Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,570,179 C→A 12.6% A24S (GCA→TCA) ‡ nnrD ← ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,570,1790CA12.6% 145.4 / 9.8 55A24S (GCA→TCA) ‡nnrDADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (22/26);  new base A (3/4);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCC  >  NC_002947/5570033‑5570322
                                                                                                                                                   |                                                                                                                                               
cTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCtgccgtgcc                                                                                                                                                <  1:56569/148‑1 (MQ=255)
      cccggTTTCCACCCTGCCCAGCCCGGCGCCTCCACCCACTGCGGCTTCGTGACTTTTCAACACAGCGCCTCCGGTTCCCCTGTCGCCCCCGACCACCAGTACTTGGCCAAATTCGCCTTTTTGG‑GTCTGCGCCTGCCGGCACGGCAGGc                                                                                                                                         <  7:204410/146‑1 (MQ=255)
      ccGGGGTGGCACGGTGTCCAGCCCGGCGGCGGACCGCAGGGCGGCTTCGGCACTGCGCAAACCAGCGCCGCCGGTTCCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GACTGCGCCTGCCGGCACGGCAGGc                                                                                                                                         <  7:205821/149‑1 (MQ=255)
           ttGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAACGTCGCCTTTGTGG‑GCATGCGCCTGCCGGCACGGCAGGCgcatg                                                                                                                                    <  8:65205/149‑5 (MQ=255)
               cACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACCGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCaa                                                                                                                                   >  6:189216/1‑146 (MQ=255)
               cACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCaa                                                                                                                                   <  5:189216/146‑1 (MQ=255)
                                  ccgcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGGCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGCGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAAtt                                                                                                              <  1:82358/149‑1 (MQ=255)
                                      aaCGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                         >  2:327018/1‑149 (MQ=255)
                                                 gcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                              <  7:45326/149‑1 (MQ=255)
                                                   ttCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGgt                                                                                            >  6:58373/1‑149 (MQ=255)
                                                     cGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGgt                                                                                            <  3:37497/147‑1 (MQ=255)
                                                     cGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGgt                                                                                            <  7:85478/147‑1 (MQ=255)
                                                         aCTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgg                                                                                               <  1:221999/140‑1 (MQ=255)
                                                         aCTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgg                                                                                               >  2:221999/1‑140 (MQ=255)
                                                            gAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg                                                                                       <  4:121952/145‑1 (MQ=255)
                                                            gAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg                                                                                       >  3:121952/1‑145 (MQ=255)
                                                             aGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTc                                                                                    <  5:58373/147‑1 (MQ=255)
                                                               cAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCg                                                                                <  1:277280/149‑1 (MQ=255)
                                                                      gcgcCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg                                                                         >  7:152021/1‑149 (MQ=255)
                                                                      gcgcCTCCGGGACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg                                                                         <  4:20648/149‑1 (MQ=255)
                                                                        gcCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGt                                                                       >  2:215122/1‑149 (MQ=255)
                                                                                tACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTTGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCt                                                               >  2:235002/1‑149 (MQ=255)
                                                                                   ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTcc                                                             >  1:323148/1‑148 (MQ=255)
                                                                                     aGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                         <  3:220027/102‑1 (MQ=255)
                                                                                     aGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                         >  4:220027/1‑102 (MQ=255)
                                                                                          cccccgGACCAGCATGTCATGGCGAAAGCCGCCTTTGTGG‑TCAAGCGCCTGCCTTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCTTTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCt                                                      <  6:324377/147‑1 (MQ=255)
                                                                                            ccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                    >  6:255412/1‑148 (MQ=255)
                                                                                            ccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                    >  5:237838/1‑148 (MQ=255)
                                                                                             cccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAgg                                                                 <  2:302150/134‑1 (MQ=255)
                                                                                              ccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                    >  1:320664/1‑146 (MQ=255)
                                                                                                  ccaccaGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAAtt                                             <  8:257162/149‑1 (MQ=255)
                                                                                                   caccaGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATta                                            >  4:162973/1‑149 (MQ=255)
                                                                                                     ccaGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtata                                          <  2:320664/149‑1 (MQ=255)
                                                                                                      caGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATAc                                         <  1:154151/149‑1 (MQ=255)
                                                                                                           cATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCAc                                     <  1:215122/148‑1 (MQ=255)
                                                                                                            aTGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTc                                                              <  4:199220/122‑1 (MQ=255)
                                                                                                            aTGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTc                                                              >  3:199220/1‑122 (MQ=255)
                                                                                                                 cAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGttt                                                                                        <  8:77913/91‑1 (MQ=255)
                                                                                                                 cAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGttt                                                                                        >  7:77913/1‑91 (MQ=255)
                                                                                                                 cAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGcc                              >  6:92696/1‑149 (MQ=255)
                                                                                                                      tCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAt                         >  8:308787/1‑149 (MQ=255)
                                                                                                                        gCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGc                                                                                 >  1:294950/1‑91 (MQ=255)
                                                                                                                        gCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGc                                                                                 >  1:298738/1‑91 (MQ=255)
                                                                                                                        gCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGc                                                                                 <  2:294950/91‑1 (MQ=255)
                                                                                                                        gCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGc                                                                                 <  2:298738/91‑1 (MQ=255)
                                                                                                                          cTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgcc                     <  7:308787/149‑1 (MQ=255)
                                                                                                                              gtgG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc                 >  3:54325/1‑149 (MQ=255)
                                                                                                                               tgG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc                 <  2:323148/148‑1 (MQ=255)
                                                                                                                                   gCATGCGCCTGACGTGCCGGCAGGCGGTCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccat             <  8:152021/149‑1 (MQ=255)
                                                                                                                                     aTGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatcc           <  6:237838/149‑1 (MQ=255)
                                                                                                                                       ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc                 <  5:23466/140‑1 (MQ=255)
                                                                                                                                       ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc                 >  6:23466/2‑141 (MQ=255)
                                                                                                                                       ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatccat         >  7:140184/2‑149 (MQ=255)
                                                                                                                                       ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatccat         >  5:109228/2‑149 (MQ=255)
                                                                                                                                        cgcCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatccat         <  4:248925/148‑1 (MQ=255)
                                                                                                                                          ccTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTc      >  8:160118/1‑149 (MQ=255)
                                                                                                                                              ccgtgccgGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGcc  >  5:146091/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGG‑GCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCC  >  NC_002947/5570033‑5570322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑