Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,388,019	T→C	7.4%	L510P (CTC→CCC)	dld2 →	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,388,019	0	T	C	7.4%	153.0 / 2.9	54	L510P (CTC→CCC)	dld2	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (26/24);  new base C (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TGGAGTTTGGCGGCTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCC  >  NC_002947/5387884‑5388166                                                                                                                                        |                                                                                                                                                     ttgAGTTTGGCGGCTAGCTCAAGGCCGAGCACGGCACCGGGAGCAACATTGCGCGCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGTAAGCTCAAGCGCCTGCTATACCCCAACGTCATACTCATCCCCGACgt‑g                                                                                                                                        <  8:153734/147‑1 (MQ=255) tGGAGTTTGGCGGCTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACgt‑g                                                                                                                                        <  5:290123/149‑1 (MQ=255)      tttGGCGGCTGGCTGAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGtt                                                                                                                                   <  3:208582/149‑1 (MQ=255)       ttGGCGGCTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGGCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGtt                                                                                                                                   <  2:36170/148‑1 (MQ=255)            ggcTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGa                                                                                                                                 >  6:206845/1‑145 (MQ=255)            ggcTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGa                                                                                                                                 <  5:206845/145‑1 (MQ=255)               tcgctcAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGa                                                                                                                          >  7:234822/1‑149 (MQ=255)               tcgctcAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGa                                                                                                                          >  7:155671/1‑149 (MQ=255)                                    aCCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGaaaaa                                                                                                     >  1:193232/1‑149 (MQ=255)                                    aCCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGaaaaa                                                                                                     <  2:268696/149‑1 (MQ=255)                                     ccGGGCGTAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAAc                                                                                                    <  8:118596/149‑1 (MQ=255)                                          cgcAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCaa                                                                                               <  8:155671/149‑1 (MQ=255)                                             aaCATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGcc                                                                                            >  8:224845/1‑149 (MQ=255)                                             aaCATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGcc                                                                                            >  6:31399/1‑149 (MQ=255)                                                aTGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGct                                                                                         <  2:193232/149‑1 (MQ=255)                                                 tGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACACC‑ACGTCGGGGTTGAgga                                                                                                                              <  1:167573/111‑3 (MQ=255)                                                 tGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACACC‑ACGTCGGGGTTGAgga                                                                                                                              >  2:167573/1‑109 (MQ=255)                                                        cTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTgccg                                                                                 >  7:189295/1‑149 (MQ=255)                                                            gTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGa                                                                             <  4:26178/149‑1 (MQ=255)                                                                    ggAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCCCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCAcc                                                                                                            <  4:92379/110‑1 (MQ=255)                                                                    ggAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCCCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCAcc                                                                                                            <  4:96866/110‑1 (MQ=255)                                                                    ggAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCCCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCAcc                                                                                                            >  3:92379/1‑110 (MQ=255)                                                                    ggAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCCCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCAcc                                                                                                            >  3:96866/1‑110 (MQ=255)                                                                     gAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAAtcgt                                                                    <  7:224845/149‑1 (MQ=255)                                                                         gggggCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGa                                                                             >  8:238192/1‑136 (MQ=255)                                                                         gggggCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGa                                                                             <  7:238192/136‑1 (MQ=255)                                                                           gggCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCAc                                                                                                             >  8:297092/1‑102 (MQ=255)                                                                           gggCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCAc                                                                                                             <  7:297092/102‑1 (MQ=255)                                                                           gggCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGAc                                                                >  6:105146/1‑147 (MQ=255)                                                                             gCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAAtcgtc                                                                   <  8:260501/142‑1 (MQ=255)                                                                             gCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAAtcgtc                                                                   >  7:260501/1‑142 (MQ=255)                                                                                     acTAGCAGCTGATGTGGAATCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCTGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAg                                                    <  8:288372/148‑1 (MQ=255)                                                                                      cTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGt                                                   <  8:189295/149‑1 (MQ=255)                                                                                         ccAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGc                                                 <  5:31399/148‑1 (MQ=255)                                                                                             cTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCt                                             >  1:109374/1‑148 (MQ=255)                                                                                                  gtgGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGc                                        >  4:213531/1‑148 (MQ=255)                                                                                                   tgGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCg                                       >  7:87892/1‑148 (MQ=255)                                                                                                     gAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATc                                                       <  6:321421/130‑1 (MQ=255)                                                                                                     gAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATc                                                       <  8:160012/130‑1 (MQ=255)                                                                                                     gAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAAc                                    >  4:134673/1‑149 (MQ=255)                                                                                                          tCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGgtg                               >  5:50668/1‑149 (MQ=255)                                                                                                                     cTCGACGCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTcc                      >  7:255737/1‑147 (MQ=255)                                                                                                                        gACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGtgtg                             <  6:98508/137‑1 (MQ=255)                                                                                                                        gACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGtgtg                             >  5:98508/1‑137 (MQ=255)                                                                                                                         aCCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAACCCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGc                <  8:234822/149‑1 (MQ=255)                                                                                                                          ccccAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGcc               >  7:183205/1‑149 (MQ=255)                                                                                                                           cccAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCt              >  7:260882/1‑149 (MQ=255)                                                                                                                             cAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGc                >  8:111560/1‑145 (MQ=255)                                                                                                                             cAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGc                <  7:111560/145‑1 (MQ=255)                                                                                                                              aaCGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGAc           >  5:8711/1‑149 (MQ=255)                                                                                                                                  gCATCCTCACCCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCAGCGAACCGGTGTGCCCGTCCAAGGGCCTGCCCCTc       >  2:246663/1‑149 (MQ=255)                                                                                                                                  gCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTAAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGCTGTGCCCGTCCAAGGGCCTGACCCt        >  2:191350/1‑148 (MQ=255)                                                                                                                                     tCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGc    <  3:134673/149‑1 (MQ=255)                                                                                                                                       cTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGccc  >  4:189397/1‑149 (MQ=255)                                                                                                                                        |                                                                                                                                                     TGGAGTTTGGCGGCTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGT‑GGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCC  >  NC_002947/5387884‑5388166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑