Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,637,130 C→T 6.4% Q500* (CAA→TAA)  PP_4948 → acyl‑CoA dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,637,1300CT 6.4% 185.1 / 2.1 62Q500* (CAA→TAA) PP_4948acyl‑CoA dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (25/33);  new base T (2/2);  total (27/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CTCGGCCGCAAGCTGGCACAGCACAATGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGGCTGGCGCTGGCTGGAACAGGCGGTTCACGCCGAGCTTGGCAT  >  NC_002947/5636989‑5637278
                                                                                                                                             |                                                                                                                                                    
cTCGGCCGCAAGCTGGCACAGCACAATGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGc                                                                                                                                                 <  5:155024/147‑1 (MQ=255)
   ggCCGCAAGCTGGCACAGCACAATGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTaa                                                                                                                                             <  3:43726/148‑1 (MQ=255)
    gCCGCAAGCTGGCACAGCACAATGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCACCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACTGCCTGCAAGGCGTAAcc                                                                                                                                           >  1:258769/1‑149 (MQ=255)
     ccGCAAGCTGGCACAGCACAATGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAAccc                                                                                                                                          >  6:147548/1‑149 (MQ=255)
      cGCAAGCTGGCACAGCACAATGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCt                                                                                                                                         <  5:258480/149‑1 (MQ=255)
        cAAGCTGGCACAGCACAATGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTgg                                                                                                                                       >  7:180193/1‑149 (MQ=255)
        cAAGCTGGCACAGCACAATGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTgg                                                                                                                                       <  6:67235/149‑1 (MQ=255)
         aaGCTGGCACAGCACAATGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGc                                                                                                                                      <  1:101972/149‑1 (MQ=255)
                  cagcacaATGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACTGCCTGCAAGGCGTAACCCTGGCCCTTCTCgg                                                                                                                             <  2:258769/149‑1 (MQ=255)
                   agcacaATGGTGCCGGCCTCAAGCAATTGTTCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGc                                                                                                                            >  4:235060/1‑149 (MQ=255)
                        aaTGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCt                                                                                                                       >  4:294814/1‑149 (MQ=255)
                            gTGCCGTCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGATCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGc                                                                                                                    <  2:287578/148‑1 (MQ=255)
                                cGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAg                                                                                                               <  8:276218/149‑1 (MQ=255)
                                cGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAg                                                                                                               <  1:248845/149‑1 (MQ=255)
                                  gCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGccctggt                                                                                                              >  1:281417/1‑144 (MQ=255)
                                       aaGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGCCCTGGCCCAAGGCAAAGt                                                                                                        >  2:127082/1‑149 (MQ=255)
                                        aGCAATTGTTCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTc                                                                                                       <  3:235060/149‑1 (MQ=255)
                                        aGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAg                                                                                                               <  2:44770/141‑1 (MQ=255)
                                        aGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAg                                                                                                               >  1:44770/1‑141 (MQ=255)
                                         gCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCCCCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCg                                                                                                      >  5:90738/1‑149 (MQ=255)
                                         gCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCg                                                                                                      >  7:258833/1‑149 (MQ=255)
                                                aTCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCaa                                                                                                                >  3:169114/1‑132 (MQ=255)
                                                aTCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCaa                                                                                                                <  4:169114/132‑1 (MQ=255)
                                                   cGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCt                                                                                            <  8:258833/149‑1 (MQ=255)
                                                    gCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTg                                                                                           <  8:180193/149‑1 (MQ=255)
                                                        gATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGcc                                                                                        >  5:207126/1‑148 (MQ=255)
                                                            gccgccACCTGCGAGCGCTCCAGCCATCACCCTAACCTTGACCCACTTCGTCCGCCGATGGAGCAACTGGCCAACCGCCTCCAAGTCGTAAACCTGGCCCAGCTAGCCGACCTGGCCCAAGGAAAAGACGCTCGTGCCCAGGCCAActa                                                                                   >  6:312155/1‑148 (MQ=255)
                                                               gccACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGc                                                                                >  6:320404/1‑149 (MQ=255)
                                                               gccACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGc                                                                                >  6:315801/1‑149 (MQ=255)
                                                                    cTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGTCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGt                                                                           <  6:90738/149‑1 (MQ=255)
                                                                    cTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGt                                                                           <  8:175316/149‑1 (MQ=255)
                                                                    cTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGt                                                                           <  5:147548/149‑1 (MQ=255)
                                                                    cTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGt                                                                           <  5:315801/149‑1 (MQ=255)
                                                                    cTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGt                                                                           <  5:320404/149‑1 (MQ=255)
                                                                      gCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTAc                                                                         >  3:182223/1‑149 (MQ=255)
                                                                         aGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTc                                                                      <  6:207126/149‑1 (MQ=255)
                                                                            gcgcCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAg                                                                   <  7:210883/149‑1 (MQ=255)
                                                                              gccagccaTCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGc                                                                 >  8:259035/1‑149 (MQ=255)
                                                                                cagccaTCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCt                                                               >  6:280302/1‑149 (MQ=255)
                                                                                   ccaTCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCg                                                            <  1:127082/149‑1 (MQ=255)
                                                                                    caTCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCgg                                                           <  5:280302/149‑1 (MQ=255)
                                                                                       cACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCATGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCa                                                        >  6:298894/1‑149 (MQ=255)
                                                                                        aCCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTg                                                                            <  2:58347/128‑1 (MQ=255)
                                                                                        aCCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTg                                                                            >  1:58347/1‑128 (MQ=255)
                                                                                         cccGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACt                                                      <  7:259035/149‑1 (MQ=255)
                                                                                          ccGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTg                                                     >  3:32507/1‑149 (MQ=255)
                                                                                           cGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGc                                                    >  2:154146/1‑149 (MQ=255)
                                                                                             aaCCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGt                                                  <  3:294814/149‑1 (MQ=255)
                                                                                                        aCTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCtt                                                                             >  7:168016/1‑111 (MQ=255)
                                                                                                        aCTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCtt                                                                             <  8:168016/111‑1 (MQ=255)
                                                                                                            cGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGgctggcgct                                    <  4:182223/148‑1 (MQ=255)
                                                                                                             gTCAGCCGCTGGAGCAACTGGCCAACCGCCTGTAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGgctggcgc                                     <  6:49237/146‑1 (MQ=255)
                                                                                                             gTCAGCCGCTGGAGCAACTGGCCAACCGCCTGTAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGgctggcgc                                     <  6:44298/146‑1 (MQ=255)
                                                                                                             gTCAGCCGCTGGAGCAACTGGCCAACCGCCTGTAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGgctggcgc                                     >  5:44298/1‑146 (MQ=255)
                                                                                                             gTCAGCCGCTGGAGCAACTGGCCAACCGCCTGTAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGgctggcgc                                     >  5:49237/1‑146 (MQ=255)
                                                                                                             gTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGgctggcgctgg                                  <  1:154146/149‑1 (MQ=255)
                                                                                                                          gCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGGCTGGCGCTGGCTGGAACAGGCGG                     <  3:147465‑M1/149‑1 (MQ=255)
                                                                                                                             aCTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCATAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGGCTGGCGCTGGCTGGAACAGGCGGTTC                  <  1:181842‑M1/149‑1 (MQ=255)
                                                                                                                                   cAACCGCATGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGGCTGGCGCTGGCTGGAACAGGCGGTTCACGcc             >  2:156965/1‑148 (MQ=255)
                                                                                                                                    aaCCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCATGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGGCTGGCGCTGGCTGGAACAGGCGGTTCACGCCGa           <  5:298894/149‑1 (MQ=255)
                                                                                                                                          cTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCt                                                                              >  2:84217/1‑76 (MQ=255)
                                                                                                                                          cTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCt                                                                              <  1:84217/76‑1 (MQ=255)
                                                                                                                                             cAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGGCTGGCGCTGGCTGGAACAGGCGGTTCACGCCGAGCTTGGCAt  >  8:159891/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
CTCGGCCGCAAGCTGGCACAGCACAATGGTGCCGGCCTCAAGCAATTGATCCGCCTGATCGCCGCCACCTGCGAGCGCGCCAGCCATCACCCGAACCTTGACCCACTGCGTCAGCCGCTGGAGCAACTGGCCAACCGCCTGCAAGGCGTAACCCTGGCCCTGCTCGGCGACCTGGCCCAAGGCAAAGTCGCTGGTGCCCTGGCCAACTCTGCCTTGTACCTCAAGGCCTTCGGCCACTGCGTGATCGGCTGGCGCTGGCTGGAACAGGCGGTTCACGCCGAGCTTGGCAT  >  NC_002947/5636989‑5637278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑