Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,851,171 T→A 11.8% intergenic (+34/+34) ilvD → / ← PP_5129 dihydroxy‑acid dehydratase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,851,1710TA11.8% 135.1 / 8.6 51intergenic (+34/+34)ilvD/PP_5129dihydroxy‑acid dehydratase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/25);  new base A (3/3);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

GCCAGCCGAGGTCCGTCCACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCGGCACCTACCCC  >  NC_002947/5851023‑5851315
                                                                                                                                                    |                                                                                                                                                
gccagccGAGGTCCGTCCACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCt                                                                                                                                                  >  4:45480/1‑149 (MQ=255)
             cgtccACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCAGGGCGCCGGttct                                                                                                                                     <  4:215677/149‑3 (MQ=255)
               tccACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCTGtttttt                                                                                                                                   >  4:104634/1‑149 (MQ=255)
               tccACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGtttttt                                                                                                                                   <  6:291703/149‑1 (MQ=255)
                ccACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCg                                                                                                                                          >  5:52181/1‑141 (MQ=255)
                ccACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCg                                                                                                                                          <  6:52181/141‑1 (MQ=255)
                   cGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGa                                                                                                                               <  3:45480/149‑1 (MQ=255)
                     cAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCAGGGCGCCGGttcttatttcac                                                                                                                             <  2:243275/149‑5 (MQ=255)
                           aaCCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCAt                                                                                                                       >  2:294166/1‑149 (MQ=255)
                               accGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGAc                                                                                                                   <  1:294166/149‑1 (MQ=255)
                                    gcTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCa                                                                                                              <  6:292775/149‑1 (MQ=255)
                                    gcTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCa                                                                                                              <  3:202155/149‑1 (MQ=255)
                                      tCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTc                                                                                                                         >  3:274389/1‑136 (MQ=255)
                                      tCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTc                                                                                                                         <  4:274389/136‑1 (MQ=255)
                                      tCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGt                                                                                                            >  7:147030/1‑149 (MQ=255)
                                       cAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCAGGGCGCCGGttctta                                                                                                                                   <  7:139475/125‑2 (MQ=255)
                                       cAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCAGGGCGCCGGttctta                                                                                                                                   >  8:139475/1‑124 (MQ=255)
                                        aaGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGt                                                                                                            <  7:92675/147‑1 (MQ=255)
                                           gCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGc                                                                                                       >  6:211554/1‑149 (MQ=255)
                                           gCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGc                                                                                                       <  8:147030/149‑1 (MQ=255)
                                             cTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGc                                                                                                       >  8:249937/1‑147 (MQ=255)
                                                tGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAg                                                                                                    <  1:242418/147‑1 (MQ=255)
                                                        tgGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGt                                                                                          >  1:201992/1‑149 (MQ=255)
                                                          gCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGt                                                                                          <  3:104634/147‑1 (MQ=255)
                                                          gCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCg                                                                                        >  4:211816/1‑149 (MQ=255)
                                                            caccaGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGa                                                                                      <  3:211816/149‑1 (MQ=255)
                                                                 gTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGTAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGaa                                                                                 <  6:283985/149‑1 (MQ=255)
                                                                 gTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGaa                                                                                 <  7:249937/149‑1 (MQ=255)
                                                                  tGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGaa                                                                                 <  8:67783/148‑1 (MQ=255)
                                                                  tGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGaa                                                                                 >  7:67783/1‑148 (MQ=255)
                                                                      gACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAg                                                                                                        >  8:45286/1‑121 (MQ=255)
                                                                      gACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAg                                                                                                        <  7:45286/121‑1 (MQ=255)
                                                                                     cgTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGgaga                                                             <  5:211554/149‑1 (MQ=255)
                                                                                          cAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGAt                                                        >  7:248019/1‑149 (MQ=255)
                                                                                           aaGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCTCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGAtt                                                       <  2:83844/149‑1 (MQ=255)
                                                                                           aaGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGAtt                                                       >  1:298651/1‑149 (MQ=255)
                                                                                             ggCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGTTCGAAACATTGACAGCTTTTGGAGAGCGATTc                                                      >  1:159976/1‑148 (MQ=255)
                                                                                             ggCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCGGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTcc                                                     <  5:262112/149‑1 (MQ=255)
                                                                                                    cTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACAt                                                                             >  4:180439/1‑118 (MQ=255)
                                                                                                    cTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACAt                                                                             <  3:180439/118‑1 (MQ=255)
                                                                                                             ctctGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATc                                      >  8:47477/1‑148 (MQ=255)
                                                                                                                      gCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTg                            >  1:44497/1‑149 (MQ=255)
                                                                                                                             gCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCttttt                      <  2:44497/148‑1 (MQ=255)
                                                                                                                             gCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTc                     <  8:248019/149‑1 (MQ=255)
                                                                                                                                tGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTg                                                                 <  4:74990/102‑1 (MQ=255)
                                                                                                                                tGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTg                                                                 >  3:74990/1‑102 (MQ=255)
                                                                                                                                     aaaaaaCCGGCGCCCAGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCg             >  2:29342/5‑149 (MQ=255)
                                                                                                                                      aaGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCgg            >  8:192560/1‑149 (MQ=255)
                                                                                                                                        aaaCCGGCGCCCAGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCGGCa          >  7:280884/2‑149 (MQ=255)
                                                                                                                                            cGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGTCGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCAt                                       <  2:239277/116‑1 (MQ=255)
                                                                                                                                            cGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCAt                                       >  1:239277/1‑116 (MQ=255)
                                                                                                                                                gcCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGTGGCACCTAcccc  <  2:201992/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCCAGCCGAGGTCCGTCCACGCAAGGTAACCACCGCGCTCAAGGCCTATGCCCTGCTGGCCACCAGTGCCGACAAGGGTGCGGTGCGTAACAAGGCAATGCTGGAAGGCCTCTGAGGTGCTTTTAGCGTGAAATAAGAACCGGCGCCCTGGGCGCCGGTTTTTTTTGATTGTTCATTGACGGTCAGTGAAGCGAGGTGGCTGGGTCGGATCGAAACATTGACAGCTTTTGGAGAGCGATTCCAAGTGCCTCGTCATCGAACAGTTTGCTTTTTCGAGCAGCGGCACCTACCCC  >  NC_002947/5851023‑5851315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑