| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 103,819 | T→A | 7.2% | intergenic (+117/‑23) | PP_0098 → / → PP_0099 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 103,819 | 0 | T | A | 7.2% | 160.3 / 3.2 | 56 | intergenic (+117/‑23) | PP_0098/PP_0099 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (25/27); new base A (2/2); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
TTGTTTGGTGCAGTGCTGCAGCCCTAAGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGAAGTGCCGCAAACCCAGGGTACC > NC_002947/103676‑103967 | ttgttTGGTGCAGTGCTGCAGCCCTAAGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATa < 4:255041/149‑1 (MQ=255) tGCAGTGCTGCAGCCCTAAGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATTAATATACAGTAtcg < 8:295201/149‑3 (MQ=255) gCAGTGCTGGTGCCCTAAGCGCTGTCGGTCTGGGATCGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATTAATATACAGTAtcgt < 7:203288/149‑4 (MQ=255) aGTGCTGCAGCCCTAAGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGAGTATGTGCGCTGGCATGGCCTGAAGTAGTCATACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAgg < 5:291992/149‑1 (MQ=255) gTGCTGCAGCCCTAAGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGt < 3:116018/149‑1 (MQ=255) cAGCCCTAAGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGcc < 8:61909/148‑1 (MQ=255) gCCCTAAGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCAt > 4:258427/1‑148 (MQ=255) ccTAAGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGAt < 7:54521/149‑1 (MQ=255) cTAAGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGAtt > 2:177400/1‑149 (MQ=255) tAAGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATtc > 3:158760/1‑149 (MQ=255) aGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATtcct > 3:136930/1‑149 (MQ=255) aGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATtcct < 3:258427/149‑1 (MQ=255) aGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATtc < 8:113252/147‑1 (MQ=255) tctgtctgGGATGGGCGGCAATCGAGCAAGGCAGAGTATGTGCGCTGGCATGGCCTGAAGTATTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTAAATTAATACAGTATTCGGGTGAGCAATGATTCCTCCAGTAc > 2:181458/1‑149 (MQ=255) tgGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCaa > 4:301036/1‑148 (MQ=255) gggATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCaa > 2:58484/1‑147 (MQ=255) ggATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTAcc < 4:134617/142‑1 (MQ=255) ggATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTAcc > 3:134617/1‑142 (MQ=255) ggATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAg < 7:166681/149‑1 (MQ=255) gATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAgg < 1:69613/149‑1 (MQ=255) gATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAgg > 2:69613/1‑149 (MQ=255) aTGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAggg > 1:161653/1‑149 (MQ=255) gcggcAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGt < 1:58484/149‑1 (MQ=255) ggcAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTgg > 1:223154/1‑149 (MQ=255) cAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCa > 3:82198/1‑149 (MQ=255) gAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGgcg > 1:17908/1‑149 (MQ=255) aGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGgcg > 4:98835/1‑148 (MQ=255) aGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGca > 7:73823/1‑146 (MQ=255) aGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGca < 8:73823/146‑1 (MQ=255) cAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGt < 3:47854/132‑1 (MQ=255) cAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGt > 4:47854/1‑132 (MQ=255) cGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCcaca < 4:136930/149‑1 (MQ=255) cGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCcaca < 3:98835/149‑1 (MQ=255) tgCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAAcc < 3:301036/148‑1 (MQ=255) cgcTGGCATGGCCTGAAGTTGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCt > 4:319461/1‑149 (MQ=255) cTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGGTGGTAAGATCCCGGTGTGCACGATACTGTATATTATTACAGTATTCGGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCttt < 1:181458/149‑1 (MQ=255) ggCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGc > 1:100162/1‑149 (MQ=255) gCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGcc > 2:95304/1‑149 (MQ=255) gCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGc < 1:177400/148‑1 (MQ=255) gCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTgg < 4:158760/149‑1 (MQ=255) aGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGTCCGTGGCACGGCGCACAATCAACACAACCGCATTGCCCCTGGTAATTCGGTGGCg > 2:28009/1‑149 (MQ=255) aGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGc < 2:17908/148‑1 (MQ=255) ccAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGaa < 2:161653/149‑1 (MQ=255) cAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAg < 3:319461/149‑1 (MQ=255) cTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATgg < 4:82198/148‑1 (MQ=255) aaGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTa > 5:96594/1‑149 (MQ=255) cccGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAg > 6:136455/1‑148 (MQ=255) cGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGATCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGaa < 2:100162/149‑1 (MQ=255) ggTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGAAg > 6:222924/1‑149 (MQ=255) ctgaaTACTGTATTAATATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGAAGTGCCg > 5:60722/5‑148 (MQ=255) cACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGACGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGAAGTGCCg > 5:157784/1‑148 (MQ=255) tgaaTACTGTATTAATATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGAAGTGCCGc > 3:319048/4‑148 (MQ=255) cTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCTCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGTTGGCTGGTACCAGGAAGTGCCGCAAACCCa < 1:28009/149‑1 (MQ=255) tGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGAAGTGCCGCAAACCCAg > 1:85960/1‑149 (MQ=255) tataTTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGAAGTGCCGCAAACCCAgg < 6:96594/148‑1 (MQ=255) tataTTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGAAGTGCCGCAAACCCAgg < 5:222924/148‑1 (MQ=255) atTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGAAGTGCCGCAAACCCAGGGTAc > 5:240303/1‑149 (MQ=255) ttAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGAAGTGCCGCAAACCCAGGGTAcc > 5:232667/1‑149 (MQ=255) | TTGTTTGGTGCAGTGCTGCAGCCCTAAGCGCTGTCTGTCTGGGATGGGCGGCAATCGAGCCAGGCAGCGTATGTGCGCTGGCATGGCCTGAAGTAGTCAGACCAGAGGTGCTGGTAAGATCCCGGTGTGCACGATACTGTATATTAATACAGTATTCAGGTGAGCCATGATTCCTCCAGTACCGCAAAAGGGCCGTGGCACGGCGCACAATCCACACAACCGCTTTGCCCCTGGTCATTCGGTGGCGGAAGATGATGGCTGGTACCAGGAAGTGCCGCAAACCCAGGGTACC > NC_002947/103676‑103967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |