Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,070,815 G→T 7.2% D24E (GAC→GAA)  PP_4466 ← TauD/TfdA family dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,070,8150GT 7.2% 167.8 / 4.2 56D24E (GAC→GAA) PP_4466TauD/TfdA family dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (25/27);  new base T (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01

GTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTCCAATAACAA  >  NC_002947/5070674‑5070963
                                                                                                                                              |                                                                                                                                                    
gTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTAGTTCAA‑GTCaaat                                                                                                                                                >  1:250643/1‑147 (MQ=255)
        cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATg                                                                                                                                       >  8:193888/1‑149 (MQ=255)
        cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATg                                                                                                                                       >  3:147261/1‑149 (MQ=255)
        cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATg                                                                                                                                       >  5:35875/1‑149 (MQ=255)
        cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGACTACGATg                                                                                                                                       >  3:142175/1‑149 (MQ=255)
        cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTAGTTGAA‑GTCAAAGCCTACGATg                                                                                                                                       >  4:14588/1‑149 (MQ=255)
        cAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGAGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAATCCTACGATg                                                                                                                                       >  7:134119/1‑149 (MQ=255)
        cAGCTTCCCCGAAGTGATGAGACAACTTGATGTGCAGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACAGGAGCAAGATTTTTCGTACTTCGTAGACATCATGACCATCGAAGTCGTTTAA‑GACAAAGACTACGAta                                                                                                                                       >  4:242971/1‑148 (MQ=255)
         aGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGt                                                                                                                                      <  5:6027/149‑1 (MQ=255)
          gCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTc                                                                                                                                     >  2:43525/1‑149 (MQ=255)
                     gTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGacac                                                                                                                          <  5:219367/149‑1 (MQ=255)
                           agagaACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGc                                                                                                                    >  4:206422/1‑149 (MQ=255)
                           agagaACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGGCTTTGAC‑TTCAACGACTACGATgctc                                                                                                                                    <  4:223165/133‑4 (MQ=38)
                           agagaACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGGCTTTGAC‑TTCAACGACTACGATgctc                                                                                                                                    >  3:223165/1‑130 (MQ=38)
                                  ttGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCga                                                                                                             >  7:245920/1‑149 (MQ=255)
                                    gATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGCGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCga                                                                                                             <  4:196668/147‑1 (MQ=255)
                                    gATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCgaga                                                                                                           >  6:327228/1‑149 (MQ=255)
                                          ctgctCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGcc                                                                                                      <  2:30617/148‑1 (MQ=255)
                                          ctgctCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGcc                                                                                                      <  8:245920/148‑1 (MQ=255)
                                             ctCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGa                                                                                                  <  3:14588/149‑1 (MQ=255)
                                               cATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATc                                                                                                <  8:5136/149‑1 (MQ=255)
                                                        tGTCGATATCGCGGAAACGTACAACACGGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTATTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTg                                                                                       <  3:242971/149‑1 (MQ=255)
                                                        tGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTg                                                                                       <  2:43204/149‑1 (MQ=255)
                                                         cgcGAAATCGCGCACACCAACAACCCCCCACTGGACCCACAGTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAACCCTACGATCTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTgg                                                                                      <  8:230003/147‑1 (MQ=255)
                                                                  gcgGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgt                                                                             <  5:309989/149‑1 (MQ=255)
                                                                  gcgGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgt                                                                             <  1:139218/149‑1 (MQ=255)
                                                                  gcgGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGAAACCACTGCCATTCGAGACTGCAGTGATCTTGTTTTTGGTCAGCAtgt                                                                             >  1:16837/1‑149 (MQ=255)
                                                                   cgGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgtt                                                                            >  3:58383/1‑149 (MQ=255)
                                                                     gAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgttcc                                                                          <  2:46079/149‑1 (MQ=255)
                                                                          gTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgttcctgttc                                                                     <  1:43525/149‑1 (MQ=255)
                                                                          gTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgttcctgttc                                                                     >  2:321943/1‑149 (MQ=255)
                                                                           tACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCCTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCAtgttcctgttcc                                                                    >  2:89518/1‑149 (MQ=255)
                                                                              aacaCCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAAGGTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGAATGCCGTGATCTTGTTTTTGGTCAGCATgttcatgttcct                                                                   >  1:263735/1‑148 (MQ=255)
                                                                                          gAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAg                                                       <  3:206422/147‑1 (MQ=255)
                                                                                                     ttCGTACTTCGGCGACATCCTGAGCTTCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCtgt                                           <  6:35875/148‑1 (MQ=255)
                                                                                                     ttCGTACTTCGGCGACATCCTGAGCATCTTAGTCGTTGAA‑GTAAAAGCCTACGATGTCCTCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATTTTCCTGTTCCTCACCAGCTGAAGGTTACGATCtgt                                           <  8:134119/148‑1 (MQ=255)
                                                                                                         tACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCt                                       >  8:218157/1‑148 (MQ=255)
                                                                                                           cTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCTTGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAAc                                    >  4:14538/1‑149 (MQ=255)
                                                                                                           cTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTaa                                     <  7:218157/148‑1 (MQ=255)
                                                                                                           cTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTaa                                     <  2:250643/148‑1 (MQ=255)
                                                                                                             tCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAAcc                                   >  6:114649/1‑148 (MQ=255)
                                                                                                                  gACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGc                             >  4:71661/1‑149 (MQ=255)
                                                                                                                     aTCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGc                          <  5:114649/149‑1 (MQ=255)
                                                                                                                           aGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTc                      <  2:263735/147‑1 (MQ=255)
                                                                                                                            gCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAAc                   <  7:193888/149‑1 (MQ=255)
                                                                                                                              aTCGTAGGCTTTGAC‑TTCAACGACTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCg                            >  3:147033/1‑138 (MQ=255)
                                                                                                                              aTCGTAGGCTTTGAC‑TTCAACGACTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCg                            <  4:147033/138‑1 (MQ=255)
                                                                                                                                 gTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGa               <  4:58383/148‑1 (MQ=255)
                                                                                                                                 gTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGAc              >  8:151041/1‑149 (MQ=255)
                                                                                                                                    gTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTc           <  5:327228/149‑1 (MQ=255)
                                                                                                                                     tCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTc           >  5:80798/1‑148 (MQ=255)
                                                                                                                                        ttGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAAATCGACTTCCaa        >  5:25373/1‑148 (MQ=255)
                                                                                                                                         tGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCtg                                            >  5:191283/1‑111 (MQ=255)
                                                                                                                                         tGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCtg                                            <  6:191283/111‑1 (MQ=255)
                                                                                                                                           aa‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTCCAATaa     <  1:321943/148‑1 (MQ=255)
                                                                                                                                            a‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTCCAATAACa   <  6:25373/149‑1 (MQ=255)
                                                                                                                                              gTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTCCAATAACaa  >  4:40576/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA‑GTCAAAGCCTACGATGTCCGCGCAGACACCACTGCCATTCGAGACTGCCGTGATCTTGTTTTTGGTCAGCATGTTCCTGTTCCTCACCAGCTGAAGGTTACGATCTGTGCCTAACCTACAGCGGCATTCAACTCGACTTCCAATAACAA  >  NC_002947/5070674‑5070963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑