| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,740,471 | A→T | 23.9% | intergenic (+36/+35) | hutF → / ← PP_5037 | formiminoglutamate deiminase/lipocalin family lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,740,471 | 0 | A | T | 23.9% | 61.8 / 22.2 | 42 | intergenic (+36/+35) | hutF/PP_5037 | formiminoglutamate deiminase/lipocalin family lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (15/17); new base T (4/6); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-02 | |||||||||||
GTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGT > NC_002947/5740325‑5740619 | gTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGc < 3:268976/149‑1 (MQ=255) gcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATAGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAACCCTTCCC‑GTGCAAGcccc > 1:259385/1‑148 (MQ=255) gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAg < 5:137220/137‑1 (MQ=255) gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAg > 6:137220/1‑137 (MQ=255) gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAACGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGc < 6:269281/138‑1 (MQ=255) gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAACGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGc > 5:269281/1‑138 (MQ=255) aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGtt < 3:157541/149‑1 (MQ=255) aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GGGCTTGCACGCGAAgggc < 2:240189/148‑2 (MQ=255) aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAACCCTTCGC‑GGGCTTGCACGCGAAgggc < 2:16289/148‑2 (MQ=38) tGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTg > 8:150704/1‑149 (MQ=255) gTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAACCCTTCGC‑GGGCTTGCACGCGAAGGGCTgc < 8:173354/148‑1 (MQ=38) aaTGGGCAATGGGGGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTgcaac < 7:150704/148‑1 (MQ=255) gCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCcgc < 3:309237/130‑1 (MQ=255) gCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCAAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCcgc > 4:309237/1‑130 (MQ=255) tgCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTTGAGGCCCTAGC‑CGGGGAGCCCGCGAAGGGTTTGAACCGCACCCCaaaaaaaa > 2:276589/1‑146 (MQ=255) ccAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGGGGTTGC‑GCCCATAGCGGGGCAAGCCCCGCACGGGTTGGAACCCCACCCCaaaaaaaacctg > 3:315022/1‑143 (MQ=255) cAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAACCCTTCGC‑GGGCTTGCACGCGAAg < 7:36291/103‑1 (MQ=37) cAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAACCCTTCGC‑GGGCTTGCACGCGAAg > 8:36291/1‑103 (MQ=37) aaGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTG‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgc < 3:81467/149‑1 (MQ=255) gcgggcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCa < 8:238596/149‑1 (MQ=255) gcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAg > 6:280405/1‑82 (MQ=255) gcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAg < 5:280405/82‑1 (MQ=255) ttGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTc < 1:16493/148‑1 (MQ=255) cgcCAGGTTTTGGGCGGAGGGTACCGGGGTG‑GCGTTGCAGCCGTGGGC‑GTGCAAGCCGGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGTCGCCCGCCAGATCAAGCGGCTGGTGTCATa < 1:196623/148‑1 (MQ=255) ccAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTAGC‑GGGCAAGCCCGCGAAGGGTTGGCACGCCACCCCCAAAAACGCAGTGGATTTTTTCGATTTGCGGATCGGACGCCCGCCAGATCAAGCGGCGGGGGTCATAacc > 2:45521/1‑149 (MQ=255) cAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTgc > 2:316016/1‑67 (MQ=255) aGCTTTTGGGCTGAGCGTACCGGGGTG‑GCGTTCCAGCCCGTCGC‑GGCCAAGCCCGCGAAGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCct < 4:304944/149‑1 (MQ=255) ttttGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCACATCAAGCGGCTGGTGTCATACCCctgct < 1:187067/149‑1 (MQ=255) ttGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct < 2:259385/147‑1 (MQ=255) aGCGTACCGGGGTG‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGATGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt < 4:315022/149‑1 (MQ=255) gCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg < 1:45521/149‑1 (MQ=255) gtt‑gcgttgcAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGt > 2:127446/1‑149 (MQ=255) ttgcAACCCTTCGC‑GGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc > 5:120314/1‑149 (MQ=38) cAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGAAACCCCAAAAAACTCAGTGCAATTTTACTATTTGAAGAACGTACTCACGCAAGATAAAGCGGCTGGTGTCATACCCCTTCTGCCGCGCACTCGACAGCAGGTTCTCACGCt > 6:125138/1‑149 (MQ=255) gCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGCTCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc > 8:188150/1‑149 (MQ=255) cTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg > 4:231948/1‑149 (MQ=255) cTTCGC‑GGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGcc < 6:128440/117‑1 (MQ=38) cTTCGC‑GGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGcc > 5:128440/1‑117 (MQ=38) ttCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAgg > 3:6168/1‑149 (MQ=255) tCGC‑GGGCTTGCACGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt < 5:104851/122‑1 (MQ=255) tCGC‑GGGCTTGCACGCGAAGGGTTGCAAAGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt > 6:104851/1‑122 (MQ=39) tGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCa < 1:127446/149‑1 (MQ=255) aGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAgtgt > 7:165564/1‑149 (MQ=255) | GTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGAT‑GAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGT > NC_002947/5740325‑5740619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |