Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,469,180 A→T 8.8% L273H (CTC→CAC)  mrdB ← murein transpeptidase factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,469,1800AT 8.8% 159.2 / 5.7 57L273H (CTC→CAC) mrdBmurein transpeptidase factor
Reads supporting (aligned to +/- strand):  ref base A (31/21);  new base T (2/3);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

GTATACAAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAA  >  NC_002947/5469032‑5469319
                                                                                                                                                    |                                                                                                                                           
gTATACAAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGTTCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGa                                                                                                                                             <  1:260108/149‑1 (MQ=255)
    aCAAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCAc                                                                                                                                         >  5:255493/1‑149 (MQ=255)
      aaaaaaaGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACgg                                                                                                                                       >  7:217784/1‑149 (MQ=255)
           aaGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgagg                                                                                                                                  <  2:248944/149‑3 (MQ=255)
            aGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgagga                                                                                                                                 <  8:57400/149‑1 (MQ=255)
            aGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgagga                                                                                                                                 <  4:159581/149‑1 (MQ=255)
            aGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCgatga                                                                                                                                 <  6:55728/149‑1 (MQ=255)
                   gTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGc                                                                                                                                           >  8:114755/1‑132 (MQ=255)
                   gTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGc                                                                                                                                           >  8:119310/1‑132 (MQ=255)
                   gTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTc                                                                                                                          >  8:167626/1‑149 (MQ=255)
                    tCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCg                                                                                                                         <  7:3776/149‑1 (MQ=255)
                      aGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGgt                                                                                                                       <  6:255493/149‑1 (MQ=255)
                       ggCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCTGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTATACAATCAGCAGGAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGgtg                                                                                                                      <  6:334032/149‑1 (MQ=255)
                             ccGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCtt                                                                                                                >  6:281171/1‑149 (MQ=255)
                                          gCCGAACAGGGTCTGCGCCTGGGCGGTGCTCCCCCGGCCACGGCCGATCCGCAGCAGGTAGACAAGCAGCAGCAGGCAGCTGCCTCCCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCACAAAGt                                                                                                   >  7:122242/1‑149 (MQ=255)
                                            cGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGGCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTcc                                                                                                 <  5:281171/149‑1 (MQ=255)
                                             gAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTc                                                                                                  >  3:183319/1‑147 (MQ=255)
                                                    gTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGa                                                                                         >  6:99840/1‑149 (MQ=255)
                                                           ccTGGGCGGTGATCACCAGGCCACGGCCGAGCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTg                                                                                  <  4:85810/149‑1 (MQ=255)
                                                                  ggTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCt                                                                                                                 >  4:181196/1‑111 (MQ=255)
                                                                  ggTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCt                                                                                                                 <  3:181196/111‑1 (MQ=255)
                                                                  ggTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAag                                                                             >  2:179481/1‑147 (MQ=255)
                                                                    tGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAagcagc                                                                         <  1:179481/149‑1 (MQ=255)
                                                                      aTCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTcc                                                                                                 <  6:181676/123‑1 (MQ=255)
                                                                      aTCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTcc                                                                                                 >  5:181676/1‑123 (MQ=255)
                                                                         aCCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACCGCGATGATGAAGTCGGTGTGCCTTTCCGGCAAAACGTCCAGGTGCGACTGCGTGCAAAGCagccagcc                                                                    >  8:116094/1‑149 (MQ=255)
                                                                          ccAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGTCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCGAAGCAGCCAGGCt                                                                   >  8:43089/1‑149 (MQ=255)
                                                                           cAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCtt                                                                  <  1:67492/149‑1 (MQ=255)
                                                                           cAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCtt                                                                  >  4:262101/1‑149 (MQ=255)
                                                                            aGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCttt                                                                 <  5:99840/149‑1 (MQ=255)
                                                                            aGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCttt                                                                 <  8:217784/149‑1 (MQ=255)
                                                                                  cGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCaaa                                                           <  4:183319/149‑1 (MQ=255)
                                                                                            gcagcagGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              >  6:41108/1‑120 (MQ=255)
                                                                                            gcagcagGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              <  5:41108/120‑1 (MQ=255)
                                                                                                 agGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGcc                                            >  3:130098/1‑149 (MQ=255)
                                                                                                   gTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGa                                          >  1:212962/1‑149 (MQ=255)
                                                                                                      gACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCttt                                                                 <  8:287081/123‑1 (MQ=255)
                                                                                                         aaTCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATcgccg                                    >  2:81420/1‑149 (MQ=255)
                                                                                                           tCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCgccgcc                                  >  3:188315/1‑149 (MQ=255)
                                                                                                            cagcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              <  4:270815/104‑1 (MQ=255)
                                                                                                            cagcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              >  3:270815/1‑104 (MQ=255)
                                                                                                             agcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCtt                                >  5:255813/1‑149 (MQ=255)
                                                                                                              gcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCtt                                >  3:19758/1‑148 (MQ=255)
                                                                                                              gcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCtt                                <  4:19758/148‑1 (MQ=255)
                                                                                                                     gcagATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAg                                                                                               >  6:94034/1‑78 (MQ=255)
                                                                                                                     gcagATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAg                                                                                               <  5:94034/78‑1 (MQ=255)
                                                                                                                     gcagATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGga                        >  4:114809/1‑149 (MQ=255)
                                                                                                                       agATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGgat                       >  2:240469/1‑148 (MQ=255)
                                                                                                                             cTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTc                >  6:73042/1‑149 (MQ=255)
                                                                                                                               aCCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTcc               >  1:253905/1‑148 (MQ=255)
                                                                                                                                 cAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAAc            <  4:130098/149‑1 (MQ=255)
                                                                                                                                   ggCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCg          >  7:103901/1‑149 (MQ=255)
                                                                                                                                      cGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTg       <  4:188315/149‑1 (MQ=255)
                                                                                                                                       ttcatcatCGCCGTGCTCGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGc      >  3:254104/8‑149 (MQ=255)
                                                                                                                                         aTTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACAGGTGcc     >  3:33977/1‑148 (MQ=255)
                                                                                                                                           tcatCGCCGTGCTCGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCaa  >  1:212847/4‑149 (MQ=255)
                                                                                                                                              tCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              >  3:267822/1‑70 (MQ=255)
                                                                                                                                              tCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              <  4:267822/70‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
GTATACAAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAA  >  NC_002947/5469032‑5469319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑