Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,361,071 A→T 13.8% intergenic (‑78/+23) PP_t49 ← / ← PP_t48 tRNA‑Met/tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,361,0710AT13.8% 142.7 / 13.1 58intergenic (‑78/+23)PP_t49/PP_t48tRNA‑Met/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (26/24);  new base T (2/6);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

GGGGGCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTGA  >  NC_002947/5360924‑5361216
                                                                                                                                                   |                                                                                                                                                 
gggggCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTa                                                                                                                                                   >  6:84455/1‑148 (MQ=255)
      ttgtagtGAACCAACGACCTTCGGGATATGAGCCAGACGATCTACGAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACGCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCggg                                                                                                                                            <  7:261134/143‑1 (MQ=255)
      tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCggg                                                                                                                                            <  7:107376/149‑1 (MQ=255)
        gATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGc                                                                                                                                           <  4:108958/148‑1 (MQ=255)
           ttGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCgg                                                                                                                                             <  8:246030/143‑1 (MQ=255)
           ttGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCgg                                                                                                                                             >  7:246030/1‑143 (MQ=255)
            tGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTAGAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGCtttc                                                                                                                                       <  5:327111/148‑1 (MQ=255)
             gacccAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAATCTGGGGCAAAAGTATAAGACCTAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGCtttct                                                                                                                                      <  1:36933/145‑2 (MQ=38)
              aaCCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGCtttcttg                                                                                                                                    <  5:194495/149‑4 (MQ=255)
              aaCCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGCtttctt                                                                                                                                     <  6:301475/148‑3 (MQ=255)
               aCCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGc                                                                                                                                   <  2:322238/149‑1 (MQ=255)
                ccAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGCtttcttgta                                                                                                                                  <  1:132694/149‑6 (MQ=255)
                  aaCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCtt                                                                                                                                 >  7:198370/1‑148 (MQ=255)
                   aCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTAACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCttt                                                                                                                                >  6:271754/1‑148 (MQ=255)
                     gACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAAt                                                                                                                             <  5:84455/149‑1 (MQ=255)
                      aCCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACTAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAAtt                                                                                                                            <  2:259689/149‑1 (MQ=255)
                        cTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTgg                                                                                                                          >  3:41699/1‑149 (MQ=255)
                        cTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTgg                                                                                                                          <  2:251377/149‑1 (MQ=255)
                                    aGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAggg                                                                                                              >  3:293722/1‑149 (MQ=255)
                                      cccGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGa                                                                                                            <  4:293722/149‑1 (MQ=255)
                                       ccGACGAGCTACCAAGCTGCTACACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGTGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGAc                                                                                                           <  8:198370/149‑1 (MQ=255)
                                            gaGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGaa                                                                                                      >  1:93122/1‑149 (MQ=255)
                                                   cAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTac                                                                                               <  4:41699/149‑1 (MQ=255)
                                                    aaGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCttt                                                                                                                                >  6:323557/1‑115 (MQ=255)
                                                    aaGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCttt                                                                                                                                <  5:323557/115‑1 (MQ=255)
                                                     aGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTacac                                                                                             >  5:279568/1‑149 (MQ=255)
                                                           tCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATg                                                                                       >  5:143736/1‑149 (MQ=255)
                                                            ccACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCCAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATgg                                                                                      <  4:321771/149‑1 (MQ=255)
                                                               ccccGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGTGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGca                                                                                   <  6:86195/149‑1 (MQ=255)
                                                                 ccGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAGGGGGACTCGACCCCCTCCACCCTATGGGcac                                                                                  >  6:229440/1‑148 (MQ=255)
                                                                   gcgACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAac                                                                               >  3:137202/1‑149 (MQ=255)
                                                                              ggggCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCg                                                                                                                                              <  5:209297/75‑1 (MQ=255)
                                                                              ggggCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCg                                                                                                                                              >  6:209297/1‑75 (MQ=255)
                                                                                       gTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCt                                                           <  5:271754/149‑1 (MQ=255)
                                                                                             gACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCaa                                                      >  3:242690/1‑148 (MQ=255)
                                                                                              aCCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAccc                                                                                                   >  7:281540/1‑102 (MQ=255)
                                                                                              aCCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAccc                                                                                                   <  8:281540/102‑1 (MQ=255)
                                                                                                     ccTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCcaccac                                             >  5:169464/1‑149 (MQ=255)
                                                                                                                tCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACACCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtac                                  >  5:333316/1‑149 (MQ=255)
                                                                                                                   aaTCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtacta                                >  2:173344/1‑148 (MQ=255)
                                                                                                                       cAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTAAACCCTATGGGCACAACCACCTCAAGGTTGCGAGTCTACCAATTCCACAACCTAAGAAATactactact                           >  8:275491/1‑149 (MQ=255)
                                                                                                                        aaaCATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtactactac                            <  1:238559/147‑1 (MQ=255)
                                                                                                                        aaaCATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtactactac                            >  2:238559/1‑147 (MQ=255)
                                                                                                                                aCCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAAccc                   <  4:242690/148‑1 (MQ=255)
                                                                                                                                  cTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTg                                                                  >  5:61942/1‑99 (MQ=255)
                                                                                                                                  cTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTg                                                                  <  6:61942/99‑1 (MQ=255)
                                                                                                                                      aaGAAAGCCCGCTAAAGCGGTCTCTCAAGCTTTAATTGGTACCGACAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACtt            <  7:275491/149‑1 (MQ=255)
                                                                                                                                        gAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCACTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCt          >  2:100582/1‑149 (MQ=255)
                                                                                                                                         aaaGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTg         >  6:158928/1‑149 (MQ=255)
                                                                                                                                          gaGCCCGCTTTAGCGGGCTTTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACACTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCACGGCAATACTACTACTTGAAACCCCTTACttat          >  4:278363/2‑145 (MQ=255)
                                                                                                                                          aaGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCCCCACCTCGGCAATACTACTACTTGAAACCCGGTACTTCTGc        >  3:239822/1‑149 (MQ=255)
                                                                                                                                             cccGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGc                                       <  2:29648/115‑1 (MQ=255)
                                                                                                                                             cccGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGc                                       >  1:29648/1‑115 (MQ=255)
                                                                                                                                              ccGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTa                                                          <  5:107917/95‑1 (MQ=255)
                                                                                                                                              ccGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTa                                                          >  6:107917/1‑95 (MQ=255)
                                                                                                                                               cGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCtt    >  5:194175/1‑148 (MQ=255)
                                                                                                                                                gCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACtt            >  6:321173/1‑139 (MQ=255)
                                                                                                                                                gCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACtt            <  5:321173/139‑1 (MQ=255)
                                                                                                                                                gCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTGa  <  6:169464/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGGGGCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTGA  >  NC_002947/5360924‑5361216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑