Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,887,056 T→G 12.7% intergenic (‑41/‑89) PP_5161 ← / → PP_5162 lysophospholipase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,887,0560TG12.7% 145.5 / 14.2 55intergenic (‑41/‑89)PP_5161/PP_5162lysophospholipase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (26/22);  new base G (3/4);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

GCAGGCGGTCGGGGTGAAAGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGGCGC  >  NC_002947/5886910‑5887200
                                                                                                                                                  |                                                                                                                                                
gcAGGCGGTCGGGGTGAAAGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGTGGGAGCGGCCTTGcg                                                                                                                                                >  7:203202/1‑149 (MQ=255)
         cGGGGTGAAAGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAg                                                                                                                                       >  7:66388/1‑149 (MQ=255)
              tGAAAGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGc                                                                                                                                  >  2:105824/1‑149 (MQ=255)
                aaaGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTc                                                                                                                                >  7:56879/1‑149 (MQ=255)
                  aGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTcc                                                                                                                               >  6:24359/1‑148 (MQ=255)
                   ggCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCca                                                                                                                             <  2:253303/149‑1 (MQ=255)
                    gCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCcac                                                                                                                            <  5:116663/149‑1 (MQ=255)
                      aGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGTGGGAGCGGCCTTGCGTCGCGAAAGGCCGCTCCcgcaa                                                                                                                          <  8:203202/149‑2 (MQ=38)
                      aGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTGCGTCGCGAAAGGCCGCTCCcgcaa                                                                                                                          <  2:118272/149‑2 (MQ=39)
                      aGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCGCTTGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAg                                                                                                                          <  8:146885/149‑1 (MQ=255)
                             aTCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTGCGTCGCGAAAGGcc                                                                                                                                    >  1:65642/1‑132 (MQ=255)
                             aTCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTGCGTCGCGAAAGGcc                                                                                                                                    <  2:65642/132‑1 (MQ=255)
                                      aTCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCtt                                                                                                          <  3:141048/149‑1 (MQ=255)
                                      aTCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCtt                                                                                                          <  2:41983/149‑1 (MQ=255)
                                       tCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCttt                                                                                                         <  8:66388/149‑1 (MQ=255)
                                        ccACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCttt                                                                                                         >  1:152393/1‑148 (MQ=255)
                                         cacaTTGATGCAAGTATTGATCTGGTATCTTCAGCTCTGGCCGGCGCCATGTCAAGCTTGCTCACCTCGAACAACCAGCATAGAACTAATTGCGGGAGCGGCCTTTCGCGACGGAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTa                                                                                                       <  6:37708/149‑1 (MQ=255)
                                         cacaTTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCttt                                                                                                         >  6:227896/1‑147 (MQ=255)
                                            aTTGATGCTAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACAAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGCCGCCAGCGTTTAGa                                                                                                     >  2:320069/1‑148 (MQ=255)
                                                 tGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGa                                                                                               <  1:29784/149‑1 (MQ=255)
                                                   cAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAg                                                                                              <  1:105824/148‑1 (MQ=255)
                                                    aaGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGc                                                                                             >  2:98015/1‑148 (MQ=255)
                                                           gATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGt                                                                                     >  5:84348/1‑149 (MQ=255)
                                                            aTCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGAATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGtt                                                                                    >  7:299293/1‑149 (MQ=255)
                                                                ggAATCTTCAGCTCTGGCCGGTGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCtt                                                                                >  6:163209/1‑149 (MQ=255)
                                                                ggAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCtt                                                                                >  7:218593/1‑149 (MQ=255)
                                                                      ttCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGaaa                                                                                                   >  1:161702/1‑124 (MQ=255)
                                                                      ttCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGaaa                                                                                                   <  2:161702/124‑1 (MQ=255)
                                                                      ttCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCcac                                                                            >  3:172234/1‑147 (MQ=255)
                                                                         aGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACa                                                                                       >  5:114817/1‑133 (MQ=255)
                                                                         aGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACa                                                                                       <  6:114817/133‑1 (MQ=255)
                                                                           ctctGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGGATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGcc                                                                     >  1:68935/1‑149 (MQ=255)
                                                                                gCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCAc                                                                   <  8:56879/146‑1 (MQ=255)
                                                                                  cGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGAct                                                              >  6:80242/1‑149 (MQ=255)
                                                                                    gcgcCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGActct                                                            <  6:84348/149‑1 (MQ=255)
                                                                                       ccATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCgg                                                                                                                   <  7:134881/91‑1 (MQ=255)
                                                                                       ccATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCgg                                                                                                                   >  8:134881/1‑91 (MQ=255)
                                                                                        cATGGCAAGATTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCAt                                                        <  3:67445/149‑1 (MQ=255)
                                                                                          tGGCAAGCATGCTCACCTCGAACAACCAGAATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGACACCGACTCTTCATg                                                       >  4:52164/1‑148 (MQ=255)
                                                                                             cAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATa                                                   <  1:37374/149‑1 (MQ=255)
                                                                                                          cTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAAtcatca                                       >  8:23241/1‑148 (MQ=255)
                                                                                                          cTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAAtcatca                                       >  4:17306/1‑148 (MQ=255)
                                                                                                                  acCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCtgt                              <  5:227896/149‑1 (MQ=255)
                                                                                                                      gCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCAc                          >  4:143213/1‑149 (MQ=255)
                                                                                                                        aTCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACgg                        <  2:68935/149‑1 (MQ=255)
                                                                                                                                     cGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCttgt            <  3:17306/148‑1 (MQ=255)
                                                                                                                                     cGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCttgt            <  8:218593/148‑1 (MQ=255)
                                                                                                                                       ggAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGATATTGAGCAAGTCAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTgg         <  3:52164/149‑1 (MQ=255)
                                                                                                                                       ggAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTgg         >  6:77634/1‑149 (MQ=255)
                                                                                                                                          gCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCg      >  7:209656/1‑149 (MQ=255)
                                                                                                                                          gCGGCCTTGCGTCGCGAAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCttgtt           <  6:296192/144‑1 (MQ=38)
                                                                                                                                          gCGGCCTTGCGTCGCGAAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCttgtt           >  5:296192/1‑144 (MQ=38)
                                                                                                                                            ggCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGgc    <  1:98015/149‑1 (MQ=255)
                                                                                                                                            ggCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGgc    >  3:70677/1‑149 (MQ=255)
                                                                                                                                              ccTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGgcgc  >  1:176704/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCAGGCGGTCGGGGTGAAAGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGGCGC  >  NC_002947/5886910‑5887200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑