Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,140,917 G→A 5.7% I215I (ATC→ATT)  PP_4525 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,140,9170GA 5.7% 213.7 / 2.4 73I215I (ATC→ATT) PP_4525LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (35/31);  new base A (2/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGAGTACCGCCGGGTGCTCGGGGCTGG  >  NC_002947/5140772‑5141062
                                                                                                                                                 |                                                                                                                                                 
gCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGAtc                                                                                                                                                >  4:84803/1‑149 (MQ=255)
gCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGAtc                                                                                                                                                <  5:65501/149‑1 (MQ=255)
    ggTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCa                                                                                                                                            >  8:149636/1‑149 (MQ=255)
      tGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATc                                                                                                                                          <  5:199467/149‑1 (MQ=255)
            ccTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGc                                                                                                                                    >  8:206201/1‑149 (MQ=255)
             cTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAAGCAGGCTGTCGAGCATCGACTGGGCCATCCTCGCCACACCCGCACCCGCGATCACACCGGCCCGCATGCTCTGGTACGACTCGATCTCCATCACCCGcc                                                                                                                                   >  1:243888/1‑149 (MQ=255)
              tGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGccc                                                                                                                                  >  5:113563/1‑149 (MQ=255)
                  cGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTcc                                                                                                                                             >  1:43550/1‑134 (MQ=255)
                  cGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTcc                                                                                                                                             <  2:43550/134‑1 (MQ=255)
                   gAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTg                                                                                                                             >  2:163477/1‑149 (MQ=255)
                    aaaGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCAt                                                                                                                               >  4:50318/1‑146 (MQ=255)
                    aaaGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCAt                                                                                                                               <  3:50318/146‑1 (MQ=255)
                     aaGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGc                                                                                                                           <  7:206201/149‑1 (MQ=255)
                         tGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCAGCGACTGGGCCATCATCG‑CACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTatg                                                                                                                      <  6:232714/149‑1 (MQ=255)
                           ccTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTatgg                                                                                                                     <  4:230525/149‑1 (MQ=255)
                           ccTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACCCGATCTCCATCACCCGCCCCATTGGCGTatgg                                                                                                                     <  8:153133/149‑1 (MQ=255)
                            cTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTatggg                                                                                                                    >  3:224890/1‑149 (MQ=255)
                                 gCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCCCACCCGCGATCACACAGGCAAGCATGCTCTGGTACGACTCGATGGCCATCCCCCGCCCCATGGGCGTatgggcaggg                                                                                                               >  8:204954/1‑149 (MQ=255)
                                        cACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTatgggcat                                                                                                                 <  7:165577/140‑1 (MQ=255)
                                        cACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTatgggcat                                                                                                                 >  8:165577/1‑140 (MQ=255)
                                           ccTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCTATCTCCATCACCCGCCCCATTGCCGTATGGGCATGGGCATACCAgg                                                                                                     <  7:204954/149‑1 (MQ=255)
                                           ccTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAgg                                                                                                     >  5:24392/1‑149 (MQ=255)
                                            cTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGc                                                                                                    >  5:213003/1‑149 (MQ=255)
                                                cccGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTc                                                                                                 >  7:157842/1‑148 (MQ=255)
                                                 ccGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCa                                                                                               >  3:260864/1‑149 (MQ=255)
                                                 ccGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCa                                                                                               <  7:149636/149‑1 (MQ=255)
                                                  cGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCCGCATGCTCTGGTACGCGTCGAGCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGcagc                                                                                                 >  4:127262/1‑143 (MQ=255)
                                                   gATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCAATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGa                                                                                             >  3:183008/1‑149 (MQ=255)
                                                   gATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCAATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGa                                                                                             >  3:178751/1‑149 (MQ=255)
                                                    ctCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGAc                                                                                            <  8:138540/148‑1 (MQ=255)
                                                     tCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACg                                                                                           >  1:124623/1‑149 (MQ=255)
                                                       aCGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCa                                                                                         >  3:126053/1‑149 (MQ=255)
                                                         gCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATg                                                                                       >  3:143531/1‑149 (MQ=255)
                                                         gCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATg                                                                                       >  5:273377/1‑149 (MQ=255)
                                                               gcaggcTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTAc                                                                                 <  3:127262/149‑1 (MQ=255)
                                                                 aggcTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGa                                                                               <  3:84803/149‑1 (MQ=255)
                                                                       tCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGagcagc                                                                          <  6:24392/148‑1 (MQ=255)
                                                                         gAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGagca                                                                            <  1:161485/144‑1 (MQ=255)
                                                                         gAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGagca                                                                            >  2:161485/1‑144 (MQ=255)
                                                                         gAGCATCGACTGGGCCATCATCGCCACACCCACACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCt                                                                       >  4:117318/1‑149 (MQ=255)
                                                                           gCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTgg                                                                     >  6:126348/1‑149 (MQ=255)
                                                                            cATGGAGTGGGCCATCATCGAGACACCCGCACCCGGGTTCAGACAGGCCAGGATGGTCTGGTAGGACTGTATATCCATCTCCCGCGCCTTTGGCGTATGGGCATGGGCATAGCTGGCCTCCAGACGCATGGGGTACTAGCAGCCCttt                                                                     <  4:260864/148‑3 (MQ=255)
                                                                            cATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGc                                                                    <  7:51488/149‑1 (MQ=255)
                                                                                gACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGa                                                                 <  1:163477/148‑1 (MQ=255)
                                                                                       ccATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAac                                                         <  6:273377/149‑1 (MQ=255)
                                                                                        catcatCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAaca                                                        >  3:30797/1‑149 (MQ=255)
                                                                                                ccACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTat                                                                                                                       >  7:218515/1‑78 (MQ=255)
                                                                                                ccACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTg                                                >  6:136848/1‑149 (MQ=255)
                                                                                                acacaCCCTCACCCGAGATCACACAGGCAATCATGCTCTGGTACGACTCGATCTCCATCACCCGCACCATTGGCGTat                                                                                                                       <  8:218515/77‑1 (MQ=255)
                                                                                                    acccgcacccgcGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCg                                            >  3:139684/1‑149 (MQ=255)
                                                                                                     cccgcacccgcGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGc                                           <  5:126348/149‑1 (MQ=255)
                                                                                                     cccgcacccgcGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGc                                           >  1:252762/1‑149 (MQ=255)
                                                                                                      ccgcacccgcGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGcc                                          <  2:124623/149‑1 (MQ=255)
                                                                                                       cgcacccgcGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCa                                         <  4:143531/149‑1 (MQ=255)
                                                                                                            ccgcGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGc                                               >  2:51942/1‑138 (MQ=255)
                                                                                                            ccgcGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGc                                               <  1:51942/138‑1 (MQ=255)
                                                                                                                  tCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCg                              <  3:117318/149‑1 (MQ=255)
                                                                                                                    acacaGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGt                            <  4:139684/149‑1 (MQ=255)
                                                                                                                     cacaGGCCACCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTg                           <  2:243888/149‑1 (MQ=255)
                                                                                                                      acaGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCAGGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGa                          <  8:7010/149‑1 (MQ=255)
                                                                                                                       caGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGAg                         >  3:45060/1‑149 (MQ=255)
                                                                                                                             aGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGAGTAccgc                   >  8:255559/1‑149 (MQ=255)
                                                                                                                                aTGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGAGTACCGCCgg                >  5:49371/1‑149 (MQ=255)
                                                                                                                                aTGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGAGTACCGCCgg                <  4:126053/149‑1 (MQ=255)
                                                                                                                                       ggTACGACTCCATCTCCATCACCCGCCCCATTGGCGTATGGCCATGGGCATACCAGGCCTCCAGACGCATCCGGTACGAGCAGCCCTGGCGGAAGGTCACCACCGCCTTGCCCGCCACGTCCTTGTCGGTGACTACCGCCGGGTGCTCg         >  8:14024/1‑149 (MQ=255)
                                                                                                                                        gTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGAGTACCGCCGGGTGCTc          <  6:213003/147‑1 (MQ=255)
                                                                                                                                        gTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACAACGCCTTGCCCGCCACGTCCTTGGCGGTGAGTACCGCCGGGTGCTCgg        >  8:179877/1‑149 (MQ=255)
                                                                                                                                            gACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCt                                   >  4:314078/1‑118 (MQ=255)
                                                                                                                                            gACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCt                                   <  3:314078/118‑1 (MQ=255)
                                                                                                                                             aCTCAATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGAGTACCGCCGGGTGCTCGGGGCTg   <  4:183008/149‑1 (MQ=255)
                                                                                                                                             aCTCAATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGAGTACCGCCGGGTGCTCGGGGCTg   <  4:178751/149‑1 (MQ=255)
                                                                                                                                              cTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGAGTACCGCCGGGTGCTCGGGGCTgg  >  7:101600/1‑149 (MQ=255)
                                                                                                                                              cTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGAGTACCGCCGGGTGCTCGGGGCTgg  <  4:45060/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGTGAACACCGCCTTGCCCGCCACGTCCTTGGCGGTGAGTACCGCCGGGTGCTCGGGGCTGG  >  NC_002947/5140772‑5141062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑