Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,797,312 G→T 5.3% R196R (CGC→CGA)  gltB ← L‑glutamate synthase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,797,3120GT 5.3% 250.1 / 3.7 76R196R (CGC→CGA) gltBL‑glutamate synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (38/34);  new base T (2/2);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

ATCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAGGCCTTCACC  >  NC_002947/5797169‑5797458
                                                                                                                                                |                                                                                                                                                   
aTCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCgg                                                                                                                                                >  5:7676/1‑149 (MQ=255)
     gCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGCGATCTCGCGGcgt                                                                                                                                             <  2:4979/147‑3 (MQ=255)
        gTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCAtcatca                                                                                                                                        <  6:134181/149‑1 (MQ=255)
              ggCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGCGATCTCGCGGcgtt                                                                                                                                            >  4:161032/1‑136 (MQ=255)
              ggCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGCGATCTCGCGGcgtt                                                                                                                                            <  3:161032/139‑4 (MQ=255)
               gCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGGAGTGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCttt                                                                                                                                 <  4:100002/149‑1 (MQ=255)
                cGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTg                                                                                                                                <  3:99281/149‑1 (MQ=255)
                    gAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTg                                                                                                                                >  3:106136/1‑145 (MQ=255)
                         ggAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGatgatg                                                                                                                       <  6:7676/149‑1 (MQ=255)
                          gAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCAtcatca                                                                                                                                        <  5:233602/131‑1 (MQ=255)
                          gAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCAtcatca                                                                                                                                        >  6:233602/1‑131 (MQ=255)
                          gAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATgg                                                                                                                      >  6:127663/1‑149 (MQ=255)
                                cTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTTGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTtgt                                                                                                                >  6:300947/1‑149 (MQ=255)
                                 tGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTtgtg                                                                                                               >  4:168756/1‑149 (MQ=255)
                                           gCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTg                                                                                                     >  8:159095/1‑149 (MQ=255)
                                            ccATTTCGGCAGGGGGGTGGTGGAGCAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGc                                                                                                    <  8:219481/149‑1 (MQ=255)
                                              aTTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTg                                                                                                     >  6:106105/1‑146 (MQ=255)
                                                 tCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCa                                                                                                   >  6:29323/1‑145 (MQ=255)
                                                 tCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCa                                                                                                   <  5:29323/145‑1 (MQ=255)
                                                  cGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATg                                                                                               <  2:147972/148‑1 (MQ=255)
                                                    gCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAg                                                                                            >  5:274089/1‑149 (MQ=255)
                                                    gCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAg                                                                                            <  4:17261/149‑1 (MQ=255)
                                                     cAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTa                                                                                             <  3:56436/147‑1 (MQ=255)
                                                     cAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTa                                                                                             >  4:56436/1‑147 (MQ=255)
                                                      aGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTg                                                                                          <  8:25568/149‑1 (MQ=255)
                                                        ggTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGt                                                                                        >  7:221010/1‑149 (MQ=255)
                                                               gtggAGAAGCGCTTGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCgaga                                                                                                          <  1:152741/124‑1 (MQ=255)
                                                               gtggAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCgaga                                                                                                          >  2:152741/1‑124 (MQ=255)
                                                                 ggAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGcgtcgt                                                                               >  1:113078/1‑149 (MQ=255)
                                                                  gagaAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTtgt                                                                                                                <  7:87799/115‑1 (MQ=255)
                                                                  gagaAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTtgt                                                                                                                >  8:87799/1‑115 (MQ=255)
                                                                     aaGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTgg                                                                             >  6:229077/1‑147 (MQ=255)
                                                                                    acGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAgatg                                                                                                                          >  2:104672/1‑87 (MQ=255)
                                                                                    acGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACcgacga                                                            <  7:159095/149‑1 (MQ=255)
                                                                                    acGCAGATCCCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAgatg                                                                                                                          <  1:104672/87‑1 (MQ=255)
                                                                                     cGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGacgaa                                                           <  6:274089/149‑1 (MQ=255)
                                                                                     cGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGacgaa                                                           <  3:168756/149‑1 (MQ=255)
                                                                                      gCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTg                                                                              >  6:220576/1‑129 (MQ=255)
                                                                                      gCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTg                                                                              <  5:220576/129‑1 (MQ=255)
                                                                                      gCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGacgaac                                                          >  4:141501/1‑149 (MQ=255)
                                                                                      gCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGacgaac                                                          >  6:318816/1‑149 (MQ=255)
                                                                                      gCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGacgaac                                                          >  6:180784/1‑149 (MQ=255)
                                                                                         gATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAacgac                                                       <  2:113078/149‑1 (MQ=255)
                                                                                            cgcgGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAacgacgac                                                    >  8:70365/1‑149 (MQ=255)
                                                                                             gcgGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAacgacgacg                                                   <  8:221010/149‑1 (MQ=255)
                                                                                                    gCAGGCGCTCGTCACCCAGGTCTGGATAAAACGGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑Ct                                            >  1:211315/1‑149 (MQ=255)
                                                                                                    gCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑Ct                                            <  5:106105/148‑1 (MQ=255)
                                                                                                    gCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTATAGATGATGGTCTTGTGCGAGAAGCTGc                                                                                                    <  2:101056/93‑1 (MQ=255)
                                                                                                    gCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTATAGATGATGGTCTTGTGCGAGAAGCTGc                                                                                                    >  1:101056/1‑93 (MQ=255)
                                                                                                      aGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGAt                                                                                                <  3:310024/95‑1 (MQ=255)
                                                                                                      aGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGAt                                                                                                >  4:310024/1‑95 (MQ=255)
                                                                                                       ggCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAAc                                        <  5:180784/149‑1 (MQ=255)
                                                                                                        gcgcTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGaa                                         >  8:193945/1‑147 (MQ=255)
                                                                                                        gcgcTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGaa                                         >  7:196730/1‑147 (MQ=255)
                                                                                                                 aCCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATg                               <  2:211315/148‑1 (MQ=255)
                                                                                                                  cccAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGc                             <  5:127663/149‑1 (MQ=255)
                                                                                                                   ccAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGAt                                >  2:138920/1‑145 (MQ=255)
                                                                                                                   ccAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGAt                                <  1:138920/145‑1 (MQ=255)
                                                                                                                     aGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGaa                          >  6:112421/1‑149 (MQ=255)
                                                                                                                      ggTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAAt                         <  7:193945/149‑1 (MQ=255)
                                                                                                                       gTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGcgtcgt                                                                               <  4:1268/95‑1 (MQ=255)
                                                                                                                        tCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTa                                                                                                                              <  6:206966/47‑1 (MQ=255)
                                                                                                                        tCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTa                                                                                                                              >  5:206966/1‑47 (MQ=255)
                                                                                                                        tCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑Ct                                            >  4:201811/1‑128 (MQ=255)
                                                                                                                        tCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑Ct                                            <  3:201811/128‑1 (MQ=255)
                                                                                                                           ggATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTg                    >  7:267810/1‑149 (MQ=255)
                                                                                                                            gATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTgg                   >  1:30659/1‑149 (MQ=255)
                                                                                                                            gATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTgg                   >  4:285043/1‑149 (MQ=255)
                                                                                                                            gATAAAAC‑GCCGCGAGATCGCGCGGCATAATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTgg                   >  1:19787/1‑149 (MQ=255)
                                                                                                                              tgatgatgccgcGCGATCTCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTGGt                  >  2:83581/8‑149 (MQ=255)
                                                                                                                               aaaaC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTGGTCg                >  2:318012/1‑149 (MQ=255)
                                                                                                                                 aaC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTGGTCGCt              >  8:290938/1‑149 (MQ=255)
                                                                                                                                  aC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACCTGAACAGCTTGATGGCGAATTCCTgg                   <  1:171067/144‑1 (MQ=255)
                                                                                                                                  aC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTgg                   >  2:171067/1‑143 (MQ=255)
                                                                                                                                        gcgAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAGGcc        <  3:141501/149‑1 (MQ=255)
                                                                                                                                              tCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAGGCCTTCAcc  <  2:30659/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ATCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAAC‑GCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCA‑CTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAGGCCTTCACC  >  NC_002947/5797169‑5797458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑