Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,685,911 G→C 6.4% R328G (CGC→GGC) ‡ PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,685,9110GC 6.4% 240.6 / 4.1 74R328G (CGC→GGC) ‡PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base G (35/34);  new base C (3/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTCCTCCAAA  >  NC_002947/5685770‑5686053
                                                                                                                                             |                                                                                                                                              
tCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGCCCTTGCCgg                                                                                                                                         <  4:291008/149‑1 (MQ=255)
  ggcggcTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCgggg                                                                                                                                       <  5:64348/149‑1 (MQ=255)
   gcggcTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGc                                                                                                                                      <  1:147730/149‑1 (MQ=255)
     ggcTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGc                                                                                                                                      >  2:45794/1‑147 (MQ=255)
          ttCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTc                                                                                                                                 <  4:315471/147‑1 (MQ=255)
              tccAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGATTCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTc                                                                                                                           >  7:278637/1‑149 (MQ=255)
               ccAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCAACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTc                                                                                                                           >  3:23761/1‑148 (MQ=255)
                cAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTcc                                                                                                                          <  7:97252/148‑1 (MQ=255)
                cAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTc                                                                                                                           >  3:231362/1‑147 (MQ=255)
                 aGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCAACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAg                                                                                                                        <  4:23761/149‑1 (MQ=255)
                  ggTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAgg                                                                                                                       <  3:247565/149‑1 (MQ=255)
                        gcAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAAACg                                                                                                                 >  3:136309/1‑149 (MQ=255)
                        gcAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCCGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTc                                                                                                                                 >  5:210418/1‑133 (MQ=255)
                        gcAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCCGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTc                                                                                                                                 <  6:210418/133‑1 (MQ=255)
                           accaacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGcc                                                                                                               >  1:148308/1‑148 (MQ=255)
                             caacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGAt                                                                                                            >  5:320973/1‑149 (MQ=255)
                             caacGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGAt                                                                                                            <  4:231362/149‑1 (MQ=255)
                                  cTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCACGCGGATCTGCTGCAACAGCCACGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGAt                                                                                                            >  6:249238/1‑144 (MQ=255)
                                      acaTAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCtt                                                                                                   >  2:212719/1‑149 (MQ=255)
                                       caTAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTg                                                                                                  <  6:320973/149‑1 (MQ=255)
                                        aTAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAAt                                                                                                                   <  8:98724/131‑1 (MQ=255)
                                        aTAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAAt                                                                                                                   >  7:98724/1‑131 (MQ=255)
                                        aTAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGa                                                                                                             >  6:8460/1‑137 (MQ=255)
                                        aTAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGa                                                                                                             <  5:8460/137‑1 (MQ=255)
                                        aTAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTgg                                                                                                 >  6:318272/1‑149 (MQ=255)
                                            cTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGc                                                                                                                                            <  3:239705/102‑1 (MQ=255)
                                            cTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGc                                                                                                                                            >  4:239705/1‑102 (MQ=255)
                                               gcgcAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGATTCCAGGTAATCGCCGATCAGATCCTTGGCctgct                                                                                           <  1:45794/148‑1 (MQ=255)
                                                cgcAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCctgctg                                                                                          >  2:68886/1‑148 (MQ=255)
                                                   aGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCATTGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAac                                                                                      >  1:231092/1‑149 (MQ=255)
                                                    gCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAaca                                                                                     >  8:6019/1‑149 (MQ=255)
                                                      cGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACg                                                                                   <  5:318272/149‑1 (MQ=255)
                                                       gACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGt                                                                                  >  4:181955/1‑149 (MQ=255)
                                                          aaaCAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGCCCTTGCCgg                                                                                                                                         >  8:116157/1‑91 (MQ=38)
                                                           atgctgccGGCCGTGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAg                                                                              >  8:276601/8‑149 (MQ=255)
                                                           aaCAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAg                                                                              >  1:21959/1‑149 (MQ=255)
                                                           aaCAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAg                                                                              <  1:68886/149‑1 (MQ=255)
                                                            aCAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGc                                                                             >  5:190292/1‑149 (MQ=255)
                                                            aCAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGc                                                                             >  7:181109/1‑149 (MQ=255)
                                                              aGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACt                                                                                                                            >  5:233973/1‑100 (MQ=255)
                                                              aGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACt                                                                                                                            <  6:233973/100‑1 (MQ=255)
                                                                       aCGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAg                                                                  >  2:85998/1‑149 (MQ=255)
                                                                                agcaTCAAGATGGCCATTGCCCTGCGGATCTGCTGGAACAGCCCCGGCAAGGGCTGCTGGCGTTGCCGGGGCCAGTCTGACTCCAGTTAATCGCCGATCAGATGCTTGGCCTTTTGCAACTCGTTCTGCGATTCATTCAGCACCAGCTg                                                         <  4:136309/149‑1 (MQ=255)
                                                                                         aTGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTAtctc                                                >  8:283998/1‑149 (MQ=255)
                                                                                            gCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGc                                             <  3:181955/149‑1 (MQ=255)
                                                                                               aaTGCACCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAg                                          <  1:193697/149‑1 (MQ=255)
                                                                                                aTGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAgg                                         >  7:54158/1‑149 (MQ=255)
                                                                                                      cgcgGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAg                                   >  6:91978/1‑149 (MQ=255)
                                                                                                      cgcgGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACCCCAGGTAATCGCCGGTCAGATCCTTgg                                                                                                 >  2:150287/1‑87 (MQ=255)
                                                                                                      cgcgGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACCCCAGGTAATCGCCGGTCAGATCCTTgg                                                                                                 <  1:150287/87‑1 (MQ=255)
                                                                                                            tCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCgg                              >  3:149265/1‑148 (MQ=255)
                                                                                                                ctgcAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCa                            <  2:21959/146‑1 (MQ=255)
                                                                                                                catcAACAGCCCCGTCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAgg                          <  8:278637/145‑1 (MQ=255)
                                                                                                                 tgcAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCt                        >  2:172615/1‑149 (MQ=255)
                                                                                                                   cAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCt                        <  1:172615/147‑1 (MQ=255)
                                                                                                                   cAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGCATCTCGGCCAGGTCCGAGCCCGGCAGGCt                        <  7:6019/147‑1 (MQ=255)
                                                                                                                   cAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCAATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGc                      <  2:231092/149‑1 (MQ=255)
                                                                                                                    aaCAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGcc                     <  5:249238/149‑1 (MQ=255)
                                                                                                                      cAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCGACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCtt                   <  1:212719/149‑1 (MQ=255)
                                                                                                                        gCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTATTCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCCGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTgg                 <  6:190292/149‑1 (MQ=255)
                                                                                                                        gCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTgg                 >  8:292004/1‑149 (MQ=255)
                                                                                                                          cccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGcc               <  7:292004/149‑1 (MQ=255)
                                                                                                                          cccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGcc               >  6:267603/1‑149 (MQ=255)
                                                                                                                          cccGGCAACGCCTGCAGCCCTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGcc               >  1:142171/1‑149 (MQ=255)
                                                                                                                          cccGGCAACGCCTGCAGCCCTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGAGTCATTCAGCCCCAGCTGGCGTATCTCCGCCAGGTCCGCGCCCGGCAGGCTGCCTTGGcc               >  5:59405/1‑149 (MQ=255)
                                                                                                                           ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGc                >  7:189689/1‑147 (MQ=255)
                                                                                                                           ccGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGc                <  8:189689/147‑1 (MQ=255)
                                                                                                                              gCAACGCCTGCAGCCCTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGttt           <  2:142171/149‑1 (MQ=255)
                                                                                                                                 aGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTtcc        <  8:54158/149‑1 (MQ=255)
                                                                                                                                   ggCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTtcctc      <  2:148308/149‑1 (MQ=255)
                                                                                                                                     cTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTtcctcc     >  4:174289/1‑148 (MQ=255)
                                                                                                                                        cAGGCGTTGCCGGTGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTtcct       <  2:112447/143‑1 (MQ=255)
                                                                                                                                        cAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTtcct       >  1:112447/1‑143 (MQ=255)
                                                                                                                                        cAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTCCTCCaaa  <  7:276601/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                              
TCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTTTCCTCCAAA  >  NC_002947/5685770‑5686053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑