Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,447,371 C→A 7.0% V292V (GTC→GTA)  hemL → glutamate‑1‑semialdehyde 2,1‑aminomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,447,3710CA 7.0% 181.7 / 3.0 57V292V (GTC→GTA) hemLglutamate‑1‑semialdehyde 2,1‑aminomutase
Reads supporting (aligned to +/- strand):  ref base C (26/27);  new base A (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

GCTCGGCGGCGCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTGCAGCAACGCGCC  >  NC_002947/5447233‑5447512
                                                                                                                                          |                                                                                                                                             
gctcgGCGGCGCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCt                                                                                                                                              >  6:226761/1‑140 (MQ=255)
gctcgGCGGCGCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCt                                                                                                                                              <  5:226761/140‑1 (MQ=255)
     gcggcgCCCAGGGCCATTACGGCATCAAGCGGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCAcc                                                                                                                                <  1:150423/149‑1 (MQ=255)
     gcggcgCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCAcc                                                                                                                                <  4:292268/149‑1 (MQ=255)
           cccAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCg                                                                                                                          <  1:157131/149‑1 (MQ=255)
            ccAGGGCCATTACGGCATCAAGCCGGACCTGTCGCCCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCgg                                                                                                                         >  5:3758/1‑149 (MQ=255)
            ccAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCgg                                                                                                                         <  2:67998/149‑1 (MQ=255)
              aGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGc                                                                                                                       <  5:102204/149‑1 (MQ=255)
                  ccATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAAcc                                                                                                                   >  3:21654/1‑149 (MQ=255)
                  ccATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAAc                                                                                                                    <  4:193187/148‑1 (MQ=255)
                  ccATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAAc                                                                                                                    >  3:193187/1‑148 (MQ=255)
                     ttACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCgg                                                                                                                         <  1:269636/140‑1 (MQ=255)
                     ttACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCgg                                                                                                                         >  2:269636/1‑140 (MQ=255)
                      tACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCTGGTAGACCGGGCCgagcgg                                                                                                                                >  5:334567/1‑127 (MQ=255)
                      tACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCTGGTAGACCGGGCCgagcgg                                                                                                                                <  6:334567/132‑6 (MQ=255)
                            aTCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTGGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCAt                                                                                                         <  4:295835/149‑1 (MQ=255)
                             tCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATg                                                                                                        <  5:768/149‑1 (MQ=255)
                                 gCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGgccg                                                                                                    >  5:190468/1‑149 (MQ=255)
                                    ggACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGccgccg                                                                                                 <  8:91909/149‑1 (MQ=255)
                                          gTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAGCCCGCTGGCCATGGCCGCCGGCCTGa                                                                                           <  4:249890/149‑1 (MQ=255)
                                           tCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGa                                                                                           <  6:190468/148‑1 (MQ=255)
                                             gACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGacca                                                                                        <  6:3758/149‑1 (MQ=255)
                                                cTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGaccacc                                                                                      >  6:50918/1‑148 (MQ=255)
                                                 ttCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGACGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCt                                                                                    >  6:161114/1‑149 (MQ=255)
                                                       aaGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCt                                                                              >  2:119019/1‑149 (MQ=255)
                                                         gATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGa                                                                            <  7:56632/149‑1 (MQ=255)
                                                             gtcgGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCa                                                                         <  5:50918/148‑1 (MQ=255)
                                                                    gcggcATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGCCCACCCTCAAGCTGATCAgccgcc                                                                   >  6:323850/1‑147 (MQ=255)
                                                                    gcggcATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCa                                                                         >  8:235303/1‑141 (MQ=255)
                                                                    gcggcATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCa                                                                         <  7:235303/141‑1 (MQ=255)
                                                                    gcggcATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCa                                                                 <  1:216257/149‑1 (MQ=255)
                                                                         aTGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCTGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCtt                                                            <  1:205190/149‑1 (MQ=255)
                                                                           gCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGcata                                                           >  5:216434/1‑145 (MQ=255)
                                                                           cccGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCt                                                             <  6:216434/145‑1 (MQ=255)
                                                                                                    cGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAg                                 <  4:21654/149‑1 (MQ=255)
                                                                                                       gAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCg                              >  6:159199/1‑149 (MQ=255)
                                                                                                       gAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCg                              >  2:113936/1‑149 (MQ=255)
                                                                                                        aaaTCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGcc                               <  6:293948/147‑1 (MQ=255)
                                                                                                        aaaTCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGcc                               >  5:293948/1‑147 (MQ=255)
                                                                                                        aaaTCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCg                              <  5:161114/148‑1 (MQ=255)
                                                                                                          aTCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCAt                           >  5:84870/1‑149 (MQ=255)
                                                                                                           tCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATg                          >  7:291442/1‑149 (MQ=255)
                                                                                                              tGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCtt                       >  4:143533/1‑149 (MQ=255)
                                                                                                                ggCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGa                     >  5:44967/1‑149 (MQ=255)
                                                                                                                 gCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTgcc                    >  7:236563/1‑147 (MQ=255)
                                                                                                                 gCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGAc                    <  1:119019/149‑1 (MQ=255)
                                                                                                                  cTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTacac                                    >  2:117912/1‑132 (MQ=255)
                                                                                                                   tGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCa                                  <  7:137064/133‑1 (MQ=255)
                                                                                                                   tGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCa                                  >  8:137064/1‑133 (MQ=255)
                                                                                                                        cGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTg              >  7:267214/1‑148 (MQ=255)
                                                                                                                         gCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCCCCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTg              >  2:88287/1‑147 (MQ=255)
                                                                                                                         gCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTgc             <  8:267214/148‑1 (MQ=255)
                                                                                                                            ccGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTgcagc          >  3:100050/1‑148 (MQ=255)
                                                                                                                               cTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTGCAGCAAcg      >  6:224028/1‑149 (MQ=255)
                                                                                                                                 cGGCCTGGTAGACCGGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTgc             >  7:118405/1‑140 (MQ=255)
                                                                                                                                 cGGCCTGGTAGACCGGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTgc             <  8:118405/140‑1 (MQ=255)
                                                                                                                                   gCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTGCAGCAACGCGcc  <  4:289819/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                             
GCTCGGCGGCGCCCAGGGCCATTACGGCATCAAGCCGGACCTGTCGACCTTCGGCAAGATCGTCGGTGGCGGCATGCCGGTCGGCTGTTTCGGCGGCAAGCGTGAAATCATGGGCTGCATCGCCCCGCTCGGCCCGGTCTACCAGGCCGGCACCCTGTCGGGCAACCCGCTGGCCATGGCCGCCGGCCTGACCACCCTCAAGCTGATCAGCCGCCCAGGCTTCCACGCCGAGCTGACCGACTACACCAGCCGCATGCTTGACGGCCTGCAGCAACGCGCC  >  NC_002947/5447233‑5447512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑