| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,485,153 | A→G | 25.0% | intergenic (+59/‑88) | purH → / → purD | bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,485,153 | 0 | A | G | 25.0% | 18.7 / 4.9 | 16 | intergenic (+59/‑88) | purH/purD | bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase |
| Reads supporting (aligned to +/- strand): ref base A (5/7); new base G (2/2); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
TTCGATGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAA‑GCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGG > NC_002947/5485007‑5485291 | ttCGATGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGAtg < 5:188304/149‑1 (MQ=255) gCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGa < 7:301180/148‑1 (MQ=255) cGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGAt < 3:254191/148‑1 (MQ=255) gAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGAtggt < 7:171757/135‑1 (MQ=255) gAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGAtggt > 8:171757/1‑135 (MQ=255) tCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCAccatcgtat > 7:202632/1‑140 (MQ=37) tCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCAccatcgtat < 8:202632/147‑8 (MQ=37) tCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCATGCCCCCGAATACGAAGGGGGATTAACCGCCCCaata > 6:81607/1‑146 (MQ=255) gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg < 8:197991/148‑1 (MQ=255) tgcCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGGCGGTGAATCCAccatcgta < 1:200795/136‑7 (MQ=37) tgcCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGGCGGTGAATCCAccatcgta > 2:200795/1‑130 (MQ=37) cgATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGGTTAACCGCCGCAATAGCGGGCCA‑GCCCGCTCCCACAGTGTTCGgc > 1:207953/1‑149 (MQ=39) gCATGCGCCACTTCCGCCACTAATTACGCGTATCCCGAGGCAAGTCGATATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAACGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGAt < 1:220999/149‑1 (MQ=255) cgcCACTTCCGCCACTAATTCCGCGGATCCGGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAA‑GCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACtt < 5:81607/148‑1 (MQ=255) gcgAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAA‑GCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctgg > 5:73299/1‑148 (MQ=255) gcgAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAA‑GCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATAGGCAGCGGTGGCCGTGAGCACGCcctggcctgg > 5:317114/1‑148 (MQ=255) | TTCGATGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAA‑GCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGG > NC_002947/5485007‑5485291 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |