Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,469,183 A→T 9.0% V272E (GTG→GAG)  mrdB ← murein transpeptidase factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,469,1830AT 9.0% 146.7 / 3.3 55V272E (GTG→GAG) mrdBmurein transpeptidase factor
Reads supporting (aligned to +/- strand):  ref base A (29/21);  new base T (2/3);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAA  >  NC_002947/5469036‑5469319
                                                                                                                                                   |                                                                                                                                        
aCAAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCAc                                                                                                                                         >  5:255493/1‑149 (MQ=255)
  aaaaaaaGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACgg                                                                                                                                       >  7:217784/1‑149 (MQ=255)
       aaGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgagg                                                                                                                                  <  2:248944/149‑3 (MQ=255)
        aGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgagga                                                                                                                                 <  8:57400/149‑1 (MQ=255)
        aGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgagga                                                                                                                                 <  4:159581/149‑1 (MQ=255)
        aGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCgatga                                                                                                                                 <  6:55728/149‑1 (MQ=255)
               gTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTc                                                                                                                          >  8:167626/1‑149 (MQ=255)
                tCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCg                                                                                                                         <  7:3776/149‑1 (MQ=255)
                  aGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGgt                                                                                                                       <  6:255493/149‑1 (MQ=255)
                   ggCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCTGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTATACAATCAGCAGGAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGgtg                                                                                                                      <  6:334032/149‑1 (MQ=255)
                         ccGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCtt                                                                                                                >  6:281171/1‑149 (MQ=255)
                                      gCCGAACAGGGTCTGCGCCTGGGCGGTGCTCCCCCGGCCACGGCCGATCCGCAGCAGGTAGACAAGCAGCAGCAGGCAGCTGCCTCCCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCACAAAGt                                                                                                   >  7:122242/1‑149 (MQ=255)
                                        cGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGGCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTcc                                                                                                 <  5:281171/149‑1 (MQ=255)
                                         gAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTc                                                                                                  >  3:183319/1‑147 (MQ=255)
                                                gTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGa                                                                                         >  6:99840/1‑149 (MQ=255)
                                                       ccTGGGCGGTGATCACCAGGCCACGGCCGAGCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTg                                                                                  <  4:85810/149‑1 (MQ=255)
                                                              ggTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCt                                                                                                                 <  3:181196/111‑1 (MQ=255)
                                                              ggTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCt                                                                                                                 >  4:181196/1‑111 (MQ=255)
                                                              ggTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAag                                                                             >  2:179481/1‑147 (MQ=255)
                                                                tGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAagcagc                                                                         <  1:179481/149‑1 (MQ=255)
                                                                  aTCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTcc                                                                                                 <  6:181676/123‑1 (MQ=255)
                                                                  aTCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTcc                                                                                                 >  5:181676/1‑123 (MQ=255)
                                                                     aCCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACCGCGATGATGAAGTCGGTGTGCCTTTCCGGCAAAACGTCCAGGTGCGACTGCGTGCAAAGCagccagcc                                                                    >  8:116094/1‑149 (MQ=255)
                                                                      ccAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGTCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCGAAGCAGCCAGGCt                                                                   >  8:43089/1‑149 (MQ=255)
                                                                       cAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCtt                                                                  <  1:67492/149‑1 (MQ=255)
                                                                       cAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCtt                                                                  >  4:262101/1‑149 (MQ=255)
                                                                        aGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCttt                                                                 <  8:217784/149‑1 (MQ=255)
                                                                        aGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCttt                                                                 <  5:99840/149‑1 (MQ=255)
                                                                              cGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCaaa                                                           <  4:183319/149‑1 (MQ=255)
                                                                                        gcagcagGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              >  6:41108/1‑120 (MQ=255)
                                                                                        gcagcagGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              <  5:41108/120‑1 (MQ=255)
                                                                                             agGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGcc                                            >  3:130098/1‑149 (MQ=255)
                                                                                               gTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGa                                          >  1:212962/1‑149 (MQ=255)
                                                                                                  gACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCttt                                                                 <  8:287081/123‑1 (MQ=255)
                                                                                                     aaTCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATcgccg                                    >  2:81420/1‑149 (MQ=255)
                                                                                                       tCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCgccgcc                                  >  3:188315/1‑149 (MQ=255)
                                                                                                        cagcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              <  4:270815/104‑1 (MQ=255)
                                                                                                        cagcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              >  3:270815/1‑104 (MQ=255)
                                                                                                         agcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCtt                                >  5:255813/1‑149 (MQ=255)
                                                                                                          gcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCtt                                >  3:19758/1‑148 (MQ=255)
                                                                                                          gcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCtt                                <  4:19758/148‑1 (MQ=255)
                                                                                                                 gcagATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAg                                                                                               <  5:94034/78‑1 (MQ=255)
                                                                                                                 gcagATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAg                                                                                               >  6:94034/1‑78 (MQ=255)
                                                                                                                 gcagATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGga                        >  4:114809/1‑149 (MQ=255)
                                                                                                                   agATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGgat                       >  2:240469/1‑148 (MQ=255)
                                                                                                                         cTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTc                >  6:73042/1‑149 (MQ=255)
                                                                                                                           aCCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTcc               >  1:253905/1‑148 (MQ=255)
                                                                                                                             cAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAAc            <  4:130098/149‑1 (MQ=255)
                                                                                                                               ggCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCg          >  7:103901/1‑149 (MQ=255)
                                                                                                                                  cGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTg       <  4:188315/149‑1 (MQ=255)
                                                                                                                                   ttcatcatCGCCGTGCTCGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGc      >  3:254104/8‑149 (MQ=255)
                                                                                                                                     aTTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACAGGTGcc     >  3:33977/1‑148 (MQ=255)
                                                                                                                                       tcatCGCCGTGCTCGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCaa  >  1:212847/4‑149 (MQ=255)
                                                                                                                                          tCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              >  3:267822/1‑70 (MQ=255)
                                                                                                                                          tCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCaa                                                                              <  4:267822/70‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
ACAAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAA  >  NC_002947/5469036‑5469319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑