Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 571,618 T→C 9.3% intergenic (+44/+47) ssb → / ← PP_0486 single‑stranded DNA‑binding protein/GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947571,6180TC 9.3% 111.3 / 3.2 43intergenic (+44/+47)ssb/PP_0486single‑stranded DNA‑binding protein/GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (19/20);  new base C (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01

AGGCCCAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGA  >  NC_002947/571471‑571759
                                                                                                                                                    |                                                                                                                                             
agGCCCAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGtt                                                                                                                                              <  2:146650/149‑1 (MQ=255)
     cAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCt                                                                                                                                         <  6:216616/149‑1 (MQ=255)
       gcgcCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTgg                                                                                                                                       <  4:81194/149‑1 (MQ=255)
       gcgcCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAACTGCCCTgg                                                                                                                                       <  7:260598/149‑1 (MQ=255)
        cgcCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTTTGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAACTGCCCTggg                                                                                                                                      <  1:94401/149‑1 (MQ=255)
           ccGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGttt                                                                                                                                   >  4:314800/1‑149 (MQ=255)
             gCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCTTGGGttttt                                                                                                                                 <  5:2032/149‑1 (MQ=255)
                aCAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAATCCCAGGGCAGTTGCCCTGGGTTTTTTTa                                                                                                                              <  1:187240/149‑1 (MQ=255)
                    gCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGttttt                                                                                                                                 <  1:193777/142‑1 (MQ=255)
                    gCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGttttt                                                                                                                                 >  2:193777/1‑142 (MQ=255)
                            gcgcccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACcg                                                                                                                  >  2:127213/1‑149 (MQ=255)
                                   gcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTAt                                                                                                             >  5:41452/1‑147 (MQ=255)
                                       cgcAACAGCCTGCGCCGCTGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGTGTTTTTTTATGGGCAGCACCGCGTATCAg                                                                                                          <  1:263252/147‑1 (MQ=255)
                                       cgcAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGTGCAGCACCGCGAATCAg                                                                                                          >  2:263252/1‑146 (MQ=255)
                                              gCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCttt                                                                                                <  6:41452/149‑1 (MQ=255)
                                                cTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCa                                                                                              <  3:95748/149‑1 (MQ=255)
                                                cTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCa                                                                                              <  8:5946/149‑1 (MQ=255)
                                                cTGCGCCGCAGCCGGCGACTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCa                                                                                              <  8:13134/149‑1 (MQ=255)
                                                   cgccgcAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAg                                                                                             <  6:117984/147‑1 (MQ=255)
                                                    gccgccGGCGGGGGCGGTATTCGTCAGCTTCGTTTTCTTTATTCCGTTTTGATTATTGGGGTTGGG‑CGTTCTTTAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAg                                                                                          <  7:49288/149‑1 (MQ=255)
                                                          gCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGaacaac                                                                                    <  4:163200/149‑1 (MQ=255)
                                                           ccGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAAcc                                                                                   <  3:314800/149‑1 (MQ=255)
                                                                     aCTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCgg                                                                         >  7:97631/1‑149 (MQ=255)
                                                                                   gATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAgg                                                            <  1:202931/148‑1 (MQ=255)
                                                                                                 cgatctaTCATAGCGGTAGGG‑CGTTAAGCAAGAACAGAAACCCAGGGCAGTTGCCCTTGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGt                                              <  6:334992/142‑1 (MQ=255)
                                                                                                        tCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAAGCATCGCTTTCAGTATAACAACCTGGGCTACGGCGAATCCAGCAGGCTTGCAAGCTTGGTCAgatat                                       >  5:334992/1‑144 (MQ=255)
                                                                                                          agagCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAg                                    >  7:147691/1‑149 (MQ=255)
                                                                                                                   ggg‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCt                           >  2:23223/1‑149 (MQ=255)
                                                                                                                         ttCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCt                                                  <  2:209139/121‑1 (MQ=255)
                                                                                                                         ttCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCt                                                  >  1:209139/1‑121 (MQ=255)
                                                                                                                              cAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCAAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTgacgcatcg                 >  3:188548/1‑144 (MQ=255)
                                                                                                                                aGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGt               >  2:230567/1‑149 (MQ=255)
                                                                                                                                 gACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCtt                          >  4:300608/1‑137 (MQ=255)
                                                                                                                                 gACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCtt                          <  3:300608/137‑1 (MQ=255)
                                                                                                                                    cAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATcc                                                                  <  4:104507/94‑1 (MQ=255)
                                                                                                                                    cAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATcc                                                                  >  3:104507/1‑94 (MQ=255)
                                                                                                                                    cAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCa           >  8:307961/1‑149 (MQ=255)
                                                                                                                                      gAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCAt          >  7:121340/1‑148 (MQ=255)
                                                                                                                                       aaaCCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAATCCCAGTTCTTCGCGGCTGGGTGCCAt          >  4:44728/1‑147 (MQ=255)
                                                                                                                                        aaCCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAg       <  8:97631/149‑1 (MQ=255)
                                                                                                                                         aCCCAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAgg      >  3:122770/1‑149 (MQ=255)
                                                                                                                                          cccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGTTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAggg     >  6:277937/1‑149 (MQ=255)
                                                                                                                                           ccAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGc    >  1:199583/1‑149 (MQ=255)
                                                                                                                                             aGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGa  >  5:252728/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
AGGCCCAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGG‑CGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGA  >  NC_002947/571471‑571759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑