Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,416,662	G→A	9.3%	S341F (TCT→TTT)	gudD ←	bifunctional D‑glucarate dehydratase/L‑idarate epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,416,662	0	G	A	9.3%	118.6 / 2.3	43	S341F (TCT→TTT)	gudD	bifunctional D‑glucarate dehydratase/L‑idarate epimerase
Reads supporting (aligned to +/- strand):  ref base G (19/20);  new base A (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

CCGTCGACAATGCGCAGCGGCTCACGGGTCAGGCGCTGGCCATCCTGCCAGATCCAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAACTGAATGGCATGGCCCATCTGTCGCCAGTCGGTAGCGATC  >  NC_002947/5416515‑5416805                                                                                                                                                     |                                                                                                                                                ccGTCGACAATGCGCAGAGTCTAACGGGTCAGGCTCTGGCCATCCTGCCAGATCCAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGTGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATgag                                                                                                                                                 <  5:209201/148‑1 (MQ=255)   gTCGACAATGCGCAGCGGCTCACGGGTCAGGCGCTGGCCATCCTGCCAGATCCAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAAc                                                                                                                                              >  3:110362/1‑149 (MQ=255)      gACAATGCGCAGCGGCTCACGGGTCAGGCGCTGGCCATCCTGCCAGATCCAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAAcccc                                                                                                                                           <  2:273852/149‑1 (MQ=255)            gcgcAGCGGCTCACGGGTCAGGCGCTGGCCATCCTGCCAGATCCAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGt                                                                                                                                       >  5:286786/1‑147 (MQ=255)                        aCGGGTCAGGCGCTGGCCATCCTGCCAGATCCAGTGGGTATCGATCGCAGTGAAGTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCg                                                                                                                          >  1:86878/1‑149 (MQ=255)                           ggTCAGGCGCTGGCCATCCTGCCAGATCCAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGc                                                                                                                      <  6:175441/149‑1 (MQ=255)                            gTCAGGCGCTGGCCATCCTGCCAGATCCAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGca                                                                                                                     >  4:290445/1‑149 (MQ=255)                            gTCAGGCGCTGGCCATCCTGCCAGATCCAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCACAGGTCAGCCCCCAGTCGTTGca                                                                                                                     >  3:95345/1‑149 (MQ=255)                                          aTCCTGCCAGATCCAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATATGCGCCAccc                                                                                                       >  6:317943/1‑149 (MQ=255)                                                   gATCCAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCcgaaccgaac                                                                                              >  6:157222/1‑149 (MQ=255)                                                      ccAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCt                                                                                           >  6:64871/1‑149 (MQ=255)                                                        aGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTg                                                                                          >  6:318741/1‑148 (MQ=255)                                                                tcgatcgCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTcc                                                                                  <  5:244971/148‑1 (MQ=255)                                                                tcgatcgCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTcc                                                                                  <  3:290445/148‑1 (MQ=255)                                                                   atcgCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGTTCAGACCCCAGTCGTTGCACATCTGCGCCAAACGAACAGAACCCTGCAAgttacata                                                                               >  2:112583/1‑141 (MQ=255)                                                                      ggaGTG‑ATTTCACCCGGCGCCGCTGACGCCTCCTGGTTGAACATCGCCAGCGAAATGTACAAGTGGTTATTGGAATGAGAACTCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTACAGAAGt                                                                            <  6:321705/146‑1 (MQ=255)                                                                       cAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGt                                                                            >  3:60477/1‑147 (MQ=255)                                                                       cAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGa                                                                          <  4:60477/149‑1 (MQ=255)                                                                          tG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAggg                                                                       <  6:107324/149‑1 (MQ=255)                                                                               ttCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGGAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCgg                                                                   >  1:69528/1‑149 (MQ=255)                                                                                 cACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGc                                                                  <  5:318741/148‑1 (MQ=255)                                                                                  aCCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAAAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGc                                                                  >  6:227154/1‑147 (MQ=255)                                                                                  aCCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAAAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGc                                                                  >  6:52811/1‑147 (MQ=255)                                                                                    ccGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGc                                                              <  4:110362/149‑1 (MQ=255)                                                                                    ccGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGc                                                              <  5:64871/149‑1 (MQ=255)                                                                                         gccgcAGCCGTCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAAt                                                         <  1:112583/149‑1 (MQ=255)                                                                                          ccgcAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAAt                                                         >  4:304514/1‑148 (MQ=255)                                                                                          ccgcAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAAt                                                         <  3:304514/148‑1 (MQ=255)                                                                                               gccgccACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCAc                                                   <  6:286786/149‑1 (MQ=255)                                                                                                      ccTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGGCAGCGGATTGTCCACCGACTg                                             >  6:76300/1‑148 (MQ=255)                                                                                                         gggTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAAc                                         >  2:312810/1‑149 (MQ=255)                                                                                                          ggTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAAc                                         <  5:301850/148‑1 (MQ=255)                                                                                                                    gCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAACTGAatggcatg                              <  2:69528/149‑1 (MQ=255)                                                                                                                     ccAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGCACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAAAGTCCACCGACTGTAACTGAatggcatgg                             >  3:243158/1‑149 (MQ=255)                                                                                                                      cAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGTTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAACTGAatggcatggc                            <  2:86878/149‑1 (MQ=255)                                                                                                                                 cGAAGTGGTTATTTGAATGAGCACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGGGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAACTGAATGGCATGGCCCATCTGTCGc                 <  2:166776/149‑1 (MQ=255)                                                                                                                                  gAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAACTGaa                                     >  2:324230/1‑128 (MQ=255)                                                                                                                                  gAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAACTGaa                                     <  1:324230/128‑1 (MQ=255)                                                                                                                                   aaGTGGTTATTGGAATGAAAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAACTGAATGGCATGGCCCATCTGTCGCCa               <  5:52811/149‑1 (MQ=255)                                                                                                                                   aaGTGGTTATTGGAATGAAAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAACTGAATGGCATGGCCCATCTGTCGCCa               <  5:227154/149‑1 (MQ=255)                                                                                                                                           aTTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAACTGAATGGCATGGCCCATCTGTCGCCAGTCGGTAg       >  1:157972/1‑149 (MQ=255)                                                                                                                                                aaTGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAACTGAATGGCATGGCCCATCTGTCGCCAGTCGGTAGCGATc  <  7:214533/149‑1 (MQ=255)                                                                                                                                                  tGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGATGGTCGGCCAGCGGAATGTCCACCGa                                                <  7:161107/101‑1 (MQ=255)                                                                                                                                                  tGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGa                                                >  8:161107/1‑101 (MQ=255)                                                                                                                                                     |                                                                                                                                                CCGTCGACAATGCGCAGCGGCTCACGGGTCAGGCGCTGGCCATCCTGCCAGATCCAGTGGGTATCGATCGCAGTG‑ATTTCACCCGGCGCCGCAGCCGCCACCTGGGTGAACATCGCCAGCGAAATGTCGAAGTGGTTATTGGAATGAGAACCCCAGGTCAGCCCCCAGTCGTTGCACATCTGCGCCACCCGAACCGAACCCTGCAAGGTCCAGAAGTGAGGGTCGGCCAGCGGAATGTCCACCGACTGTAACTGAATGGCATGGCCCATCTGTCGCCAGTCGGTAGCGATC  >  NC_002947/5416515‑5416805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑