Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,298,506	T→C	11.3%	E532E (GAA→GAG)	recD ←	multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,298,506	0	T	C	11.3%	131.9 / 8.2	53	E532E (GAA→GAG)	recD	multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base C (3/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

GCAGGGCAATACCGATGTCACCGTTCATCAGGCCAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCAAGGGTGCGCAGGTAGC  >  NC_002947/5298361‑5298654                                                                                                                                                  |                                                                                                                                                      gcAGGGCAATACCGATGTCACCGTTCATCAGGCCAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCCTc                                                                                                                                                     >  6:41673/1‑148 (MQ=255) gcAGGGCAATACCGATGTCACCGTTCATCAGGCCAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGATGCTGGCTGTCGATAAGACCGGCATTGTGCACCACTCGCGCCACCCGCTCGTTGAGGCCTTCg                                                                                                                                                    >  8:261960/1‑149 (MQ=255)    gggCAATACCGATGTCACCGTTCATCAGGCCAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGaca                                                                                                                                                 >  4:183753/1‑149 (MQ=255)             cGATGTCACCGTTCATCAGGCCAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGac                                                                                                                                                  >  8:137635/1‑139 (MQ=255)             cGATGTCACCGTTCATCAGGCCAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGac                                                                                                                                                  <  7:137635/139‑1 (MQ=255)             cGATGTCACCGTTCATCAGGCCAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGgcg                                                                                                                                       >  6:39452/1‑149 (MQ=255)              gATGTCACCGTTCATCAGGCCAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGgcgc                                                                                                                                      >  8:87933/1‑149 (MQ=255)                tGTCACCGTTCATCAGGCCAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGccc                                                                                                                                    <  8:71860/149‑1 (MQ=255)                  tCACCGTTCATCAGGCCAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCCTCGACAC‑CCCAGGCGcccc                                                                                                                                   <  5:41673/148‑1 (MQ=255)                                  cAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGc                                                                                                                  <  3:223784/149‑1 (MQ=255)                                   aaGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGc                                                                                                                  >  4:51694/1‑148 (MQ=255)                                    aGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCCTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCaa                                                                                                                >  5:272884/1‑149 (MQ=255)                                             tCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATc                                                                                                       <  3:218824/149‑1 (MQ=255)                                                    gggTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCaaa                                                                                                 >  2:303218/1‑148 (MQ=255)                                                      gTCACCAGCACCGGGCGCCATTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACt                                                                                              <  1:303218/149‑1 (MQ=255)                                                       tCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTATCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAg                                                                                                                   <  3:965/127‑1 (MQ=255)                                                       tCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTATCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAg                                                                                                                   >  4:965/1‑127 (MQ=255)                                                                 cGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCCTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCt                                                                                   <  6:272884/149‑1 (MQ=255)                                                                  gggCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCATCTGGAAATCCTCAAAACTGCGCAACACCtt                                                                                  <  1:27421/149‑1 (MQ=255)                                                                  gggCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAac                                                                                       >  8:169708/1‑144 (MQ=255)                                                                  gggCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAac                                                                                       <  7:169708/144‑1 (MQ=255)                                                                             gTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCAtt                                                                       <  6:203870/149‑1 (MQ=255)                                                                                ccAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCt                                                                    >  2:124857/1‑149 (MQ=255)                                                                                           ggctgTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCgggg                                                         >  8:141940/1‑149 (MQ=255)                                                                                            gctgTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCggg                                                          <  5:39452/147‑1 (MQ=255)                                                                                              tgTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTgcgc                                                                                          <  6:86265/113‑1 (MQ=255)                                                                                              tgTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTgcgc                                                                                          >  5:86265/1‑113 (MQ=255)                                                                                                   aTCAGACCGGCATTGTGCAGCACTCGCGCCCCCCGCTCGTTGAGGCCTTCGACCC‑CCCAGGCGCCCCGGCGCACCTCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCATCCCCGCCCATAGCTCCCAGGCGCGGTCGACa                                                   >  7:130508/1‑147 (MQ=255)                                                                                                   aTCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACACAGCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAAATGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCa                                                   >  1:255795/1‑148 (MQ=255)                                                                                                   aTCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCaaa                                                 <  4:71473/149‑1 (MQ=255)                                                                                                       gACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCg                                             >  4:42313/1‑149 (MQ=255)                                                                                                       gACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCg                                             <  7:87933/149‑1 (MQ=255)                                                                                                          cGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTa                                          >  4:103733/1‑149 (MQ=255)                                                                                                           ggCATTGTGTTATACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTAt                                         <  1:277678/149‑1 (MQ=255)                                                                                                           ggCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTAt                                         <  1:124857/149‑1 (MQ=255)                                                                                                            gCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATc                                        <  3:183753/149‑1 (MQ=255)                                                                                                                        aCTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTgccggccg                            <  8:264343/149‑1 (MQ=255)                                                                                                                         cTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGccggccgg                           <  3:51694/149‑1 (MQ=255)                                                                                                                            gcgcCACCCGCTCGTTGAGGCCCCCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTaa                        >  7:301891/1‑149 (MQ=255)                                                                                                                                   ccGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTgcc                                 <  6:315079/133‑1 (MQ=255)                                                                                                                                   ccGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTgcc                                 >  5:315079/1‑133 (MQ=255)                                                                                                                                   ccGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGcc                   >  2:60409/1‑147 (MQ=255)                                                                                                                                   ccGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCaa                 <  7:141940/149‑1 (MQ=255)                                                                                                                                     gCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCAAgg               <  3:80791/149‑1 (MQ=255)                                                                                                                                       tCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCTGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCAAGGGt             <  3:103733/149‑1 (MQ=255)                                                                                                                                       tCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCAAGGGt             >  4:181472/1‑149 (MQ=255)                                                                                                                                             aGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCAAGGGTGCGCAg       <  1:60409/149‑1 (MQ=255)                                                                                                                                             aGGCCCCCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCAAGGGTGCGCa        <  8:301891/148‑1 (MQ=255)                                                                                                                                               gCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTGGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCAAGGGTGCGCTGGt     >  4:197762/1‑149 (MQ=255)                                                                                                                                                 cTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATc                                        >  5:75538/1‑112 (MQ=255)                                                                                                                                                 cTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATc                                        <  6:75538/112‑1 (MQ=255)                                                                                                                                                 cTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCAAGGGTGCGCAGGTAg   >  4:137317/1‑149 (MQ=255)                                                                                                                                                 cTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCAAGGGTGCGCAGGTAg   >  1:231962/1‑149 (MQ=255)                                                                                                                                                  ttCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCAAGGGTGCGCAGGTAGc  >  4:124452/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                      GCAGGGCAATACCGATGTCACCGTTCATCAGGCCAAGGCCGTAGTCGTTGCGGGTCACCAGCACCGGGCGCCCTTCGTACCAGGGCTGCTGGCTGTCGATCAGACCGGCATTGTGCAGCACTCGCGCCACCCGCTCGTTGAGGCCTTCGACAC‑CCCAGGCGCCCCGGCGCACCGCGCACAGCAACTGGAAATCCTCAAAACTGCGCAACACCTTGCCCGCCCATTGCTCCCAGGCGGGGTCGTCAAAGGCGGTATCAAGTGCCGGCCGGTAACGGCCAAGGGTGCGCAGGTAGC  >  NC_002947/5298361‑5298654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑