| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,570,178 | G→C | 12.7% | A24G (GCA→GGA) ‡ | nnrD ← | ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,570,178 | 0 | G | C | 12.7% | 149.0 / 11.7 | 55 | A24G (GCA→GGA) ‡ | nnrD | ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (22/26); new base C (3/4); total (25/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01 | |||||||||||
GTGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCC > NC_002947/5570030‑5570322 | gTGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCtgccgtg > 8:45326/1‑149 (MQ=255)gTGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCtgccgtg > 2:154151/1‑149 (MQ=255) cTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCtgccgtgcc < 1:56569/148‑1 (MQ=255) cccggTTTCCACCCTGCCCAGCCCGGCGCCTCCACCCACTGCGGCTTCGTGACTTTTCAACACAGCGCCTCCGGTTCCCCTGTCGCCCCCGACCACCAGTACTTGGCCAAATTCGCCTTTTTGG‑GTCTGCGCCTGCCGGCACGGCAGGc < 7:204410/146‑1 (MQ=255) ccGGGGTGGCACGGTGTCCAGCCCGGCGGCGGACCGCAGGGCGGCTTCGGCACTGCGCAAACCAGCGCCGCCGGTTCCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GACTGCGCCTGCCGGCACGGCAGGc < 7:205821/149‑1 (MQ=255) ttGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAACGTCGCCTTTGTGG‑GCATGCGCCTGCCGGCACGGCAGGCgcatg < 8:65205/149‑5 (MQ=255) cACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACCGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCaa > 6:189216/1‑146 (MQ=255) cACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCaa < 5:189216/146‑1 (MQ=255) ccgcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGGCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGCGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAAtt < 1:82358/149‑1 (MQ=255) aaCGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg > 2:327018/1‑149 (MQ=255) gcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg < 7:45326/149‑1 (MQ=255) ttCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGgt > 6:58373/1‑149 (MQ=255) cGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGgt < 3:37497/147‑1 (MQ=255) cGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGgt < 7:85478/147‑1 (MQ=255) aCTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgg > 2:221999/1‑140 (MQ=255) aCTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgg < 1:221999/140‑1 (MQ=255) gAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg < 4:121952/145‑1 (MQ=255) gAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg > 3:121952/1‑145 (MQ=255) aGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTc < 5:58373/147‑1 (MQ=255) cAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCg < 1:277280/149‑1 (MQ=255) gcgcCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg > 7:152021/1‑149 (MQ=255) gcgcCTCCGGGACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg < 4:20648/149‑1 (MQ=255) gcCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGt > 2:215122/1‑149 (MQ=255) tACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTTGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCt > 2:235002/1‑149 (MQ=255) ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTcc > 1:323148/1‑148 (MQ=255) aGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg < 3:220027/102‑1 (MQ=255) aGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg > 4:220027/1‑102 (MQ=255) cccccgGACCAGCATGTCATGGCGAAAGCCGCCTTTGTGG‑TCAAGCGCCTGCCTTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCTTTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCt < 6:324377/147‑1 (MQ=255) ccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg > 5:237838/1‑148 (MQ=255) ccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg > 6:255412/1‑148 (MQ=255) cccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAgg < 2:302150/134‑1 (MQ=255) ccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg > 1:320664/1‑146 (MQ=255) ccaccaGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAAtt < 8:257162/149‑1 (MQ=255) caccaGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATta > 4:162973/1‑149 (MQ=255) ccaGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtata < 2:320664/149‑1 (MQ=255) caGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATAc < 1:154151/149‑1 (MQ=255) cATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCAc < 1:215122/148‑1 (MQ=255) aTGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTc < 4:199220/122‑1 (MQ=255) aTGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTc > 3:199220/1‑122 (MQ=255) cAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGttt < 8:77913/91‑1 (MQ=255) cAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGttt > 7:77913/1‑91 (MQ=255) cAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGcc > 6:92696/1‑149 (MQ=255) tCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAt > 8:308787/1‑149 (MQ=255) gCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGc > 1:294950/1‑91 (MQ=255) gCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGc > 1:298738/1‑91 (MQ=255) gCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGc < 2:294950/91‑1 (MQ=255) gCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGc < 2:298738/91‑1 (MQ=255) cTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgcc < 7:308787/149‑1 (MQ=255) gtgG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc > 3:54325/1‑149 (MQ=255) tgG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc < 2:323148/148‑1 (MQ=255) gCATGCGCCTGACGTGCCGGCAGGCGGTCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccat < 8:152021/149‑1 (MQ=255) aTGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatcc < 6:237838/149‑1 (MQ=255) ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc < 5:23466/140‑1 (MQ=255) ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc > 6:23466/2‑141 (MQ=255) ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatccat > 7:140184/2‑149 (MQ=255) ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatccat > 5:109228/2‑149 (MQ=255) cgcCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatccat < 4:248925/148‑1 (MQ=255) ccTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTc > 8:160118/1‑149 (MQ=255) ccgtgccgGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGcc > 5:146091/1‑149 (MQ=255) | GTGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCC > NC_002947/5570030‑5570322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |