                                                                                                                       <  6:175621/146‑1 (MQ=255)
      gTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTAGTTCAAGTCaaat                                                                                                                                               >  1:250643/1‑147 (MQ=255)
              cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATg                                                                                                                                      >  5:35875/1‑149 (MQ=255)
              cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATg                                                                                                                                      >  8:193888/1‑149 (MQ=255)
              cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATg                                                                                                                                      >  3:147261/1‑149 (MQ=255)
              cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGACTACGATg                                                                                                                                      >  3:142175/1‑149 (MQ=255)
              cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTAGTTGAAGTCAAAGCCTACGATg                                                                                                                                      >  4:14588/1‑149 (MQ=255)
              cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGAGACATCCTGAGCATCGTAGTCGTTGAAGTCAAATCCTACGATg                                                                                                                                      >  7:134119/1‑149 (MQ=255)
              cAGCTTCCCCGAAGTGATGAGACAACTTGATGTGCAGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACAGGAGCAAGATTTTTCGTACTTCGTAGACATCATGACCATCGAAGTCGTTTAAGACAAAGACTACGAta                                                                                                                                      >  4:242971/1‑148 (MQ=255)
               aGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGt                                                                                                                                     <  5:6027/149‑1 (MQ=255)
                gCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTc                                                                                                                                    >  2:43525/1‑149 (MQ=255)
                           gTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGacac                                                                                                                         <  5:219367/149‑1 (MQ=255)
                                 agagaACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGc                                                                                                                   >  4:206422/1‑149 (MQ=255)
                                 agagaACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGGCTTTGACTTCAACGACTACGATgctc                                                                                                                                   >  3:223165/1‑130 (MQ=38)
                                 agagaACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGGCTTTGACTTCAACGACTACGATgctc                                                                                                                                   <  4:223165/133‑4 (MQ=38)
                                        ttGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCga                                                                                                            >  7:245920/1‑149 (MQ=255)
                                          gATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGCGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCga                                                                                                            <  4:196668/147‑1 (MQ=255)
                                          gATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCgaga                                                                                                          >  6:327228/1‑149 (MQ=255)
                                                ctgctCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGcc                                                                                                     <  2:30617/148‑1 (MQ=255)
                                                ctgctCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGcc                                                                                                     <  8:245920/148‑1 (MQ=255)
                                                   ctCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGa                                                                                                 <  3:14588/149‑1 (MQ=255)
                                                     cATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATc                                                                                               <  8:5136/149‑1 (MQ=255)
                                                              tGTCGATATCGCGGAAACGTACAACACGGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTATTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTg                                                                                      <  3:242971/149‑1 (MQ=255)
                                                              tGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTg                                                                                      <  2:43204/149‑1 (MQ=255)
                                                               cgcGAAATCGCGCACACCAACAACCCCCCACTGGACCCACAGTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAACCCTACGATCTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTgg                                                                                     <  8:230003/147‑1 (MQ=255)
                                                                        gcgGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgt                                                                            <  5:309989/149‑1 (MQ=255)
                                                                        gcgGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgt                                                                            <  1:139218/149‑1 (MQ=255)
                                                                        gcgGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGAAACCACTGCCATTCGAGACTGCAGTGATCTTGTTTTTGGTCAGCAtgt                                                                            >  1:16837/1‑149 (MQ=255)
                                                                         cgGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgtt                                                                           >  3:58383/1‑149 (MQ=255)
                                                                           gAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgttcc                                                                         <  2:46079/149‑1 (MQ=255)
                                                                                gTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgttcctgttc                                                                    <  1:43525/149‑1 (MQ=255)
                                                                                gTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgttcctgttc                                                                    >  2:321943/1‑149 (MQ=255)
                                                                                 tACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCCTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgttcctgttcc                                                                   >  2:89518/1‑149 (MQ=255)
                                                                                    aacaCCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGAATGCCGTGATCTTGTTTTTGGTCAGCATgttcatgttcct                                                                  >  1:263735/1‑148 (MQ=255)
                                                                                                gAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAg                                                      <  3:206422/147‑1 (MQ=255)
                                                                                                           ttCGTACTTCGGCGACATCCTGAGCTTCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCtgt                                          <  6:35875/148‑1 (MQ=255)
                                                                                                           ttCGTACTTCGGCGACATCCTGAGCATCTTAGTCGTTGAAGTAAAAGCCTACGATGTCCTCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATTTTCCTGTTCCTCACCAGCTGAAGGTTACGATCtgt                                          <  8:134119/148‑1 (MQ=255)
                                                                                                               tACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCt                                      >  8:218157/1‑148 (MQ=255)
                                                                                                                 cTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCTTGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAAc                                   >  4:14538/1‑149 (MQ=255)
                                                                                                                 cTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTaa                                    <  2:250643/148‑1 (MQ=255)
                                                                                                                 cTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTaa                                    <  7:218157/148‑1 (MQ=255)
                                                                                                                   tCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAAcc                                  >  6:114649/1‑148 (MQ=255)
                                                                                                                        gACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGc                            >  4:71661/1‑149 (MQ=255)
                                                                                                                           aTCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGc                         <  5:114649/149‑1 (MQ=255)
                                                                                                                                 aGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTc                     <  2:263735/147‑1 (MQ=255)
                                                                                                                                  gCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAAc                  <  7:193888/149‑1 (MQ=255)
                                                                                                                                    aTCGTAGGCTTTGACTTCAACGACTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCg                           >  3:147033/1‑138 (MQ=255)
                                                                                                                                    aTCGTAGGCTTTGACTTCAACGACTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCg                           <  4:147033/138‑1 (MQ=255)
                                                                                                                                       gTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGa              <  4:58383/148‑1 (MQ=255)
                                                                                                                                       gTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGAc             >  8:151041/1‑149 (MQ=255)
                                                                                                                                          gTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTc          <  5:327228/149‑1 (MQ=255)
                                                                                                                                           tCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTc          >  5:80798/1‑148 (MQ=255)
                                                                                                                                              ttGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAAATCGACTTCCaa       >  5:25373/1‑148 (MQ=255)
                                                                                                                                               tGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCtg                                           <  6:191283/111‑1 (MQ=255)
                                                                                                                                               tGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCtg                                           >  5:191283/1‑111 (MQ=255)
                                                                                                                                                 aaGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTCCAATaa    <  1:321943/148‑1 (MQ=255)
                                                                                                                                                  aGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTCCAATAACa  <  6:25373/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTCCAATAACA  >  NC_002947/5070668‑5070962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: