Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,573,867 A→T 8.4% A317A (GCA→GCT)  rsgA → ribosome biogenesis GTPase RsgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,573,8670AT 8.4% 163.3 / 3.6 56A317A (GCA→GCT) rsgAribosome biogenesis GTPase RsgA
Reads supporting (aligned to +/- strand):  ref base A (25/26);  new base T (2/3);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTGTAGGAGCGGGTTTACCCGCGAATACGGTG  >  NC_002947/5573729‑5574010
                                                                                                                                          |                                                                                                                                                
tATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAgg                                                                                                                                        <  3:294836/149‑1 (MQ=255)
 aTTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAggg                                                                                                                                       >  2:250498/1‑149 (MQ=255)
     gcgAGTTCGGCTTTGGCCATGTCAGCCGCGAGGACGTGGAGGATGGCTTCTTCGAGTTCCGCGACGTGTTCGTGACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCTAGGCTCTGGAAGAGGGgcgc                                                                                                                                   <  2:53918/149‑1 (MQ=255)
      cgAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGATGGGCGCa                                                                                                                                  <  2:105740/149‑1 (MQ=255)
         gTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATc                                                                                                                                >  4:44058/1‑148 (MQ=255)
             ggCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGcc                                                                                                                           >  7:170560/1‑149 (MQ=255)
              gCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCg                                                                                                                          <  8:170560/149‑1 (MQ=255)
                cTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCa                                                                                                                        <  5:90633/149‑1 (MQ=255)
                    gCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAgcg                                                                                                                     >  2:201926/1‑148 (MQ=255)
                            aGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACa                                                                                                             <  2:81284/148‑1 (MQ=255)
                                gcgACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTAc                                                                                                        >  6:72285/1‑149 (MQ=255)
                                       cgTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGa                                                                                                 >  3:231887/1‑149 (MQ=255)
                                        gTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAg                                                                                                                       <  6:294056/126‑1 (MQ=255)
                                        gTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAg                                                                                                                       >  5:294056/1‑126 (MQ=255)
                                         tGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGatc                                                                                               <  2:46253/149‑1 (MQ=255)
                                                      cATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCt                                                                                                          <  4:198063/125‑1 (MQ=255)
                                                      cATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCt                                                                                                          >  3:198063/1‑125 (MQ=255)
                                                           aGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCag                                                                               >  7:45028/1‑147 (MQ=255)
                                                                cgcgACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCTCTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAg                                                                        >  6:39555/1‑149 (MQ=255)
                                                                cgcgACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCTCTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAg                                                                        >  6:34612/1‑149 (MQ=255)
                                                                cgcgACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGa                                                                                                 >  6:311429/1‑124 (MQ=255)
                                                                cgcgACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGa                                                                                                 <  5:311429/124‑1 (MQ=255)
                                                                cgcgACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAg                                                                        >  3:186652/1‑149 (MQ=255)
                                                                  cgACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGc                                                                       >  2:298426/1‑148 (MQ=255)
                                                                     ccTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACt                                                                   >  1:157363/1‑149 (MQ=255)
                                                                      cTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCTCTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTg                                                                  <  5:39555/149‑1 (MQ=255)
                                                                      cTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCTCTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTg                                                                  <  5:34612/149‑1 (MQ=255)
                                                                          tCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAAc                                                              >  6:324351/1‑149 (MQ=255)
                                                                            ggCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGc                                                            >  4:203095/1‑149 (MQ=255)
                                                                              cACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTc                                                          <  1:250498/149‑1 (MQ=255)
                                                                                 cTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCa                                                                                        >  2:13379/1‑116 (MQ=255)
                                                                                 cTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCa                                                                                        <  1:13379/116‑1 (MQ=255)
                                                                                 cTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGa                                                        >  1:151302/1‑148 (MQ=255)
                                                                                  tGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGc                                                                                                     <  7:306977/102‑1 (MQ=255)
                                                                                  tGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGc                                                                                                     >  8:306977/1‑102 (MQ=255)
                                                                                    ccGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGa‑aa                                                     <  3:44058/147‑1 (MQ=255)
                                                                                    ccGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AAt                                                    <  8:222092/148‑1 (MQ=255)
                                                                                      gCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGaa                                                 <  3:203095/149‑1 (MQ=255)
                                                                                       cTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGaaa                                                <  5:324351/149‑1 (MQ=255)
                                                                                       cTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGa                                                  >  3:181057/1‑147 (MQ=255)
                                                                                       cTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGAAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGa                                                  >  6:269948/1‑147 (MQ=255)
                                                                                          ccGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAg                                               <  1:298426/147‑1 (MQ=255)
                                                                                                   cAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATc                                    >  3:280749/1‑149 (MQ=255)
                                                                                                      gCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAggg                                                                                                                                       <  2:185335/48‑1 (MQ=255)
                                                                                                      gCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAggg                                                                                                                                       >  1:185335/1‑48 (MQ=255)
                                                                                                       cATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCtt                                <  4:231887/149‑1 (MQ=255)
                                                                                                        aTGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTg                               <  1:201926/149‑1 (MQ=255)
                                                                                                          gACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTg                               >  2:234765/1‑147 (MQ=255)
                                                                                                           aCCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTGTa                             <  8:45028/148‑1 (MQ=255)
                                                                                                             cgcgAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTGTAGGa                          <  4:186652/149‑1 (MQ=255)
                                                                                                                 aaCCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTGTAGGAGCgg                      <  4:181057/149‑1 (MQ=255)
                                                                                                                  aCCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTGTAGGAGCgg                      <  2:235470/148‑1 (MQ=255)
                                                                                                                  aCCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTGTAGGAGCgg                      >  1:235470/1‑148 (MQ=255)
                                                                                                                     gggCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTGTAGGAGCGGGttt                  <  5:72285/149‑1 (MQ=255)
                                                                                                                              gctgctCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGATCATGAAAGTTGCCGCGATCCCTTGTAGGAGCGGGTTTACCCGCGa          <  1:234765/149‑1 (MQ=255)
                                                                                                                                     aaGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTGTAGGAGCGGGTTTACCCGCGAATACGGTg  >  5:130046/1‑149 (MQ=255)
                                                                                                                                          aCTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTGTAGGAGCGGGTTTACCCGCGaa         >  6:264929/1‑137 (MQ=255)
                                                                                                                                          aCTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTGTAGGAGCGGGTTTACCCGCGaa         <  5:264929/137‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                
TATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAGCGCATGAACAGCTACCGCTCGATCATCGCCAGCCTGGCAGAAGACAGCTACTGAAACGCTCGA‑AATGAAAGTTGCCGCGATCCCTTGTAGGAGCGGGTTTACCCGCGAATACGGTG  >  NC_002947/5573729‑5574010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑