Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,619,683 T→C 100% H273R (CAC→CGC)  PP_4938 ← glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,619,6830TC96.8% 194.9 / ‑2.7 61H273R (CAC→CGC) PP_4938glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (29/30);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCTCGCGAAACACTTCCAGGTCGCTGCACACCACCGGTACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGTGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGCGAATGCCTCGATCAGCATTTC  >  NC_002947/5619539‑5619828
                                                                                                                                                |                                                                                                                                                 
tGCTCGCGAAACACTTCCAGGTCGCTGCACACAACCGGTACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGAcc                                                                                                                                               >  8:86894/1‑149 (MQ=255)
       gAAACACTTCCAGGTCGCTGCACACCACCGGTACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGa                                                                                                                                        <  5:183619/149‑1 (MQ=255)
          acacTTCCAGGTCGCTGCACACCACCGGTACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGTGACCGCGCAGATg                                                                                                                                      >  4:30272/1‑148 (MQ=255)
                   ggTCGCTGCACACCACCGGTACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCg                                                                                                                            >  5:185344/1‑149 (MQ=255)
                     tCGCTGCACACCACCGGTACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGc                                                                                                                          <  4:121676/149‑1 (MQ=255)
                        cTGCACACCACCGGTACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGAcc                                                                                                                                               <  8:111741/125‑1 (MQ=255)
                        cTGCACACCACCGGTACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGAcc                                                                                                                                               >  7:111741/1‑125 (MQ=255)
                        cTGCACACCACCGGTACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAg                                                                                                                       <  7:156690/149‑1 (MQ=255)
                           cacaCCACCGGTACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCTACCGCGCAGATGAACCCGCTCGGCCAGGc                                                                                                                     >  2:97575/1‑148 (MQ=255)
                                      tACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGa                                                                                                         <  7:124588/149‑1 (MQ=255)
                                        cATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGTGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATa                                                                                                       <  3:30272/149‑1 (MQ=255)
                                             gCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGTCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACgctg                                                                                                  <  5:60725/149‑1 (MQ=255)
                                             gCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACgctg                                                                                                  >  7:95935/1‑149 (MQ=255)
                                                   aCCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTgcat                                                                                            <  7:86894/149‑2 (MQ=255)
                                                   aCCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTgcag                                                                                            >  4:136507/1‑149 (MQ=255)
                                                    ccGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTgcag                                                                                            >  4:62342/1‑148 (MQ=255)
                                                    ccGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTgcag                                                                                            >  3:265542/1‑148 (MQ=255)
                                                      gCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCAc                                                                                         <  6:90331/149‑1 (MQ=255)
                                                         tctTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGccc                                                                                                                   <  7:79492/120‑1 (MQ=255)
                                                         tctTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGccc                                                                                                                   >  8:79492/1‑120 (MQ=255)
                                                         tctTGCACCACCAGCCCCAGGCCATCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATCAACCCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACgg                                                                                       >  8:183439/1‑148 (MQ=255)
                                                             gCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCAtg                                                                                   <  4:73609/148‑1 (MQ=255)
                                                               accaccAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCCGCAGCATGCCGtgctg                                                                                >  4:282673/1‑149 (MQ=255)
                                                                ccaccaGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGc                                                                                                                     >  6:179003/1‑111 (MQ=255)
                                                                ccaccaGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGc                                                                                                                     <  5:179003/111‑1 (MQ=255)
                                                                ccaccaGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCAtgctgc                                                                               <  8:95935/149‑1 (MQ=255)
                                                                          cAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGc                                                                     >  5:323853/1‑149 (MQ=255)
                                                                           aGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGc                                                                     >  4:125896/1‑148 (MQ=255)
                                                                                ttCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGa                                                               >  8:96852/1‑149 (MQ=255)
                                                                                   ggAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATc                                                             <  4:265542/148‑1 (MQ=255)
                                                                                   ggAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCg                                                            >  4:236742/1‑149 (MQ=255)
                                                                                   ggAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCg                                                            >  2:151621/1‑149 (MQ=255)
                                                                                    gAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGc                                                           <  7:96852/149‑1 (MQ=255)
                                                                                    gAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGc                                                           >  5:225377/1‑149 (MQ=255)
                                                                                          gAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTg                                                     <  3:62342/149‑1 (MQ=255)
                                                                                           aaGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGc                                                    <  6:185344/149‑1 (MQ=255)
                                                                                                   aaTAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCgg                                            <  3:203800/149‑1 (MQ=255)
                                                                                                   aaTAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCgg                                            >  4:203800/1‑149 (MQ=255)
                                                                                                        ccAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGcc                                       >  1:65151/1‑149 (MQ=255)
                                                                                                            tCGAATGCTAGATACAGCTGCTGCAGGTCTTCGCTGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTg                                    >  6:302145/1‑148 (MQ=255)
                                                                                                              gAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCa                                  <  3:236742/148‑1 (MQ=255)
                                                                                                               aaTGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACt                                <  5:32119/149‑1 (MQ=255)
                                                                                                                 tGCTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTgg                              <  3:125896/149‑1 (MQ=255)
                                                                                                                   cTCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGcc                            <  8:158005/149‑1 (MQ=255)
                                                                                                                    tCGATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGccc                           >  7:173435/1‑149 (MQ=255)
                                                                                                                      gATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGa                                                                                                         <  4:193669/69‑1 (MQ=255)
                                                                                                                      gATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGa                                                                                                         >  3:193669/1‑69 (MQ=255)
                                                                                                                      gATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTg                         >  8:64564/1‑149 (MQ=255)
                                                                                                                      gATACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTg                         <  2:65151/149‑1 (MQ=255)
                                                                                                                        tACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGc                       >  3:7819/1‑149 (MQ=255)
                                                                                                                        tACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGc                       <  5:217447/149‑1 (MQ=255)
                                                                                                                        tACAGCTGCTGCAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGc                       <  5:222216/149‑1 (MQ=255)
                                                                                                                                   cAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGtt                                          <  2:114847/119‑1 (MQ=255)
                                                                                                                                   cAGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGCGAATGCCTCGa            <  1:151621/149‑1 (MQ=255)
                                                                                                                                    aGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGCTAATGCCTCGAt           <  4:209246/149‑1 (MQ=255)
                                                                                                                                    aGGTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGCGAATGCCTCGAt           <  8:173435/149‑1 (MQ=255)
                                                                                                                                      gTCTTCGCGGCGACCGCTAAGATGAACTCCCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGAACGGCATGCTGCGGCCCCTCGCCAATGATCGACAGCTGCAAGCACTGTTGCCGTGCACTGGCCCTGGCGAATGccaccataa         >  4:53150/1‑142 (MQ=255)
                                                                                                                                      gTCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGCGAATGCCTCGAt           >  1:199454/1‑147 (MQ=255)
                                                                                                                                       tCTTCGCGGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCTACGCGTCGATACTCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGCGAATGCCTCGATCAg        <  6:225377/149‑1 (MQ=255)
                                                                                                                                           cgcgGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGCGAATGCCTCGATCAGCAtt    >  6:18583/1‑149 (MQ=255)
                                                                                                                                            gcggcgACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGCGAATGCCTCGATCAGCAttt   <  3:136507/149‑1 (MQ=255)
                                                                                                                                            gcggcgACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGCGAATGCCTCGATCAGCAttt   >  5:78674/1‑149 (MQ=255)
                                                                                                                                             cgGCGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGCGAATGCCTCGATCAGCATTTc  >  3:21667/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TGCTCGCGAAACACTTCCAGGTCGCTGCACACCACCGGTACATCGGCCATCACCGCCTCTTGCACCACCAGCCCCAGGCCTTCGGAACGCGAAGGTACCAATAGCCAGTCGAATGCTCGATACAGCTGCTGCAGGTCTTCGCGGTGACCGCGCAGATGAACGCGCTCGGCCAGGCCCAACGCGTCGATACGCTGCTGCAGCACGGCATGCTGCGGCCCCTCGCCAATGATCGCCAGCTGCAAGCCCGGTTGCCGTGCACTGGCCCTGGCGAATGCCTCGATCAGCATTTC  >  NC_002947/5619539‑5619828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑