Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,883,595	C→G	7.6%	A60G (GCC→GGC)	PP_5157 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,883,595	0	C	G	7.6%	233.0 / 4.7	73	A60G (GCC→GGC)	PP_5157	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (37/30);  new base G (3/3);  total (40/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGC  >  NC_002947/5883450‑5883740                                                                                                                                                  |                                                                                                                                                  cTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCt                                                                                                                                                <  3:285887/149‑1 (MQ=255)    aTCTGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGGGAACTGCGCATTGCCGTACAGGGCGAGAACTCGGCATACGGCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTg                                                                                                                                               <  5:314568/147‑1 (MQ=255)    aTCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTgg                                                                                                                                              <  8:54160/148‑1 (MQ=255)           cATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGaa                                                                                                                                      >  3:199621/1‑149 (MQ=255)            aTTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAAc                                                                                                                                     <  7:13997/149‑1 (MQ=255)                 cTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTg                                                                                                                                   <  6:13923/146‑1 (MQ=255)                 cTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTg                                                                                                                                   >  5:13923/1‑146 (MQ=255)                              gATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCAAAGGAACTGGATTTGCGCGCCGAGt                                                                                                                   >  3:108348/1‑149 (MQ=255)                              gATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGACGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGt                                                                                                                   >  1:72814/1‑149 (MQ=255)                               aTCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCA‑GCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGt                                                                                                                   <  4:20903/147‑1 (MQ=255)                                  gacgaAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTtcgt                                                                                                               >  1:185017/1‑149 (MQ=255)                                     gaAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGa                                                                                                            <  4:199621/149‑1 (MQ=255)                                        aTCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGc                                                                                                         >  8:177595/1‑149 (MQ=255)                                          cGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGccc                                                                                                       >  6:249683/1‑149 (MQ=255)                                            ccGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCg                                                                                                     >  3:303920/1‑149 (MQ=255)                                            ccGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCg                                                                                                     >  2:191215/1‑149 (MQ=255)                                                ccGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTa                                                                                                 <  7:282948/149‑1 (MQ=255)                                                ccGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTa                                                                                                 >  5:49542/1‑149 (MQ=255)                                                 cGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTAc                                                                                                <  5:249683/149‑1 (MQ=255)                                                 cGGGGCCAACTGCCCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGATTCGACATCGAATTTGGCCAGGCCCACGACAAGGAACTCGAATTGCGCGCCGAGATCGTCGAATCCCCGCCTAc                                                                                                >  4:153913/1‑149 (MQ=255)                                                   gggCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTAcc                                                                                               >  7:40977/1‑148 (MQ=255)                                                   gggCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTAcc                                                                                               >  3:290040/1‑148 (MQ=255)                                                     gCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCg                                                                                              >  6:25457/1‑147 (MQ=255)                                                      cGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAg                                                                                           >  7:263316/1‑149 (MQ=255)                                                               cATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCgg                                                                                  <  8:263316/149‑1 (MQ=255)                                                               cATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCgg                                                                                  <  7:177595/149‑1 (MQ=255)                                                                aTTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCggg                                                                                 >  1:324932/1‑149 (MQ=255)                                                                    ccGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGgtgt                                                                              <  5:25457/148‑1 (MQ=255)                                                                      gTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCga                                                                           <  4:303920/149‑1 (MQ=255)                                                                          aGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCCCCTCACAGGTTTCGACATCGAATTTGGCCCGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTGGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGt                                                                       >  6:44879/1‑149 (MQ=255)                                                                             ccGAGAACTCGCCATACGCCTTCAAGCAATACTACCTCCTGACAGGTTTCGACATCGAATTTGGCCAGGGCCTGGGCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGTCTACCGAAGTGCTGGCTGGTGTCGAGAGTGGc                                                                    <  3:153913/149‑1 (MQ=255)                                                                               gagaACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCaa                                                                  <  6:49542/149‑1 (MQ=255)                                                                               gagaACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCaa                                                                  >  3:256327/1‑149 (MQ=255)                                                                                agaACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAg                                                                 >  6:95532/1‑149 (MQ=255)                                                                                   aCTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCTGGTGTCGAGAGTGGCAAGTAc                                                              <  1:41330/149‑1 (MQ=255)                                                                                     tCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTtc                                                                                                                 >  4:168369/1‑96 (MQ=255)                                                                                       gCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACa                                                          >  3:155685/1‑149 (MQ=255)                                                                                        ccATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACAt                                                         <  2:72814/149‑1 (MQ=255)                                                                                        ccATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCAAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACAt                                                         >  7:281718/1‑149 (MQ=255)                                                                                         cATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATc                                                        <  2:185017/149‑1 (MQ=255)                                                                                              gCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCAtt                                                   >  1:175643/1‑149 (MQ=255)                                                                                                cTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCa                                                 >  4:320461/1‑149 (MQ=255)                                                                                                   caagcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCAcc                                               <  3:320461/148‑1 (MQ=255)                                                                                                   caagcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCAc                                                <  4:155685/147‑1 (MQ=255)                                                                                                      gcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGt                                           >  6:99887/1‑149 (MQ=255)                                                                                                       caagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTc                                          <  2:175643/149‑1 (MQ=255)                                                                                                          gacgacCACCTGACAGGTTTCGTCATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAg                                       <  4:290040/149‑1 (MQ=255)                                                                                                           acgacCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGt                                      <  5:95532/149‑1 (MQ=255)                                                                                                                  ccTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGt                                      >  4:273188/1‑142 (MQ=255)                                                                                                                  ccTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGt                                      <  3:273188/142‑1 (MQ=255)                                                                                                                  ccTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCAAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGc                               <  8:281718/149‑1 (MQ=255)                                                                                                                        aGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTTCCCAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACAACGCATTCACCCCGTCGAGTGAATCGCTCACAg                         >  8:253790/1‑149 (MQ=255)                                                                                                                        aGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCAcc                                               <  7:251725/127‑1 (MQ=255)                                                                                                                        aGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAg                         >  2:103184/1‑149 (MQ=255)                                                                                                                        aGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAg                         <  2:324932/149‑1 (MQ=255)                                                                                                                          gTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGc                       >  4:63551/1‑149 (MQ=255)                                                                                                                            ttCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGa                     >  5:56029/1‑149 (MQ=255)                                                                                                                               gACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGc                               <  8:199398/136‑1 (MQ=255)                                                                                                                               gACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGc                               >  7:199398/1‑136 (MQ=255)                                                                                                                                 cATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGGTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAg                         >  4:211115/1‑140 (MQ=255)                                                                                                                                 cATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTcac                           >  5:24581/1‑138 (MQ=255)                                                                                                                                 cATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTcac                           <  6:24581/138‑1 (MQ=255)                                                                                                                                 cATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAg                         <  3:211115/140‑1 (MQ=255)                                                                                                                                   tCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCg              >  6:35445/1‑149 (MQ=255)                                                                                                                                    gttccttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCg              >  2:297763/6‑148 (MQ=255)                                                                                                                                     gAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCt            >  2:236141/1‑149 (MQ=255)                                                                                                                                      aaTTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTc           <  1:191215/149‑1 (MQ=255)                                                                                                                                         ttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGAt        >  1:80118/1‑149 (MQ=255)                                                                                                                                          tGGCCAGGGCCTGGCCAAGGAACTGGTTTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTTGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGAt        <  4:108348/148‑1 (MQ=255)                                                                                                                                            gCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCAccc                                              >  4:24144/1‑108 (MQ=255)                                                                                                                                            gCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCAccc                                              <  3:24144/108‑1 (MQ=255)                                                                                                                                              cAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAg   >  4:243835/1‑149 (MQ=255)                                                                                                                                               aGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTCGCGCGTGTCGAGAGTGCCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCCATGTAAGc  >  7:238368/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGC  >  NC_002947/5883450‑5883740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑