| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,887,061 | A→G | 10.3% | intergenic (‑46/‑84) | PP_5161 ← / → PP_5162 | lysophospholipase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,887,061 | 0 | A | G | 10.3% | 154.0 / 7.3 | 58 | intergenic (‑46/‑84) | PP_5161/PP_5162 | lysophospholipase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (28/24); new base G (2/4); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
CGGGGTGAAAGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGGCGCCGTAATCC > NC_002947/5886919‑5887208 | cGGGGTGAAAGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAg > 7:66388/1‑149 (MQ=255) tGAAAGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGc > 2:105824/1‑149 (MQ=255) aaaGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTc > 7:56879/1‑149 (MQ=255) aGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTcc > 6:24359/1‑148 (MQ=255) ggCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCca < 2:253303/149‑1 (MQ=255) gCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCcac < 5:116663/149‑1 (MQ=255) aGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGTGGGAGCGGCCTTGCGTCGCGAAAGGCCGCTCCcgcaa < 8:203202/149‑2 (MQ=38) aGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTGCGTCGCGAAAGGCCGCTCCcgcaa < 2:118272/149‑2 (MQ=39) aGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCGCTTGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAg < 8:146885/149‑1 (MQ=255) aTCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTGCGTCGCGAAAGGcc > 1:65642/1‑132 (MQ=255) aTCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTGCGTCGCGAAAGGcc < 2:65642/132‑1 (MQ=255) aTCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCtt < 3:141048/149‑1 (MQ=255) aTCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCtt < 2:41983/149‑1 (MQ=255) tCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCttt < 8:66388/149‑1 (MQ=255) ccACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCttt > 1:152393/1‑148 (MQ=255) cacaTTGATGCAAGTATTGATCTGGTATCTTCAGCTCTGGCCGGCGCCATGTCAAGCTTGCTCACCTCGAACAACCAGCATAGAACTAATTGCGGGAGCGGCCTTTCGCGACGGAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTa < 6:37708/149‑1 (MQ=255) cacaTTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCttt > 6:227896/1‑147 (MQ=255) aTTGATGCTAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACAAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGCCGCCAGCGTTTAGa > 2:320069/1‑148 (MQ=255) tGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGa < 1:29784/149‑1 (MQ=255) cAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAg < 1:105824/148‑1 (MQ=255) aaGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGc > 2:98015/1‑148 (MQ=255) gATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGt > 5:84348/1‑149 (MQ=255) aTCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGAATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGtt > 7:299293/1‑149 (MQ=255) ggAATCTTCAGCTCTGGCCGGTGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCtt > 6:163209/1‑149 (MQ=255) ggAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCtt > 7:218593/1‑149 (MQ=255) ttCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGaaa < 2:161702/124‑1 (MQ=255) ttCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGaaa > 1:161702/1‑124 (MQ=255) ttCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCcac > 3:172234/1‑147 (MQ=255) aGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACa > 5:114817/1‑133 (MQ=255) aGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACa < 6:114817/133‑1 (MQ=255) ctctGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGGATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGcc > 1:68935/1‑149 (MQ=255) gCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCAc < 8:56879/146‑1 (MQ=255) cGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGAct > 6:80242/1‑149 (MQ=255) gcgcCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGActct < 6:84348/149‑1 (MQ=255) ccATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCgg > 8:134881/1‑91 (MQ=255) ccATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCgg < 7:134881/91‑1 (MQ=255) cATGGCAAGATTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCAt < 3:67445/149‑1 (MQ=255) tGGCAAGCATGCTCACCTCGAACAACCAGAATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGACACCGACTCTTCATg > 4:52164/1‑148 (MQ=255) cAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATa < 1:37374/149‑1 (MQ=255) cTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAAtcatca > 4:17306/1‑148 (MQ=255) cTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAAtcatca > 8:23241/1‑148 (MQ=255) acCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCtgt < 5:227896/149‑1 (MQ=255) gCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCAc > 4:143213/1‑149 (MQ=255) aTCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACgg < 2:68935/149‑1 (MQ=255) cGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCttgt < 8:218593/148‑1 (MQ=255) cGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCttgt < 3:17306/148‑1 (MQ=255) ggAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGATATTGAGCAAGTCAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTgg < 3:52164/149‑1 (MQ=255) ggAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTgg > 6:77634/1‑149 (MQ=255) gCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCg > 7:209656/1‑149 (MQ=255) gCGGCCTTGCGTCGCGAAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCttgtt < 6:296192/144‑1 (MQ=38) gCGGCCTTGCGTCGCGAAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCttgtt > 5:296192/1‑144 (MQ=38) ggCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGgc < 1:98015/149‑1 (MQ=255) ggCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGgc > 3:70677/1‑149 (MQ=255) ccTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGgcgc > 1:176704/1‑149 (MQ=255) gcgACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGGCGCCGTaa < 5:163209/148‑1 (MQ=255) cgACGCAAGGCCGCTCCCACAGGAATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGGCGCCGTAATc < 8:299293/149‑1 (MQ=255) gACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGGCGCCGTAATcc > 7:27632/1‑149 (MQ=255) gACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGGCGCCGTAATcc > 5:9078/1‑149 (MQ=255) | CGGGGTGAAAGGCAGGGGGCATCGGGTACATCCACATTGATGCAAGTATTGATCTGGAATCTTCAGCTCTGGCCGGCGCCATGGCAAGCTTGCTCACCTCGAACAACCAGCATCGAACTAATTGCGGGAGCGGCCTTTCGCGACGCAAGGCCGCTCCCACAGGGATCGGCGCCAGCTTTTAGAAATTGAGCAAGACAGTTGCTTCCACAAGGGCCACCGACTCTTCATGAATAGCCGAATCATCAAACCGCTGTGCACGGTCATCGCCTTGTTGGTCGGCGCCGTAATCC > NC_002947/5886919‑5887208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |