Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,790,159 A→T 7.0% intergenic (+38/+30) ynfM → / ← PP_5073 efflux transporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,790,1590AT 7.0% 171.2 / 2.0 57intergenic (+38/+30)ynfM/PP_5073efflux transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (29/24);  new base T (2/2);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTCGGCGGCAGG  >  NC_002947/5790013‑5790300
                                                                                                                                                  |                                                                                                                                             
cTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAg                                                                                                                                              <  6:134637/148‑1 (MQ=255)
 tGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCg                                                                                                                                            >  4:107626/1‑149 (MQ=255)
 tGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCg                                                                                                                                            >  2:310191/1‑149 (MQ=255)
   gCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGTCACACCCGCTCCCACAGGTTGTGGGTGc                                                                                                                                             <  4:95547/146‑1 (MQ=39)
   gCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCTCCCACAGGTTGTGGGTGc                                                                                                                                             <  7:65069/146‑1 (MQ=255)
   gCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCgg                                                                                                                                           <  7:146218/148‑1 (MQ=255)
                  gAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAagag                                                                                                                           >  5:236411/1‑149 (MQ=255)
                        gATCGGGCTTTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCatc                                                                                                                     <  7:28892/149‑1 (MQ=255)
                        gATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCat                                                                                                                      >  7:102311/1‑148 (MQ=255)
                        gATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCat                                                                                                                      <  8:102311/148‑1 (MQ=255)
                         aTCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCat                                                                                                                      >  4:206489/1‑147 (MQ=255)
                             ggCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCatcagat                                                                                                                 >  6:226677/1‑148 (MQ=255)
                             ggCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCatcagat                                                                                                                 >  6:231243/1‑148 (MQ=255)
                                 gTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGtt                                                                                                            >  6:310809/1‑149 (MQ=255)
                                     aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTtgat                                                                                                         <  5:310809/148‑1 (MQ=255)
                                         gCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGt                                                                                                             <  6:136728/140‑1 (MQ=255)
                                         gCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGAtct                                                                                                    >  3:257528/1‑149 (MQ=255)
                                         gCAGCTTGCTGGCGGTGGCCTAGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGt                                                                                                             >  5:136728/1‑140 (MQ=255)
                                            gCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGa                                                                                                 <  1:310191/149‑1 (MQ=255)
                                                 cTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTTATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTTACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAat                                                                                             <  5:167003/148‑1 (MQ=255)
                                                  tGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATCATCTCGACAatat                                                                                           >  1:21511/1‑149 (MQ=255)
                                                    gCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatc                                                                                          <  3:107626/148‑1 (MQ=255)
                                                      ggTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatccac                                                                                       <  7:79198/149‑1 (MQ=255)
                                                            cTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatccacatccac                                                                                 >  1:123195/1‑149 (MQ=255)
                                                                         ttGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCtcatc                                                                    <  5:226677/149‑1 (MQ=255)
                                                                         ttGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCtcat                                                                     >  2:175132/1‑148 (MQ=255)
                                                                             aCCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGt                                                                >  2:153920/1‑149 (MQ=255)
                                                                             aCCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGt                                                                <  2:123195/149‑1 (MQ=255)
                                                                             aCCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGt                                                                >  8:1888/1‑149 (MQ=255)
                                                                               cTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGc                                                              <  1:153920/149‑1 (MQ=255)
                                                                                        ttGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAAc                                                      >  4:34985/1‑148 (MQ=255)
                                                                                             aCCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAat                                                                                             <  7:86775/104‑1 (MQ=255)
                                                                                             aCCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAat                                                                                             >  8:86775/1‑104 (MQ=255)
                                                                                             aCCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCAcc                                                >  2:106816/1‑149 (MQ=255)
                                                                                             aCCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCAcc                                                >  2:15365/1‑149 (MQ=255)
                                                                                              cccAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAgg                                                                                                                          >  4:207374/1‑74 (MQ=255)
                                                                                              cccAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAgg                                                                                                                          <  3:207374/74‑1 (MQ=255)
                                                                                               ccAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGAGCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTAAACCTCACCgg                                              >  7:265166/1‑149 (MQ=255)
                                                                                                cAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGt                                             >  3:236352/1‑149 (MQ=255)
                                                                                                          tgATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCaa                                                       >  3:291112/1‑129 (MQ=255)
                                                                                                          tgATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCaa                                                       <  4:291112/129‑1 (MQ=255)
                                                                                                          tgATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCAc                                    <  4:257528/148‑1 (MQ=255)
                                                                                                             tGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTg                                <  7:140216/149‑1 (MQ=255)
                                                                                                             tGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTg                                <  5:231243/149‑1 (MQ=255)
                                                                                                                aTTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTc                             <  3:206489/149‑1 (MQ=255)
                                                                                                                aTTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTc                             >  3:123971/1‑149 (MQ=255)
                                                                                                                            gCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAAc                 >  7:268320/1‑149 (MQ=255)
                                                                                                                             cccGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACg                <  6:236411/149‑1 (MQ=255)
                                                                                                                              ccGCTCCCACAGGTTGTGGGTGAGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATACACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACgg               >  3:236853/1‑149 (MQ=255)
                                                                                                                               cGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGgt              <  2:21511/149‑1 (MQ=255)
                                                                                                                                    ccACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTc                                      >  3:104144/1‑120 (MQ=255)
                                                                                                                                    ccACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTc                                      <  4:104144/120‑1 (MQ=255)
                                                                                                                                    ccACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTcgg         >  1:300692/1‑149 (MQ=255)
                                                                                                                                     cacaACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTcggc        >  3:284126/1‑149 (MQ=255)
                                                                                                                                        aaCCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTcggcgg      >  3:222822/1‑148 (MQ=255)
                                                                                                                                         aCCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTCGGCGGCa    <  7:1888/149‑1 (MQ=255)
                                                                                                                                           ttgtgGGTGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTCGGCGGCAgg  >  2:106980/2‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
CTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTCGGCGGCAGG  >  NC_002947/5790013‑5790300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑