Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,491,483 C→T 6.3% intergenic (‑19/+33) PP_5715 ← / ← PP_5716 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,491,4830CT 6.3% 180.5 / 2.9 63intergenic (‑19/+33)PP_5715/PP_5716hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/27);  new base T (2/2);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

CCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGC  >  NC_002947/5491337‑5491629
                                                                                                                                                  |                                                                                                                                                  
cccTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGt                                                                                                                                                  >  5:15046/1‑149 (MQ=255)
            gTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTc                                                                                                                                      >  4:236856/1‑149 (MQ=255)
                ggTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGAcg                                                                                                                                  >  7:268778/1‑148 (MQ=255)
                        gCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCATGTCGACAGGCCAACt                                                                                                                          >  3:185077/1‑149 (MQ=255)
                         ccAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGcctgga                                                                                                                          <  8:268778/148‑5 (MQ=255)
                           aGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCa                                                                                                                       >  5:109047/1‑149 (MQ=255)
                                 aCATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCa                                                                                                                 <  2:67797/149‑1 (MQ=255)
                                        aCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTc                                                                                                           >  1:160894/1‑148 (MQ=255)
                                           gTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                       >  3:99541/1‑149 (MQ=255)
                                              cTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGTCTATAGCCGTACACCCACAAAGTGTAATTGGCTTGCATCAGGATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGc                                                                                                     <  3:185383/148‑1 (MQ=255)
                                              cTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGc                                                                                                     <  3:247469/148‑1 (MQ=255)
                                                   gCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGAcgg                                                                                                                                 <  4:232534/115‑3 (MQ=255)
                                                   gCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGAcgg                                                                                                                                 >  3:232534/1‑113 (MQ=255)
                                                    cTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCcagca                                                                                                <  6:109047/147‑1 (MQ=255)
                                                     tCGAGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGa                                                                                             >  8:35383/1‑149 (MQ=255)
                                                       gTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGAcc                                                                                           >  1:128476/1‑149 (MQ=255)
                                                         gAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTg                                                                                         >  8:190028/1‑149 (MQ=255)
                                                         gAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTg                                                                                         >  3:74771/1‑149 (MQ=255)
                                                            gCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAg                                                                                      <  6:290066/149‑1 (MQ=255)
                                                             cACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAgg                                                                                     <  1:24811/149‑1 (MQ=255)
                                                                tGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTT‑CAGTGCCAGCAGGACCTGCAGGGCAt                                                                                 >  1:44986/1‑149 (MQ=255)
                                                                  aCATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCa                                                                                  <  2:126407/147‑1 (MQ=255)
                                                                   cATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTg                                                                               <  5:291674/149‑1 (MQ=255)
                                                                    aTTGTTGTCGATCAATACCAGGCTATTGNCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCACGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCAttct                                                                              >  1:188773/1‑147 (MQ=255)
                                                                    aTTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATACCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgt                                                                              >  7:279079/1‑149 (MQ=255)
                                                                     ttgttgTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtc                                                                             <  6:15046/149‑1 (MQ=255)
                                                                     ttgttgTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCATGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtc                                                                             <  4:185077/149‑1 (MQ=255)
                                                                     tcgctgtCGATCAATACCAGGCTATTCCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtc                                                                             <  8:113514/145‑1 (MQ=255)
                                                                          gtCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCa                                                                                                                       >  4:10281/1‑102 (MQ=255)
                                                                          gtCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCa                                                                                                                       <  3:10281/102‑1 (MQ=255)
                                                                             gATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGtt                                                                     <  4:99541/149‑1 (MQ=255)
                                                                                 aaTACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTgg                                                                  <  7:190028/148‑1 (MQ=255)
                                                                                  aTACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGtt                                                                <  2:160894/149‑1 (MQ=255)
                                                                                      cAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTAATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTa                                                            >  7:205101/1‑149 (MQ=255)
                                                                                         gCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCAtt                                                                                <  6:293566/126‑1 (MQ=255)
                                                                                         gCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCAtt                                                                                >  5:293566/1‑126 (MQ=255)
                                                                                                   ttCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCACCTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAAc                                               >  7:298384/1‑149 (MQ=255)
                                                                                                       aCCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTgg                                                                  >  6:318669/1‑126 (MQ=255)
                                                                                                        ccACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCTGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGt                                          <  1:99879/149‑1 (MQ=255)
                                                                                                        ccACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGt                                          >  6:298825/1‑149 (MQ=255)
                                                                                                         cacaAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGgcgc                                                   >  3:104074/1‑139 (MQ=255)
                                                                                                         cacaAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGgcgc                                                   <  4:104074/139‑1 (MQ=255)
                                                                                                          acaAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                  >  4:249626/1‑59 (MQ=255)
                                                                                                           caAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCg                                        >  6:333022/1‑148 (MQ=255)
                                                                                                                     ttGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCggcggc                              >  3:273639/1‑148 (MQ=255)
                                                                                                                     ttGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGGCGGCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCt                             <  6:71930/149‑1 (MQ=255)
                                                                                                                          ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTg                                                                   >  3:159753/1‑106 (MQ=255)
                                                                                                                          ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTg                                                                   <  4:159753/106‑1 (MQ=255)
                                                                                                                          ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCg                         >  5:329223/1‑148 (MQ=255)
                                                                                                                                 aGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCaa                 <  5:333022/149‑1 (MQ=255)
                                                                                                                                 aGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCaa                 <  4:273639/149‑1 (MQ=255)
                                                                                                                                 aGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTT‑CAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCaa                 <  2:44986/148‑1 (MQ=255)
                                                                                                                                   tATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTAt                                     <  6:314584/127‑1 (MQ=255)
                                                                                                                                   tATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTAt                                     >  5:314584/1‑127 (MQ=255)
                                                                                                                                   tATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAaca               <  8:205101/149‑1 (MQ=255)
                                                                                                                                      gCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCg            >  7:287874/1‑149 (MQ=255)
                                                                                                                                      gCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTAGTCAAGTTTGGTTTCTACAATGGCGCGAACTATGTCGTATCGGCGGCTATCGAAATTAAAAACCg            >  8:138318/1‑149 (MQ=255)
                                                                                                                                       cccTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGc           <  5:298825/149‑1 (MQ=255)
                                                                                                                                        ccTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGaa                       <  4:280077/136‑1 (MQ=255)
                                                                                                                                        ccTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGaa                       >  3:280077/1‑136 (MQ=255)
                                                                                                                                        ccTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGca          >  7:183456/1‑149 (MQ=255)
                                                                                                                                        ccTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGca          >  1:190227/1‑149 (MQ=255)
                                                                                                                                          tCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCAACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGcac         >  1:270028/1‑148 (MQ=255)
                                                                                                                                                 ccGTCGACGCCGTGTCGACAGGCCAACGTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGGCAAGTTTGGTTTCTACAATGGCGGGAACGTTGTGGTATCGGCGGCTATCGAAATTCACCACCGCACCACAGGc  >  7:284710/1‑148 (MQ=255)
                                                                                                                                                  cGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTc                   <  2:155958/130‑1 (MQ=255)
                                                                                                                                                  cGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTc                   >  1:155958/1‑130 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGC  >  NC_002947/5491337‑5491629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑