Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,711,857	G→A	11.3%	E232E (GAG→GAA)	ubiE →	ubiquinone/menaquinone biosynthesis C‑methyltransferase UbiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,711,857	0	G	A	11.3%	165.7 / 8.8	62	E232E (GAG→GAA)	ubiE	ubiquinone/menaquinone biosynthesis C‑methyltransferase UbiE
Reads supporting (aligned to +/- strand):  ref base G (28/27);  new base A (2/5);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

TGATGTCCAAGGCCTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACG  >  NC_002947/5711709‑5712000                                                                                                                                                     |                                                                                                                                                tGATGTCCAAGGCCTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAg                                                                                                                                                 <  1:138291/149‑1 (MQ=255)   aTGTCCAAGGCCTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAg                                                                                                                                                 <  5:151320/147‑1 (MQ=255)   aTGTCCAAGGCCTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGc                                                                                                                                               >  8:56412/1‑149 (MQ=255)        cAAGGCCTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAg                                                                                                                                                 >  3:147253/1‑142 (MQ=255)        cAAGGCCTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAg                                                                                                                                                 <  4:147253/142‑1 (MQ=255)                  gacgCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAAGCCGGCCTCGAccatca                                                                                                                                <  3:130525/149‑5 (MQ=255)                   acgCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAAGCCGGCCTCGAccatcat                                                                                                                               <  8:169306/149‑6 (MQ=255)                     gCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAAGCCGGCCTCGAccatcatgc                                                                                                                             <  6:240808/149‑8 (MQ=255)                         aCTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTAc                                                                                                                         >  3:173538/1‑149 (MQ=255)                           tCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACca                                                                                                                       >  6:299564/1‑149 (MQ=255)                             gTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACcac                                                                                                                      >  2:308077/1‑148 (MQ=255)                             gTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACcac                                                                                                                      >  7:263151/1‑148 (MQ=255)                             gTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAAGCCGGCCTCGAccatcatg                                                                                                                              >  1:97957/1‑134 (MQ=255)                             gTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAAGCCGGCCTCGAccatcatg                                                                                                                              <  2:102005/140‑7 (MQ=255)                             gTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAAGCCGGCCTCGAccatcatg                                                                                                                              <  2:97957/140‑7 (MQ=255)                             gTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAAGCCGGCCTCGAccatcatg                                                                                                                              >  1:102005/1‑134 (MQ=255)                              ttCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCacaa                                                                                                                    <  3:320113/149‑1 (MQ=255)                               tCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCt                                                                                                                           <  5:88448/141‑1 (MQ=255)                               tCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCt                                                                                                                           >  6:88448/1‑141 (MQ=255)                                 gCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCacaaca                                                                                                                  <  2:144581/148‑1 (MQ=255)                                 gCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACAt                                                                                                                 >  4:124906/1‑149 (MQ=255)                                   cTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCacaac                                                                                                                   >  7:92331/1‑145 (MQ=255)                                   cTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCacaac                                                                                                                   <  8:92331/145‑1 (MQ=255)                                        tGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGc                                                                                                          >  4:19560/1‑149 (MQ=255)                                         gCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGa                                                                                                                                      >  4:233348/1‑120 (MQ=255)                                         gCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGa                                                                                                                                      <  3:233348/120‑1 (MQ=255)                                          ccGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCg                                                                                                         <  4:173538/148‑1 (MQ=255)                                                 ctggcAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGtt                                                                                                 <  5:299564/149‑1 (MQ=255)                                                                  aaTGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGAt                                                                                <  7:56412/149‑1 (MQ=255)                                                                    tGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATc                                                                               >  6:162234/1‑148 (MQ=255)                                                                        gCCGAGAGTTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCg                                                                                             <  6:224989/130‑1 (MQ=255)                                                                        gCCGAGAGATACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCg                                                                                             >  5:224989/1‑130 (MQ=255)                                                                             gagCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGAt                                                                     <  5:162234/149‑1 (MQ=255)                                                                               gCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGt                                                                   <  4:263502/149‑1 (MQ=255)                                                                                  aCCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCAGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGc                                                                >  1:92242/1‑149 (MQ=255)                                                                                   ccGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGc                                                                <  8:263151/148‑1 (MQ=255)                                                                                   ccGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCt                                                               >  2:94446/1‑149 (MQ=255)                                                                                    cGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTg                                                              <  1:94446/149‑1 (MQ=255)                                                                                           tCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGgc                                                       >  1:280093/1‑149 (MQ=255)                                                                                             gCCGAGTCGATCCACATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGa                                                                      >  4:180759/1‑132 (MQ=255)                                                                                             gCCGAGTCGATCCACATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGa                                                                      <  3:180759/132‑1 (MQ=255)                                                                                                gAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTc                                                  >  3:16090/1‑149 (MQ=255)                                                                                                         cGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTc                                         <  5:51256/149‑1 (MQ=255)                                                                                                         cGCATGCACCCGGACCAGGAAACCCTGAAGACCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGt                                          <  3:19560/148‑1 (MQ=255)                                                                                                          gCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTc                                         >  6:178874/1‑148 (MQ=255)                                                                                                           cATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCg                                        >  8:137479/1‑148 (MQ=255)                                                                                                           cATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGa                                       <  5:178874/149‑1 (MQ=255)                                                                                                              gCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACa                                    >  5:263189/1‑149 (MQ=255)                                                                                                               ttcccGGACCAGTAAACCCTGAATTCCATTTTGGGCGTGGCCGGCTTCCCCGGGGGATCCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAAc                                     <  2:258683/145‑1 (MQ=255)                                                                                                               cACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACAt                                   <  7:137479/149‑1 (MQ=255)                                                                                                                aCCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATg                                  >  6:172263/1‑149 (MQ=255)                                                                                                                 cccGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATgg                                 <  4:16090/149‑1 (MQ=255)                                                                                                                  ccGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAAc                                     >  1:258683/1‑144 (MQ=255)                                                                                                                  ccGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGc                                >  4:210683/1‑149 (MQ=255)                                                                                                                  ccGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGc                                >  1:55511/1‑149 (MQ=255)                                                                                                                   cGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGcc                               <  3:124906/149‑1 (MQ=255)                                                                                                                         aGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAAcc                         <  1:308077/149‑1 (MQ=255)                                                                                                                             aaCCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACcgcg                      >  1:319703/1‑148 (MQ=255)                                                                                                                                ccTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCt                  >  1:165092/1‑149 (MQ=255)                                                                                                                                 cTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCGAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTg                 >  5:92368/1‑149 (MQ=255)                                                                                                                                   gAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAAcc                         >  4:172182/1‑139 (MQ=255)                                                                                                                                   gAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAAcc                         <  3:172182/139‑1 (MQ=255)                                                                                                                                        gCATGATGGTCGAGGCCGGCTTCTACCGCGTCACCTTCCACAAGATGACCAGCGGGATCGTTGCCGGGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTgg          <  6:263189/149‑1 (MQ=255)                                                                                                                                        gCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTg           <  2:319703/148‑1 (MQ=255)                                                                                                                                         cttgatgGGCGTGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGa         <  2:165092/147‑1 (MQ=255)                                                                                                                                                gTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCGAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACg  <  6:92368/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                TGATGTCCAAGGCCTACGACGCCTACTCGTTCGCCTTCATGCCGCTGGCTGGCAAGCTGATCACCAATGACGCCGAGAGCTACCGTTACCTCGCCGAGTCGATCCGCATGCACCCGGACCAGGAAACCCTGAAGAGCATGATGGTCGAGGCCGGCTTCGACCGCGTCACCTACCACAACATGACCAGCGGCATCGTTGCCGTGCACCGGGGGATCAAGCCCTGATGTTGCTGGCCGGGCTGCTCGCCAGCGTCGAACATGGCCTGAACCGCGTCCTGCGCCTGGACAGCACG  >  NC_002947/5711709‑5712000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑