Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,452,102	G→C	8.3%	A211A (GCG→GCC)	lnt →	apolipoprotein N‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,452,102	0	G	C	8.3%	143.5 / 4.6	48	A211A (GCG→GCC)	lnt	apolipoprotein N‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base C (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

GCCACTGGGCGGGGTATGGCTGATTTCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGG  >  NC_002947/5451958‑5452249                                                                                                                                                  |                                                                                                                                                      gcCACTGGGCGGGGTATGGCTGATTTCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTc                                                                                                                                                    >  1:148120/1‑148 (MQ=255) gcCACTGGGCGGGGTATGGCTGATATCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCA‑TGGCCCttg                                                                                                                                                   >  7:194602/1‑147 (MQ=255)    aCTGGGCGGGGTATGGCTGATTTCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAgg                                                                                                                                                >  8:172723/1‑149 (MQ=255)        ggCGGGGTATGGCTGATTTCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCa                                                                                                                                            >  2:205166/1‑149 (MQ=255)        ggCGGGGTATGGCTGATTTCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCAGGGGGCTGGGCC‑TGGCGCTCAAGGGc                                                                                                                                              <  4:63905/147‑1 (MQ=255)          cGGGGTATGGCTGATTTCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGG‑CCCCTGGGGGCTGGGCC‑TGGCCCTTGAGCGCCAGGc                                                                                                                                         <  5:203076/149‑1 (MQ=255)           ggggTATGGCTGATTTCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGc                                                                                                                                         <  2:148120/149‑1 (MQ=255)              gTATGGCTGATATCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCA‑TGGCCCTTGAGCGCCAGGccca                                                                                                                                      <  8:194602/149‑3 (MQ=255)                 tGGCTGATTTCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGAc                                                                                                                                   <  5:145672/149‑1 (MQ=255)                        tttCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCg                                                                                                                            <  5:173108/149‑1 (MQ=255)                                aCCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCACCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTTCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGa                                                                                                                    >  2:247796/1‑149 (MQ=255)                                    tCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCg                                                                                                                            <  8:37503/137‑1 (MQ=255)                                    tCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTACCTCGCCGTGGCCAGCGTGCAGTTGCTGGCCACCTGGGGGCTGGGCC‑TGGCGCTCAAGGGACATGCCTGGACCATTCCGGCTGGCGATCCg                                                                                                                >  1:173532/1‑149 (MQ=255)                                            aCCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCGCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑aaa                                                                                                         <  3:38511/147‑1 (MQ=255)                                              cgccgcCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTgg                                                                                                     <  3:274855/149‑1 (MQ=255)                                                          gtgCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAg                                                                                                        >  7:101921/1‑134 (MQ=255)                                                          gtgCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAg                                                                                                        <  8:101921/134‑1 (MQ=255)                                                               aCCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑Ac                                                                                   <  1:205166/149‑1 (MQ=255)                                                                ccTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACg                                                                                  >  8:149198/1‑149 (MQ=255)                                                                       cGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAg                                                                             >  1:173010/1‑147 (MQ=255)                                                                                    gccccTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATgg                                                              <  7:149198/149‑1 (MQ=255)                                                                                      cccTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGa                                                            <  2:201257/149‑1 (MQ=255)                                                                                        cTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCCAGGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGAc                                                           >  1:251320/1‑149 (MQ=255)                                                                                               cTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGca                                                   >  6:125624/1‑149 (MQ=255)                                                                                                tGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGca                                                   >  4:256457/1‑148 (MQ=255)                                                                                                   ccGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATc                                               >  7:180305/1‑149 (MQ=255)                                                                                                        gCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGAcgc                                          >  2:267661/1‑149 (MQ=255)                                                                                                                 cTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCATTCGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTgg                                  <  1:247796/149‑1 (MQ=255)                                                                                                                 cTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTgg                                  <  7:172723/148‑1 (MQ=255)                                                                                                                  tGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGgcg                                >  3:38453/1‑149 (MQ=255)                                                                                                                   gTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGgcgc                               >  6:112936/1‑149 (MQ=255)                                                                                                                        tGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTgg                                  <  3:28866/141‑1 (MQ=255)                                                                                                                        tGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTgg                                  >  4:28866/1‑141 (MQ=255)                                                                                                                         ggCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGGCGCTATAcc                         >  2:196763/1‑149 (MQ=255)                                                                                                                         ggCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGGCGCTATAcc                         >  3:145559/1‑149 (MQ=255)                                                                                                                          gCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGAcc                                                                      >  3:303070/1‑103 (MQ=255)                                                                                                                          gCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGAcc                                                                      <  4:303070/103‑1 (MQ=255)                                                                                                                           cccccTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGGCGCTATACcgc                       <  4:145559/149‑1 (MQ=255)                                                                                                                           ccacgtGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGGCGCTATACcgc                       <  3:252828/144‑1 (MQ=255)                                                                                                                            ccccTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGGCGCTATACcgcg                      >  6:313055/1‑149 (MQ=255)                                                                                                                             cccTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACgcgc                                        <  5:277181/130‑1 (MQ=255)                                                                                                                             cccTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACgcgc                                        >  6:277181/1‑130 (MQ=255)                                                                                                                              ccTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGGCGCTATACCGCGAc                    <  5:125624/149‑1 (MQ=255)                                                                                                                              ccTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGGCGCTATACCGCGAc                    <  2:173010/149‑1 (MQ=255)                                                                                                                                  ggggCTGGGCC‑TGGCGCTCAAGGGCCATGGCTGGAGCTTTGCGGCTGGCGATCCGGTG‑ATATTGGCAGCGGTCGAGGGCA‑ACGTCGAGCAGGACCTGAAATGTGACCCCGCGCACATCGACGCGCAACTGGCGCTATTCCGCGACCTGa                <  2:173532/149‑1 (MQ=255)                                                                                                                                    gtccaggCA‑TGGCCCTTGAGCGCCAGGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGGCGCTATACCGCGACCTGAGc              >  2:234333/6‑149 (MQ=255)                                                                                                                                      cTGGGCC‑TGGCGCTCAAGGTCCATGCCTGGACGATTCCGGCAGTCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCAGATCGACGCGCAACTGGCGCTATACCGCGACCTGAGCt             <  3:23212/148‑1 (MQ=255)                                                                                                                                         ggCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTC‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGAACAGAAATCGGACCCCGCGCACATCGACGCGCAACTGGAGCTATACCGCGACCTGAGAttag          >  4:23212/1‑146 (MQ=255)                                                                                                                                                  gcTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTGAAAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAgg  >  1:69419/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                      GCCACTGGGCGGGGTATGGCTGATTTCCTTCACCCTCGCCCTTACCGCCGCCCTGCTGTGCAACCTGCACCGCCTGCGCCCCCGCCCCTCGTTCCTGGCCGTGGCCAGCGTGCTGTTGCTGGCCCCCTGGGGGCTGGGCC‑TGGCGCTCAAGGGCCATGCCTGGACCATTCCGGCAGGCGATCCGCTG‑AAAGTGGCAGCGATCCAGGGCA‑ACGTCGAGCAGGACCTGAAATGGGACCCCGCGCACATCGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGG  >  NC_002947/5451958‑5452249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑