Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,635,841 G→A 6.8% G70D (GGC→GAC)  PP_4948 → acyl‑CoA dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,635,8410GA 6.8% 167.0 / 2.4 60G70D (GGC→GAC) PP_4948acyl‑CoA dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (29/26);  new base A (2/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

CTGACCCTGCGCCCGCGCTTCGCCGAGCACAGCCGTGAAACCTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGC  >  NC_002947/5635702‑5635988
                                                                                                                                           |                                                                                                                                                   
cTGACCCTGCGCCCGCGCTTCGCCGAGCACAGCCGTGAAACCTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGcc                                                                                                                                                   <  6:128231/142‑1 (MQ=255)
cTGACCCTGCGCCCGCGCTTCGCCGAGCACAGCCGTGAAACCTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGcc                                                                                                                                                   >  5:128231/1‑142 (MQ=255)
cTGACCCTGCGCCCGCGCTTCGCCGAGCACAGCCGTGAAACCTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGACCGCGcc                                                                                                                                              >  2:285877/1‑147 (MQ=255)
   aCCCTGCGCCCGCGCTTCGCCGAGCACAGCCGTGAAACCTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAg                                                                                                                                           <  7:69501/147‑1 (MQ=255)
              gcgcTTCGCCGAGCACAGCCGTGAAACCTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGa                                                                                                                                            <  7:17560/135‑1 (MQ=255)
              gcgcTTCGCCGAGCACAGCCGTGAAACCTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGa                                                                                                                                            >  8:17560/1‑135 (MQ=255)
                gcTTCGCCGAGCACAGCCGTGAAACCTTCGTTGCGGCACTGACCACCGCCCGCACCATGGCCGATAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGtt                                                                                                                            <  7:82868/149‑1 (MQ=255)
                     gCCGAGCACAGCCGTGAAACCTTCGATGCGGCACTGACAACCGACCGCACCATCGCCGAAAAGTATTTCGCCACTCACCACCGCACGGCCGACTAAAACGAAACACGCAACGTCCACGCCCGCGCCGAGCTGCTCCACGAAGTTAAgac                                                                                                                       >  7:284288/1‑147 (MQ=255)
                            acaGCCGTGAAACCTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTc                                                                                                                >  3:4996/1‑149 (MQ=255)
                                ccGTGAAACCTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATg                                                                                                            >  2:328646/1‑149 (MQ=255)
                                        ccTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGAt                                                                                                             <  4:80394/140‑1 (MQ=255)
                                        ccTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGAt                                                                                                             >  3:80394/1‑140 (MQ=255)
                                          ttCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGa                                                                                                                                            >  1:184580/1‑107 (MQ=255)
                                          ttCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGa                                                                                                                                            <  2:184580/107‑1 (MQ=255)
                                          ttCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCg                                                                                                   >  5:22099/1‑148 (MQ=255)
                                          ttCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCg                                                                                                   >  4:96403/1‑148 (MQ=255)
                                                gCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCgg                                                                                            >  8:152273/1‑149 (MQ=255)
                                                gCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGACCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCgg                                                                                            >  8:300797/1‑149 (MQ=255)
                                                 cGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGc                                                                                           <  3:281829/149‑1 (MQ=255)
                                                 cGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGc                                                                                           <  6:22099/149‑1 (MQ=255)
                                                   gCACTGACCACCGCCCGCACCATCGCCGCAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAAAGAAACACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAACTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCtt                                                                                         >  2:72552/1‑149 (MQ=255)
                                                         accaccGCCCGCACCATTGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCaa                                                                                   <  7:249252/149‑1 (MQ=255)
                                                         accaccGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCACCGAAGTTAATCCCGCCGTAGATGACTTCCTCGAAGCCTGCTTCCTaaa                                                                                   >  8:292195/1‑149 (MQ=255)
                                                           caccGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACg                                                                                 >  7:28342/1‑149 (MQ=255)
                                                                   gcACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCgg                                                                         >  7:304775/1‑149 (MQ=255)
                                                                    cACCATCGCCGAAAAGTATTTCTCCCCGCACTACCGCAATGCCGACGAAAACGAAACACGCTACGTGTACGACAGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACTCCAACCggg                                                                        <  1:285877/149‑1 (MQ=255)
                                                                       cATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACt                                                                     >  6:21205/1‑149 (MQ=255)
                                                                       cATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACt                                                                     >  4:154993/1‑149 (MQ=255)
                                                                         tCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTc                                                                   <  8:304775/149‑1 (MQ=255)
                                                                             cGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCgg                                                                         >  6:276034/1‑139 (MQ=255)
                                                                             cGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCgg                                                                         <  5:276034/139‑1 (MQ=255)
                                                                             cGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAgg                                                               >  3:42658/1‑149 (MQ=255)
                                                                              gAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCt                                                                                      <  5:210194/125‑1 (MQ=255)
                                                                              gAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCt                                                                                      >  6:210194/1‑125 (MQ=255)
                                                                                aaaGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTcg                                                            <  7:152273/149‑1 (MQ=255)
                                                                                aaaGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTcg                                                            <  1:328646/149‑1 (MQ=255)
                                                                                   gTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCgg                                                                         <  4:94678/133‑1 (MQ=255)
                                                                                   gTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCgg                                                                         >  3:94678/1‑133 (MQ=255)
                                                                                    tATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTcggcgg                                                        <  7:292195/149‑1 (MQ=255)
                                                                                         cGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACTTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATg                                                    <  4:4996/148‑1 (MQ=255)
                                                                                          gCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATg                                                    >  3:170768/1‑147 (MQ=255)
                                                                                            cccGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGc                                                >  7:253745/1‑149 (MQ=255)
                                                                                             ccGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCt                                               >  5:8222/1‑149 (MQ=255)
                                                                                                    aCCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGc                                        <  4:170768/149‑1 (MQ=255)
                                                                                                       gCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCTACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTg                                     <  4:42658/149‑1 (MQ=255)
                                                                                                       gCAAGGCCGACGAAAACGAACCACGCTACGTGGACGACCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTg                                     <  7:300797/149‑1 (MQ=255)
                                                                                                               gacgaAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGc                              >  4:291288/1‑148 (MQ=255)
                                                                                                                acgaAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCaa                            <  3:291288/149‑1 (MQ=255)
                                                                                                                  gaAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGc                          <  7:97862/149‑1 (MQ=255)
                                                                                                                           ccACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGc                 <  8:253745/149‑1 (MQ=255)
                                                                                                                            cACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCa                     <  1:219514/144‑1 (MQ=255)
                                                                                                                            cACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCa                     >  2:219514/1‑144 (MQ=255)
                                                                                                                                  aCGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTc          >  5:301174/1‑149 (MQ=255)
                                                                                                                                   cGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTACATCGCCCACTTc          >  3:210261/1‑148 (MQ=255)
                                                                                                                                     tGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTc          <  4:67392/146‑1 (MQ=255)
                                                                                                                                     tGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTc          >  3:67392/1‑146 (MQ=255)
                                                                                                                                     tGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAg       >  6:42793/1‑149 (MQ=255)
                                                                                                                                      ggACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTcc         <  7:285761/146‑1 (MQ=255)
                                                                                                                                      ggACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTcc         >  8:285761/1‑146 (MQ=255)
                                                                                                                                         cGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGgccg   <  5:21205/149‑1 (MQ=255)
                                                                                                                                          ggCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGgccgc  <  3:96403/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
CTGACCCTGCGCCCGCGCTTCGCCGAGCACAGCCGTGAAACCTTCGATGCGGCACTGACCACCGCCCGCACCATCGCCGAAAAGTATTTCGCCCCGCACAACCGCAAGGCCGACGAAAACGAACCACGCTACGTGGACGGCCGCGCCGAGCTGATCCCCGAAGTTAAGCCCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGC  >  NC_002947/5635702‑5635988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑