| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,472,797 | C→T | 7.2% | G8S (GGC→AGC) | rsfS ← | ribosomal silencing factor RsfS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,472,797 | 0 | C | T | 7.2% | 158.3 / 2.4 | 55 | G8S (GGC→AGC) | rsfS | ribosomal silencing factor RsfS |
| Reads supporting (aligned to +/- strand): ref base C (28/23); new base T (2/2); total (30/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
GCGTTGATCTGGCGGTTGGAGGTACCGGTGGCAATGATCATGTAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCG > NC_002947/5472655‑5472943 | gcgTTGATCTGGCGGTTGGAGGTACCGGTGGCAATGATCATGTAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAAt > 1:272370/1‑149 (MQ=255) gTTGATCTGGCGGTTGGAGGTACCGGTGGCAATGATCATGTAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAAttt > 6:143861/1‑149 (MQ=255) tGGCGGTTGGAGGTACCGGTGGCAATGATCATGTAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCtt > 3:82632/1‑149 (MQ=255) gCGGTTGGAGGTACCGGTTGCAATGATCATGTAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGc < 8:248326/145‑1 (MQ=255) gCGGTTGGAGGTACCGGTGGCAATGATCATGTAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTgg > 6:14335/1‑149 (MQ=255) aCCGGTGGCAATGATCATGTAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTNTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCTGTAAATTTTCTGCTTGGTCATATAAAACt > 3:134244/1‑149 (MQ=255) aCCGGTGGCAATGATCATGTAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCTGTAAATTTTCTGCTTGGTCATATAAAACt > 3:129345/1‑149 (MQ=255) ccGGTGGCAATGATCATGTAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTc < 5:14335/149‑1 (MQ=255) gTGGCAATGATCATGTAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGtt > 1:130157/1‑149 (MQ=255) tAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCTGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGAt < 1:242971/148‑1 (MQ=255) tCGGTCAGGCTGTGCTTCTCGCGCACNTCGATGACCNGGATNTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCTGTAAATTTTCTGCTTGGTCATATAANACTCGTTCAACTCGTTGGATGAGgc < 4:134244/149‑1 (MQ=255) tCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCTGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGgc < 4:129345/149‑1 (MQ=255) gTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGgcgc > 5:71127/1‑148 (MQ=255) aGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCTCTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCaga < 4:56843/149‑1 (MQ=255) gCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCagag > 5:179517/1‑148 (MQ=255) gCTGGGCCTCTCGCGCACGTCGATGACCTGTATGTCCTGGGCCTTTACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCagag < 6:179517/148‑1 (MQ=255) cTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGt > 5:68071/1‑149 (MQ=255) ttCTCGCGCACGTCGATGACCTGCATGTCCTGGGCATTGACGTCTTCCAGCGCTGACTTGGTCACTCCGACCTGTACTTC‑GCCGTAAATTTTCTGGTTGGTCATATTAAACTCGTTCAACTCGTTCGATGAGGCGCCAGAGGCACCGt > 2:186393/1‑148 (MQ=255) gcgcACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGtt < 7:6206/148‑1 (MQ=255) cGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCAc > 6:148861/1‑148 (MQ=255) gctGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTc < 6:71127/146‑1 (MQ=255) tGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTg > 8:102351/1‑149 (MQ=255) gTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTc < 3:195554/87‑1 (MQ=255) gTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTc > 4:195554/1‑87 (MQ=255) tGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCt > 3:179501/1‑149 (MQ=255) tGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCt > 3:26329/1‑149 (MQ=255) gggCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTc > 7:276659/1‑149 (MQ=255) ttGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCAc < 3:152319/125‑1 (MQ=255) ttGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCAc > 4:152319/1‑125 (MQ=255) tGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGata > 1:159139/1‑147 (MQ=255) gACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGatat < 3:72497/147‑1 (MQ=255) aCGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAg < 5:143861/148‑1 (MQ=255) aCGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAg < 5:148861/148‑1 (MQ=255) cGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGc > 1:176810/1‑149 (MQ=255) cGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGc < 2:272370/149‑1 (MQ=255) tCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCa < 2:159139/149‑1 (MQ=255) cTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCata > 4:46893/1‑62 (MQ=255) cTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCata < 3:46893/62‑1 (MQ=255) cTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGTGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAg > 2:86264/1‑149 (MQ=255) cAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACg > 2:163319/1‑149 (MQ=255) aGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTg > 1:96809/1‑126 (MQ=255) aGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTg < 2:96809/126‑1 (MQ=255) cTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAATTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGCGTc > 4:99154/1‑149 (MQ=255) ccTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGCGTcc < 4:82632/147‑1 (MQ=255) ggTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAATTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCAc < 3:99154/148‑1 (MQ=255) aCTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAgg > 4:299859/1‑148 (MQ=255) aCTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAgg > 1:21148/1‑148 (MQ=255) aCTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAgg > 8:25502/1‑148 (MQ=255) cTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCAc < 2:159732/97‑1 (MQ=255) cTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCAc < 4:156003/97‑1 (MQ=255) cTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGaa < 2:176810/149‑1 (MQ=255) ccAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGc < 8:127270/128‑1 (MQ=255) aGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGata > 6:33960/1‑93 (MQ=255) aGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCg < 4:26329/149‑1 (MQ=255) cggcGTAAATTTTCTTCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGTGCGCAAGATATAGTTCTGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGAtt < 1:86264/143‑1 (MQ=255) gCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCg > 3:284815/1‑149 (MQ=255) | GCGTTGATCTGGCGGTTGGAGGTACCGGTGGCAATGATCATGTAGTCGGTCAGGCTGTGCTTCTCGCGCACGTCGATGACCTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTC‑GCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTC‑GTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCG > NC_002947/5472655‑5472943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |