Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,447,367 C→T 6.5% P291L (CCG→CTG)  hemL → glutamate‑1‑semialdehyde 2,1‑aminomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,447,3670CT 6.5% 181.9 / 2.3 61P291L (CCG→CTG) hemLglutamate‑1‑semialdehyde 2,1‑aminomutase
Reads supporting (aligned to +/- strand):  ref base C (28/29);  new base T (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

GTTTCCGCGTCTCGCTCGGCGGCGCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTGCAGCAACGCGCC  >  NC_002947/5447220‑5447512
                                                                                                                                                   |                                                                                                                                                 
gTTTCCGCGTCTCGCTCGGCGGCGCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCg                                                                                                                                                  >  3:249890/1‑149 (MQ=255)
gTTTCCGCGTCTCGCTCGGCGGCGCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCg                                                                                                                                                  <  5:55927/149‑1 (MQ=255)
             gctcgGCGGCGCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCt                                                                                                                                              <  5:226761/140‑1 (MQ=255)
             gctcgGCGGCGCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCt                                                                                                                                              >  6:226761/1‑140 (MQ=255)
                  gcggcgCCCAGGGCCATTACGGCATCAAGCGGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCAcc                                                                                                                                <  1:150423/149‑1 (MQ=255)
                  gcggcgCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCAcc                                                                                                                                <  4:292268/149‑1 (MQ=255)
                       gcCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCg                                                                                                                                                  >  6:165271/1‑126 (MQ=255)
                       gcCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCg                                                                                                                                                  <  5:165271/126‑1 (MQ=255)
                        cccAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCg                                                                                                                          <  1:157131/149‑1 (MQ=255)
                         ccAGGGCCATTACGGCATCAAGCCGGACCTGTCGCCCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCgg                                                                                                                         >  5:3758/1‑149 (MQ=255)
                         ccAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCgg                                                                                                                         <  2:67998/149‑1 (MQ=255)
                           aGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGc                                                                                                                       <  5:102204/149‑1 (MQ=255)
                               ccATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAAcc                                                                                                                   >  3:21654/1‑149 (MQ=255)
                               ccATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAAc                                                                                                                    >  3:193187/1‑148 (MQ=255)
                               ccATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAAc                                                                                                                    <  4:193187/148‑1 (MQ=255)
                                  ttACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCgg                                                                                                                         <  1:269636/140‑1 (MQ=255)
                                  ttACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCgg                                                                                                                         >  2:269636/1‑140 (MQ=255)
                                   tACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCTGGTAGACCGGGCCgagcgg                                                                                                                                <  6:334567/132‑6 (MQ=255)
                                   tACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCTGGTAGACCGGGCCgagcgg                                                                                                                                >  5:334567/1‑127 (MQ=255)
                                         aTCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTGGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCAt                                                                                                         <  4:295835/149‑1 (MQ=255)
                                          tCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATg                                                                                                        <  5:768/149‑1 (MQ=255)
                                              gCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGgccg                                                                                                    >  5:190468/1‑149 (MQ=255)
                                                 ggACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGccgccg                                                                                                 <  8:91909/149‑1 (MQ=255)
                                                       gTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAGCCCGCTGGCCATGGCCGCCGGCCTGa                                                                                           <  4:249890/149‑1 (MQ=255)
                                                        tCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGa                                                                                           <  6:190468/148‑1 (MQ=255)
                                                          gACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGacca                                                                                        <  6:3758/149‑1 (MQ=255)
                                                             cTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGaccacc                                                                                      >  6:50918/1‑148 (MQ=255)
                                                              ttCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGACGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCt                                                                                    >  6:161114/1‑149 (MQ=255)
                                                                    aaGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCt                                                                              >  2:119019/1‑149 (MQ=255)
                                                                      gATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGa                                                                            <  7:56632/149‑1 (MQ=255)
                                                                          gtcgGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCa                                                                         <  5:50918/148‑1 (MQ=255)
                                                                                 gcggcATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGCCCACCCTCAAGCTGATCAgccgcc                                                                   >  6:323850/1‑147 (MQ=255)
                                                                                 gcggcATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCa                                                                         >  8:235303/1‑141 (MQ=255)
                                                                                 gcggcATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCa                                                                         <  7:235303/141‑1 (MQ=255)
                                                                                 gcggcATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCa                                                                 <  1:216257/149‑1 (MQ=255)
                                                                                      aTGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCTGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCtt                                                            <  1:205190/149‑1 (MQ=255)
                                                                                        gCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGcata                                                           >  5:216434/1‑145 (MQ=255)
                                                                                        cccGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCt                                                             <  6:216434/145‑1 (MQ=255)
                                                                                                                 cGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAg                                 <  4:21654/149‑1 (MQ=255)
                                                                                                                    gAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCg                              >  6:159199/1‑149 (MQ=255)
                                                                                                                    gAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCg                              >  2:113936/1‑149 (MQ=255)
                                                                                                                     aaaTCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGcc                               >  5:293948/1‑147 (MQ=255)
                                                                                                                     aaaTCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGcc                               <  6:293948/147‑1 (MQ=255)
                                                                                                                     aaaTCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCg                              <  5:161114/148‑1 (MQ=255)
                                                                                                                       aTCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCAt                           >  5:84870/1‑149 (MQ=255)
                                                                                                                        tCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATg                          >  7:291442/1‑149 (MQ=255)
                                                                                                                           tGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCtt                       >  4:143533/1‑149 (MQ=255)
                                                                                                                             ggCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGa                     >  5:44967/1‑149 (MQ=255)
                                                                                                                              gCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTgcc                    >  7:236563/1‑147 (MQ=255)
                                                                                                                              gCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGAc                    <  1:119019/149‑1 (MQ=255)
                                                                                                                               cTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTacac                                    >  2:117912/1‑132 (MQ=255)
                                                                                                                                tGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCa                                  <  7:137064/133‑1 (MQ=255)
                                                                                                                                tGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCa                                  >  8:137064/1‑133 (MQ=255)
                                                                                                                                     cGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTg              >  7:267214/1‑148 (MQ=255)
                                                                                                                                      gCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCCCCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTg              >  2:88287/1‑147 (MQ=255)
                                                                                                                                      gCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTgc             <  8:267214/148‑1 (MQ=255)
                                                                                                                                         ccGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTgcagc          >  3:100050/1‑148 (MQ=255)
                                                                                                                                            cTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTGCAGCAAcg      >  6:224028/1‑149 (MQ=255)
                                                                                                                                              cGGCCTGGTAGACCGGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTgc             >  7:118405/1‑140 (MQ=255)
                                                                                                                                              cGGCCTGGTAGACCGGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTgc             <  8:118405/140‑1 (MQ=255)
                                                                                                                                                gCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTGCAGCAACGCGcc  <  4:289819/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTTTCCGCGTCTCGCTCGGCGGCGCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTGCAGCAACGCGCC  >  NC_002947/5447220‑5447512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑