Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,166,530 C→T 7.8% L450L (CTG→CTA) ‡ hrpA ← ATP‑dependent helicase HrpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,166,5300CT 7.8% 144.3 / 2.7 51L450L (CTG→CTA) ‡hrpAATP‑dependent helicase HrpA
Reads supporting (aligned to +/- strand):  ref base C (22/25);  new base T (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

GGTCGATCGGCAAGCGCGCCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGACGCACGCAAATGCCCGGCTCGACCCGGCCGCAACGGCCTTTAC  >  NC_002947/5166382‑5166678
                                                                                                                                                    |                                                                                                                                                    
ggTCGATCGGCAAGCGCGCCAACTGGCGGCCAATTGGCGGCAGGTGGTTCTCGCGGTTAACCGCCTAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCGCGATGAACGGGAAGGCTTCGATTGCGccc                                                                                                                                                      <  6:279505/149‑1 (MQ=255)
    gATCGGCAAGCGCGCCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGgc                                                                                                                                                  <  3:142761/149‑1 (MQ=255)
     aTCGGCAAGCGCGCCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGgcg                                                                                                                                                 <  4:127566/149‑1 (MQ=255)
     aTCGGCAAGCGCGCCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGgcg                                                                                                                                                 >  8:220998/1‑149 (MQ=255)
        ggCAAGCGCGCCAACTGGCGGCCAATCGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAg                                                                                                                                              <  1:55625/149‑1 (MQ=255)
         gCAAGCGCGCCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCTGGGCGCaat                                                                                                                                             >  5:210458/1‑147 (MQ=255)
         gCAAGCGCGCCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAgg                                                                                                                                             >  7:172579/1‑149 (MQ=255)
          cAAGCGCGCCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGt                                                                                                                                            <  6:130366/149‑1 (MQ=255)
           aaGCGCGCCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCTGGGCGCaatcg                                                                                                                                           <  6:210458/149‑5 (MQ=255)
               gcgcCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCaa                                                                                                                                        >  3:113827/1‑148 (MQ=255)
                 gcCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGc                                                                                                                                          <  4:234740/144‑1 (MQ=255)
                 gcCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGc                                                                                                                                          >  3:234740/1‑144 (MQ=255)
                 gcCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACAt                                                                                                                                     <  7:186228/149‑1 (MQ=255)
                   cAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCt                                                                                                                                   <  7:174020/149‑1 (MQ=255)
                     aCTGGCTGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTTATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGc                                                                                                                                 <  5:15426/149‑1 (MQ=255)
                     aCTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGc                                                                                                                                 <  2:321878/149‑1 (MQ=255)
                               aaTTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCAccg                                                                                                                       >  7:869/1‑149 (MQ=255)
                                    gCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCgccgcc                                                                                                                  <  8:172579/149‑1 (MQ=255)
                                              gTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTgc                                                                                                        <  7:220998/149‑1 (MQ=255)
                                                 ctcGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGt                                                                                                              <  3:272705/140‑1 (MQ=255)
                                                 ctcGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGt                                                                                                              >  4:272705/1‑140 (MQ=255)
                                                  tcGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTgcg                                                                                                       >  8:4230/1‑146 (MQ=255)
                                                   cgcgGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAg                                                                                                    >  7:223955/1‑148 (MQ=255)
                                                    gcgGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCTGCCAg                                                                                                                <  1:47235/135‑1 (MQ=255)
                                                    gcgGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCTGCCAg                                                                                                                >  2:47235/1‑135 (MQ=255)
                                                           aCCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCgg                                                                                           >  3:261629/1‑149 (MQ=255)
                                                             cgccgAAAGCTCCTGCAACAGCGTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGt                                                                                         <  8:278360/149‑1 (MQ=255)
                                                               ccgAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCg                                                                                       >  6:313226/1‑149 (MQ=255)
                                                                            cAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGcc                                                                          >  1:23718/1‑149 (MQ=255)
                                                                                  gTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGt                                                                    >  1:115860/1‑149 (MQ=255)
                                                                                   ttGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGtt                                                                   >  4:96047/1‑149 (MQ=255)
                                                                                     gAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTg                                                                  >  2:303156/1‑148 (MQ=255)
                                                                                      aaGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGaa                                                                <  7:4230/149‑1 (MQ=255)
                                                                                         ccGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTc                                                             >  3:9051/1‑149 (MQ=255)
                                                                                         ccGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTc                                                             <  2:115860/149‑1 (MQ=255)
                                                                                                tGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCAc                                                                                                                         <  8:25633/82‑1 (MQ=255)
                                                                                                tGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCAc                                                                                                                         >  7:25633/1‑82 (MQ=255)
                                                                                                tGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCctct                                                         <  4:113827/146‑1 (MQ=255)
                                                                                                     gCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTa                                                 <  8:869/149‑1 (MQ=255)
                                                                                                     gCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTa                                                 <  3:96047/149‑1 (MQ=255)
                                                                                                     gCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTa                                                 <  1:303156/149‑1 (MQ=255)
                                                                                                             aTCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTg                                                                  <  1:286605/124‑1 (MQ=255)
                                                                                                             aTCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTg                                                                  >  2:286605/1‑124 (MQ=255)
                                                                                                                           tGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGACGCACGCAAATGCCCGGc                           >  7:98193/1‑149 (MQ=255)
                                                                                                                                 ggAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGACGCACGCAAATGCCCGGCTCGAcc                     <  8:98193/149‑1 (MQ=255)
                                                                                                                                      gCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGACGCACGCAAATGCCCGGCTCGACCCGGcc                <  2:23718/149‑1 (MQ=255)
                                                                                                                                       cATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGT‑AACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGACGCACGCAAATGCCCGGCTCGACCCGGCCg               >  8:265926/1‑148 (MQ=255)
                                                                                                                                        aTCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGACGCACGCAAATGCCCGGCTCGACCCGGCCg               >  3:55554/1‑148 (MQ=255)
                                                                                                                                         tCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGACGCACGCAAATGCCCGGCTCGACCCGGCCGc              >  7:196493/1‑148 (MQ=255)
                                                                                                                                           acctGCGCCTGGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGac                                            <  8:16422/113‑1 (MQ=255)
                                                                                                                                           acctGCGCCTGGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGac                                            >  7:16422/4‑116 (MQ=255)
                                                                                                                                               gcgcCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGACGCACGCAAATGCCCGGCTCGACCCGGCCGCAACGGc        <  4:9051/148‑1 (MQ=255)
                                                                                                                                                    cAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGACGCACGCAAATGCCCGGCTCGACCCGGCCGCAACGGCCTTTAc  <  4:261629/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGTCGATCGGCAAGCGCGCCAACTGGCGGCCAATTGGCGTCAGCTGGTTCTCGCGGTTAACCGCCGAAAGCTCCTGCAACAGGTTGAAGCCGTCGCTGATGGCTTTGCCATCCGGCGGCTCGATGAACGGGAAGGCATCGATTGCGCCCAGGCGCAGGTGCAACATCTGCAGGATCACCGCCGCCAGGTTGGTGCGCAGAATCTCCGGGTCGGTAAACGCTGGCCGGCTGTTGAAGTCCTCTTCGCTGTAGAGACGCACGCAAATGCCCGGCTCGACCCGGCCGCAACGGCCTTTAC  >  NC_002947/5166382‑5166678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑