Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,651,377 C→A 12.1% intergenic (‑185/+28) PP_4959 ← / ← fba diguanylate cyclase/fructose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,651,3770CA12.1% 178.2 / 13.0 66intergenic (‑185/+28)PP_4959/fbadiguanylate cyclase/fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (30/27);  new base A (3/5);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

TCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGAT  >  NC_002947/5651235‑5651521
                                                                                                                                              |                                                                                                                                                
tCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCggg                                                                                                                                            <  8:193899/149‑1 (MQ=255)
   aTTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGttt                                                                                                                                         >  3:203661/1‑149 (MQ=255)
       gTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGttttttt                                                                                                                                     <  2:28054/149‑3 (MQ=255)
         aCTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAg                                                                                                                                    <  7:187804/148‑1 (MQ=255)
         aCTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGttttttta                                                                                                                                    <  8:207622/148‑4 (MQ=255)
          ctctAGACCTGAGGCTGCAACACGCAATGCAATCAGACCGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGtttttttat                                                                                                                                   <  6:89152/148‑5 (MQ=255)
          ctctAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAggg                                                                                                                                  >  3:123523/1‑149 (MQ=255)
           tctAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCgg                                                                                                                                             <  6:206104/137‑1 (MQ=255)
           tctAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCgg                                                                                                                                             >  5:206104/1‑137 (MQ=255)
             tAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTa                                                                                                                               >  2:299786/1‑149 (MQ=255)
               gACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAcc                                                                                                                             <  3:174544/149‑1 (MQ=255)
               gACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAcc                                                                                                                             >  2:97983/1‑149 (MQ=255)
                 ccTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAc                                                                                                                              <  3:165946/146‑1 (MQ=255)
                 ccTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAc                                                                                                                              >  4:165946/1‑146 (MQ=255)
                  cTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAc                                                                                                                              >  3:79088/1‑145 (MQ=255)
                  cTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAc                                                                                                                              <  4:79088/145‑1 (MQ=255)
                     aGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTc                                                                                                                         <  8:92024/147‑1 (MQ=255)
                           cAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGtt                                                                                                                 <  8:125042/149‑1 (MQ=255)
                            aaCACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGttttttta                                                                                                                                    <  3:105437/129‑4 (MQ=255)
                            aaCACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGttttttta                                                                                                                                    >  4:105437/1‑126 (MQ=255)
                              cacGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGAc                                                                                                              <  1:299786/149‑1 (MQ=255)
                                   aatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTgg                                                                                                         >  8:172721/1‑149 (MQ=255)
                                       caatCAGAACGCTATCAATGATCAAACTGTACGGTATCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTggcggc                                                                                                     <  3:238813/149‑1 (MQ=255)
                                                 gCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCt                                                                                           >  2:307668/1‑149 (MQ=255)
                                                     tCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCGATCTCCCATAAAAATACCCGCATCTGTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCa                                                                                       <  7:108337/149‑1 (MQ=255)
                                                     tCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTc                                                                                        <  1:307668/148‑1 (MQ=255)
                                                     tCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCa                                                                                       <  4:203661/149‑1 (MQ=255)
                                                      cAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTCTCAGGGTTACCTGTCGATCa                                                                                                                    <  2:40849/119‑1 (MQ=255)
                                                      cAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTCTCAGGGTTACCTGTCGATCa                                                                                                                    >  1:40849/1‑119 (MQ=255)
                                                        aTGATCAAACTGTACGGTAGCCGACAAACGGTCGGTCTGAAGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACATGTCGATCAGTTTACCTTGGCGGCCAGTTCACCTTTCAgg                                                                                     >  1:65348/1‑148 (MQ=255)
                                                           aTCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAgc                                                                                 >  1:257314/1‑149 (MQ=255)
                                                            tCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTAAGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGTCTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGTCCAGTTCCCCTTTCAGGTAgcg                                                                                >  8:303156/1‑149 (MQ=255)
                                                             cAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAgcgc                                                                               >  5:103374/1‑149 (MQ=255)
                                                               aaCTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGAACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTg                                                                             >  7:171002/1‑149 (MQ=255)
                                                                aCTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTgg                                                                            <  1:97983/149‑1 (MQ=255)
                                                                  tGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGt                                                                           >  8:308220/1‑148 (MQ=255)
                                                                           gCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTc                                                                 <  7:172721/149‑1 (MQ=255)
                                                                           gCCGACTAACCGTCGGTCTGACTGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCtt                                                                  <  8:249054/148‑1 (MQ=255)
                                                                            ccGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTc                                                                 >  3:311279/1‑148 (MQ=255)
                                                                                cTAAGGTTCGGTCTGCCGTAATTATGAACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGTTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCagag                                                            <  8:171002/149‑1 (MQ=255)
                                                                                              gCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATc                                              >  3:296221/1‑149 (MQ=255)
                                                                                              gCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATc                                              >  2:254021/1‑149 (MQ=255)
                                                                                               ccGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCTCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCATTTCACCTTTCAGGTAGCGGTGGTACATGCCTTCCAGAGAGATCGGCTTGa                                                <  2:65348/146‑1 (MQ=255)
                                                                                               ccGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCt                                             >  4:255991/1‑149 (MQ=255)
                                                                                                 ggAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTc                                           <  2:257314/149‑1 (MQ=255)
                                                                                                  gAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCg                                          <  5:184469/149‑1 (MQ=255)
                                                                                                  gAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCAGAATCTCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGAAGCCCTAGTACATGCCTTAAATAGAGCTCCTCTTGATATTc                                           >  4:275071/1‑148 (MQ=255)
                                                                                                          tACAGGTTTCCAGCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCTCCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTg                                  <  4:218033/149‑1 (MQ=255)
                                                                                                               gTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCcgc                             >  4:252884/1‑149 (MQ=255)
                                                                                                                tttCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGccc                               <  1:298486/146‑1 (MQ=255)
                                                                                                                tttCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGccc                               >  2:298486/1‑146 (MQ=255)
                                                                                                                    cATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGc                        <  7:308220/149‑1 (MQ=255)
                                                                                                                     aTCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCa                                                                                                   >  2:218484/1‑73 (MQ=255)
                                                                                                                     aTCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCa                                                                                                   <  1:218484/73‑1 (MQ=255)
                                                                                                                        tcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCg                      >  4:308832/1‑147 (MQ=255)
                                                                                                                         cccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCATGTAGCGCTGGTACATGCCTTCCaga                                                             <  7:48396/107‑1 (MQ=255)
                                                                                                                         cccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAg                   <  3:255991/149‑1 (MQ=255)
                                                                                                                         cccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAAGGTTACCTATCGATCAGTTGACATTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCAaga                                                             >  8:48396/1‑107 (MQ=255)
                                                                                                                          ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAgg                  >  1:175295/1‑149 (MQ=255)
                                                                                                                               gaaaaaCCCGCAGCGATGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCg             >  5:68043/2‑149 (MQ=255)
                                                                                                                                aaaaaCCCGCAGCGATGCGGGTTTTTTAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGaa                    >  3:253185/1‑141 (MQ=255)
                                                                                                                                aaaaaCCCGCAGCGATGCGGGTTTTTTAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGaa                    <  4:253185/141‑1 (MQ=255)
                                                                                                                                  aaaCCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTaa          >  5:2974/1‑149 (MQ=255)
                                                                                                                                      ccGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAcgcg      >  7:232983/1‑149 (MQ=255)
                                                                                                                                       cGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAcgcgc     >  2:288268/1‑149 (MQ=255)
                                                                                                                                          aTCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGAt  <  2:100391/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
TCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGAT  >  NC_002947/5651235‑5651521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑