| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 556,997 | A→G | 10.8% | T16A (ACG→GCG) | rpsN → | 30S ribosomal protein S14 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 556,997 | 0 | A | G | 10.8% | 115.5 / 4.9 | 46 | T16A (ACG→GCG) | rpsN | 30S ribosomal protein S14 |
| Reads supporting (aligned to +/- strand): ref base A (21/20); new base G (3/2); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
GACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGC > NC_002947/556855‑557132 | gACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGACGCTTCAGCTCG‑Cg‑g > 2:318079/1‑146 (MQ=255)gACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGACGCTTCAGCTCG‑Cg‑g < 1:318079/146‑1 (MQ=255) accaccACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAGAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGc < 2:37275/147‑1 (MQ=255) ccacTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTaagaa < 1:159944/149‑1 (MQ=255) cacTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTaagaag > 8:51698/1‑149 (MQ=255) cacTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGACCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTaag > 5:51657/1‑146 (MQ=255) cTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGc > 3:243052/1‑137 (MQ=255) cTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGc < 4:243052/137‑1 (MQ=255) gTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CGTGTAGCAAAGTTCGCTAAGAAGCGTgc < 1:171654/147‑1 (MQ=255) ttCGGACGACGAAGGGCGCGCTCTGCTGCTTTCTTTCAAGTTCCCGTTCCGAAAGTTATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTgctga < 7:114600/148‑1 (MQ=255) ggACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTgctgagct > 8:69855/1‑148 (MQ=255) ggACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTACCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTCG‑CG‑Gtttttca > 5:183073/1‑118 (MQ=38) ggACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTACCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTCG‑CG‑Gtttttca < 6:183073/124‑7 (MQ=38) cgacgaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGaaa < 1:36336/149‑1 (MQ=255) gaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACAGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGa > 1:50430/1‑149 (MQ=255) aGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGAc > 2:110523/1‑148 (MQ=255) tgctgcGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAAc < 8:110697/147‑1 (MQ=255) ggcGTGCTTTCAAGTTCCCCTTCCCCAACTGATTGCAGTAGGAAAATGGCCAAGACGACCAGGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGaa < 8:103253/148‑1 (MQ=255) gTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGc > 7:226090/1‑149 (MQ=255) tttCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCaaa > 3:29062/1‑94 (MQ=255) tttCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCaaa < 4:29062/94‑1 (MQ=255) aaGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCtctc > 1:138026/1‑146 (MQ=255) gTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCagaa < 7:51698/149‑1 (MQ=255) tCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCagaa > 8:175107/1‑147 (MQ=255) tCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCagaa > 1:107894/1‑147 (MQ=255) ttCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGtt > 6:103854/1‑149 (MQ=255) gCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGc > 1:317059/1‑149 (MQ=255) ttGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTg < 7:69855/149‑1 (MQ=255) gAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCg < 5:279851/149‑1 (MQ=255) tAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGctc > 1:148401/1‑149 (MQ=255) aaaaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCa < 5:103854/149‑1 (MQ=255) aaaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCa < 2:148401/148‑1 (MQ=255) gCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAag > 7:243785/1‑147 (MQ=255) cAATAAGAGCATGAAAAACCGCTAGCTGAAGCGTCAGCTCACCA‑CCAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagcag < 1:110523/149‑1 (MQ=255) aagaagAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGcc < 2:50430/149‑1 (MQ=255) gaagaGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCAc < 2:317059/149‑1 (MQ=255) gaagaGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCAc > 2:263779/1‑149 (MQ=255) gAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGc < 2:56323/128‑1 (MQ=255) gAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGc > 1:56323/1‑128 (MQ=255) gAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGc > 4:124932/1‑147 (MQ=255) gAAAAACCGCGAGCTGAAGCGACAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCAGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGACCGTTTCGCTGCCGTTGTCGCTCTTCAGAAGCAGCCACGCGACGcaa > 7:73509/1‑147 (MQ=255) aaaaaCCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTa < 2:107894/148‑1 (MQ=255) aaaaCCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAgc < 2:138026/149‑1 (MQ=255) aaaCCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAgcg > 1:210143/1‑149 (MQ=255) cgtgAGCTGACGCTTCAGCTCG‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTc > 1:282142/4‑149 (MQ=255) gAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTgc > 1:195314/1‑149 (MQ=255) | GACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCA‑CG‑GTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGC > NC_002947/556855‑557132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |