Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,660,182 G→T 8.6% intergenic (+35/‑194) metK → / → ligB methionine adenosyltransferase/DNA ligase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,660,1820GT 8.6% 135.5 / 2.3 45intergenic (+35/‑194)metK/ligBmethionine adenosyltransferase/DNA ligase B
Reads supporting (aligned to +/- strand):  ref base G (17/24);  new base T (2/2);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01

TTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGC  >  NC_002947/5660040‑5660323
                                                                                                                                              |                                                                                                                                             
ttCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCg                                                                                                                                         >  3:202301/1‑149 (MQ=255)
ttCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCCGCCGAGCGATCg                                                                                                                                         >  6:71334/1‑149 (MQ=255)
   ggCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCgg                                                                                                                                        >  4:76423/1‑147 (MQ=255)
     ccGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAgg                                                                                                                                    >  3:270901/1‑149 (MQ=255)
     ccGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACAACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAgg                                                                                                                                    <  4:175918/149‑1 (MQ=255)
      cGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAggg                                                                                                                                   <  3:287645/149‑1 (MQ=255)
       gTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGATCGCTCGGCAgg                                                                                                                                    <  8:28952/147‑1 (MQ=255)
       gTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACAGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGATCGCTCGGCAgg                                                                                                                                    >  7:28952/1‑147 (MQ=255)
            ccGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCtttt                                                                                                                             <  4:202301/149‑1 (MQ=255)
             cGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCg                                                                                                                                         <  2:25972/136‑1 (MQ=255)
             cGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCtttt                                                                                                                             <  6:22322/148‑1 (MQ=255)
             cGCAGCAGAAGACTGTCGGCGACGACACCATCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCg                                                                                                                                         >  1:25972/1‑136 (MQ=255)
               cagcagAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTtg                                                                                                                          <  2:210975/149‑1 (MQ=255)
                         cTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTa                                                                                                                 <  6:158817/148‑1 (MQ=255)
                                 gacgacACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGTGCTTTTTTGTGCCTGTTACGGCAgttt                                                                                                        >  8:260303/1‑149 (MQ=255)
                                   cgacACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCa                                                                                                      <  7:100386/149‑1 (MQ=255)
                                     acacCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATc                                                                                                    <  1:142195/149‑1 (MQ=255)
                                       accttcaccaccttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGATCGCTCGGCAggg                                                                                                                                   >  1:242656/1‑116 (MQ=255)
                                       accttcaccaccttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGATCGCTCGGCAggg                                                                                                                                   <  2:242656/116‑1 (MQ=255)
                                       accttcaccaccttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGc                                                                                                  >  6:268510/1‑149 (MQ=255)
                                                  cttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCg                                                                                       <  6:217347/149‑1 (MQ=255)
                                                     caccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAg                                                                                     <  4:120777/148‑1 (MQ=255)
                                                      accTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAgcg                                                                                   >  7:112407/1‑149 (MQ=255)
                                                        cTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGcgcc                                                                                 >  3:268931/1‑149 (MQ=255)
                                                          gggAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCt                                                                                                                     <  1:280463/111‑1 (MQ=255)
                                                          gggAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCt                                                                                                                     >  2:280463/1‑111 (MQ=255)
                                                           gtcacgcAACGACTGGGCGCGGTCGGTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCcgcg                                                                              >  7:177727/5‑149 (MQ=255)
                                                                gcACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAg                                                                                                                                               <  8:27676/79‑1 (MQ=255)
                                                                gcACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAg                                                                                                                                               >  7:27676/1‑79 (MQ=255)
                                                                gcACCGACCGCGCCCAGTCGTTGCGTGACGCTACCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGt                                                                         <  5:234269/149‑1 (MQ=255)
                                                                      accgCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTg                                                                   <  4:270901/149‑1 (MQ=255)
                                                                               aGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGtctc                                                          <  5:268510/149‑1 (MQ=255)
                                                                                gTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGTGCGCCGCGCTCGTTTCTTGCTCCGTCTCg                                                         <  1:192546/149‑1 (MQ=255)
                                                                                               tGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTT‑TTCCAGTTAAGGCAATTTACTAGCCCGGCAGAAGGAGCGGCGCGCTAGTTTATTTGAGCGTGTAGGGGTTTGAGACGAGc                                          >  5:78987/1‑148 (MQ=255)
                                                                                                 ccGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTTCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCa                                         <  5:320285/148‑1 (MQ=255)
                                                                                                   ggCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCa                                      <  4:268931/149‑1 (MQ=255)
                                                                                                   caggtGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCa                                      <  8:219257/145‑1 (MQ=255)
                                                                                                         tAAGCTTCCTGCAGCGCCAGACGAAAGCCCCCGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGaa                                 <  5:71334/148‑1 (MQ=255)
                                                                                                             cTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATc                            <  7:43164/149‑1 (MQ=255)
                                                                                                                ccTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCAc                          <  8:112407/148‑1 (MQ=255)
                                                                                                                  tGCAGAGCAAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCTCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCACCGAAatataccct                        >  3:204544/1‑143 (MQ=255)
                                                                                                                    cAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGa                                            >  6:277009/1‑126 (MQ=255)
                                                                                                                    cAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGa                                            <  5:277009/126‑1 (MQ=255)
                                                                                                                             aCGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCt                        <  4:170982/137‑1 (MQ=255)
                                                                                                                             aCGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCt                        >  3:170982/1‑137 (MQ=255)
                                                                                                                                     cccTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGGAGCGTCTCGGGCTTGGAGACGAGGACCAGGGATCTATCACATGACACCTGCACGACccgccc    >  8:256760/1‑149 (MQ=255)
                                                                                                                                       cTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCAccgcccgc  >  6:192198/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                             
TTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGC  >  NC_002947/5660040‑5660323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑