Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,376,873 T→C 100% D231G (GAC→GGC)  dnaK ← chaperone protein DnaK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,376,8730TC95.3% 125.6 / ‑2.1 42D231G (GAC→GGC) dnaKchaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (21/19);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGGTGTGGTCGCCCTTGGCCTT  >  NC_002947/5376737‑5377018
                                                                                                                                        |                                                                                                                                                 
aGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCAcc                                                                                                                                         >  5:57828/1‑147 (MQ=255)
  ttaccgtcAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAg                                                                                                                                      <  2:199113/141‑1 (MQ=255)
   ggACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTAGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCacg                                                                                                                                     >  8:300545/1‑146 (MQ=255)
    gacgacAGCTCTCTCGTGGCATTTTCTGCGGCTTCTTTCAGACGCGGCAGGGCCCGAGGATCGTTCTTCAGGTCCTTGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGGCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGaa                                                                                                                                   <  4:220836/149‑1 (MQ=255)
            cTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGc                                                                                                                           >  4:283115/1‑149 (MQ=255)
               gATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGt                                                                                                                        <  5:287200/149‑1 (MQ=255)
                      gCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGc                                                                                                                 <  6:57828/149‑1 (MQ=255)
                       cTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGcc                                                                                                                >  6:180400/1‑149 (MQ=255)
                                cGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCg                                                                                                       <  3:283115/149‑1 (MQ=255)
                                      cttTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTg                                                                                                  <  1:135128/148‑1 (MQ=255)
                                         aaaGAAGCCGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGt                                                                                                <  5:161368/145‑1 (MQ=255)
                                                        ccAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCg                                                                               <  8:290405/149‑1 (MQ=255)
                                                         cAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCgg                                                                              >  1:33618/1‑149 (MQ=255)
                                                              gATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGAtt                                                                         >  1:227659/1‑149 (MQ=255)
                                                                 cGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGAttt                                                                        <  2:161992/147‑1 (MQ=255)
                                                                 cGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGAttt                                                                        >  1:161992/1‑147 (MQ=255)
                                                                   ttcttcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCAccc                                                                                                                                        <  3:200056/81‑1 (MQ=255)
                                                                   ttcttcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCAccc                                                                                                                                        >  4:200056/1‑81 (MQ=255)
                                                                      ttcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGAc                                                                 >  1:109033/1‑149 (MQ=255)
                                                                      ttcAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGAc                                                                 >  4:45476/1‑149 (MQ=255)
                                                                        cAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGAcc                                                                <  3:45476/148‑1 (MQ=255)
                                                                                tGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTc                                                        >  4:196966/1‑148 (MQ=255)
                                                                                   ccGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAg                                                    >  5:257347/1‑149 (MQ=255)
                                                                                    cGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAgg                                                   <  2:227659/149‑1 (MQ=255)
                                                                                    cGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACAGTAGACTTCGGCCATTTCGATGACCGAAACGTCGAAgg                                                   >  5:44785/1‑149 (MQ=255)
                                                                                     gACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGt                                                  <  5:180400/149‑1 (MQ=255)
                                                                                         ctttcttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGGAGGTac                                                <  3:175570/147‑1 (MQ=255)
                                                                                         ctttcttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGGAGGTac                                                >  4:175570/1‑147 (MQ=255)
                                                                                          tttcttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTaccac                                             >  3:239884/1‑149 (MQ=255)
                                                                                          tttcttGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTAACCGTCGAATTCGGCGATTTAGATGACCGAAACGTCGAAGGTaccac                                             >  8:205065/1‑149 (MQ=255)
                                                                                          tttcttGAAATCGTCGACAACGTAGTCTATCATGCGAATGTCGAAGCCATCACCACCAAGGAAGGTCTCGCGGAAGGTAGCCAGTAATTCCAACTGGTGCTCACCGTCGACTTCAGCGATTTAGATGAAAGAAAAGTCGAAATTacca                                              >  8:228504/1‑148 (MQ=255)
                                                                                                 aaCTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAg                                      >  3:292804/1‑149 (MQ=255)
                                                                                                  aCTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAgg                                     <  8:192026/149‑1 (MQ=255)
                                                                                                    tcgtcgACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGt                                    >  5:305062/1‑148 (MQ=255)
                                                                                                         gACATGGTAGTCGATCATGCGCAGTTCGAAGCCTTCTCCACCCAGGAAGGTGTCTCCGTTGGTAGCCAGTACTTCGAACTTGTGCTCACAGTCGACTTCTGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATaaa                              <  7:228504/149‑1 (MQ=255)
                                                                                                         gACAAGGTAGTCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATaa                               <  7:300545/148‑1 (MQ=255)
                                                                                                                   tCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGgt                    <  2:33618/149‑1 (MQ=255)
                                                                                                                   tCGATCAGGCGCATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGgt                    >  3:11314/1‑149 (MQ=255)
                                                                                                                      aTCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGGtgtg                 >  2:310065/1‑149 (MQ=255)
                                                                                                                      aTCAGGCGAATGTCGAATCCTTCGCCACCCAGGAAGGTGTCTCCTTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGGtgtg                 <  6:44785/149‑1 (MQ=255)
                                                                                                                             gcATGTCGAAGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGGTGTGGTCGccc          >  1:146308/1‑149 (MQ=255)
                                                                                                                                     aaGCCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATTACGGTGTGGTCGCCCTTGGCCtt  <  7:205065/149‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                 
AGCGGACGACAGCTCGATCTTGGCTTTTTCTGCGGCTTCTTTCAGACGCTGCAGGGCCAGAGGATCGTTCTTCAGGTCCATGCCCGACTCTTTCTTGAACTCGTCGACAAGGTAGTCGATCAGGCGCATGTCGAAGTCTTCGCCACCCAGGAAGGTGTCGCCGTTGGTAGCCAGTACTTCGAACTGGTGCTCACCGTCGACTTCGGCGATTTCGATGACCGAAACGTCGAAGGTACCACCACCCAGGTCATAAACGATGACGGTGTGGTCGCCCTTGGCCTT  >  NC_002947/5376737‑5377018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑