Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,916,535 C→T 7.8% D98N (GAT→AAT)  argE ← acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,916,5350CT 7.8% 148.4 / 2.3 51D98N (GAT→AAT) argEacetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base C (23/24);  new base T (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTGCCGGGGCTGACCTGCTGGATAT  >  NC_002947/5916391‑5916681
                                                                                                                                                |                                                                                                                                                   
cGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGtc                                                                                                                                                 <  6:96281/149‑1 (MQ=255)
  gACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGtct‑c                                                                                                                                              <  8:41028/149‑1 (MQ=255)
      tGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATc                                                                                                                                          <  5:114335/149‑1 (MQ=255)
         aGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGc                                                                                                                                       <  3:39915/149‑1 (MQ=255)
              tcttcGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAg                                                                                                                                  >  2:20520/1‑149 (MQ=255)
               cttcGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGc                                                                                                                                 >  2:296911/1‑149 (MQ=255)
                ttcGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATTGGTCT‑CGATCAGCTTCAGCg                                                                                                                                >  4:266381/1‑149 (MQ=255)
                ttcGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATTGGTCT‑CGATCAGAttgcgcg                                                                                                                                >  4:265301/1‑144 (MQ=255)
                  cgtcgCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGc                                                                                                                                 <  1:204045/146‑1 (MQ=255)
                  cgtcgCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCgg                                                                                                                               <  1:275877/148‑1 (MQ=255)
                   gtcgCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGt                                                                                                                             >  6:263909/1‑149 (MQ=255)
                                    tCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAATGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGt                                                                                                            >  7:84540/1‑149 (MQ=255)
                                      agcagcGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTc                                                                                                          <  4:177552/149‑1 (MQ=255)
                                               tCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAggg                                                                                                 <  5:121778/149‑1 (MQ=255)
                                                 cTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAgag                                                                                                 >  6:121778/1‑145 (MQ=255)
                                                          gTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCg                                                                                       <  1:20520/148‑1 (MQ=255)
                                                            gCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCtg                                                                                    >  4:108380/1‑149 (MQ=255)
                                                            gCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGGCT‑CGATCACCTTCAGCGGGTCGCTGGCCCCCAGCGGTTCGTCATAGGGCACGGTATCGCtg                                                                                    >  3:14234/1‑149 (MQ=255)
                                                             cTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCtgt                                                                                   <  3:108380/149‑1 (MQ=255)
                                                             cTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCtgt                                                                                   <  5:263909/149‑1 (MQ=255)
                                                              tCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCtgtg                                                                                  >  6:41807/1‑149 (MQ=255)
                                                               ccAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGAt                                                                                                                                           >  3:175551/1‑91 (MQ=255)
                                                               ccAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGAt                                                                                                                                           <  4:175551/91‑1 (MQ=255)
                                                                cagcagCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCt                                                                                                              <  5:213324/119‑1 (MQ=255)
                                                                cagcagCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCt                                                                                                              >  6:213324/1‑119 (MQ=255)
                                                                cagcagCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGcc                                                                                >  3:123218/1‑149 (MQ=255)
                                                                           tGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCAcc                                                                     <  4:123218/149‑1 (MQ=255)
                                                                              ccGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATTGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGc                                                                  <  3:265301/149‑1 (MQ=255)
                                                                              ccGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATTGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGc                                                                  <  3:266381/149‑1 (MQ=255)
                                                                              ccGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGc                                                                  >  5:31125/1‑149 (MQ=255)
                                                                                     gATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCg                                                           >  6:234464/1‑149 (MQ=255)
                                                                                           cAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCgg                                                     >  7:135685/1‑149 (MQ=255)
                                                                                                 gaagaaGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCAc                                               >  1:106421/1‑149 (MQ=255)
                                                                                                          cTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGc                                        >  1:103846/1‑147 (MQ=255)
                                                                                                                  cGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCTGCGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGc                                                                            >  1:55188/1‑104 (MQ=255)
                                                                                                                  cGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGc                                                                            <  2:55188/103‑1 (MQ=255)
                                                                                                                   gCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTg                              >  5:24583/1‑148 (MQ=255)
                                                                                                                    cAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGaa                            <  6:31125/149‑1 (MQ=255)
                                                                                                                    cAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGaa                            <  5:41807/149‑1 (MQ=255)
                                                                                                                    cAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGa                             >  7:285176/1‑148 (MQ=255)
                                                                                                                        tGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTg                        >  8:58234/1‑149 (MQ=255)
                                                                                                                          cccAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTGcc                      <  2:103846/149‑1 (MQ=255)
                                                                                                                           ccAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGt                                                    <  6:205200/118‑1 (MQ=255)
                                                                                                                               gCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTGCCgggtc                 >  6:324425/1‑147 (MQ=255)
                                                                                                                               gCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTGCCGGGGc                 >  7:103284/1‑149 (MQ=255)
                                                                                                                                  gACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTGCCGGGGCTGa              <  8:103284/149‑1 (MQ=255)
                                                                                                                                   aCCCAATGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTGCCGGGGCTGAc             <  8:84540/149‑1 (MQ=255)
                                                                                                                                   aCCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTGCCGGGGCTGAc             <  7:58234/149‑1 (MQ=255)
                                                                                                                                        tcGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTGCCGGGGCTGACctgct        <  1:319217/148‑1 (MQ=255)
                                                                                                                                         cGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTGCCGGGGCTGACctgctg       <  2:106421/149‑1 (MQ=255)
                                                                                                                                              aTCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTGCCGGGGCTGACCTGCTGGatat  >  1:25736/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CGGACATGGAGCTTTCTTCGTCGCAGGTGGCAAGGATCAGCAGCGGCTCCTTGAAGTCGTGCTCCAGCAGCGGGATGACCGCTTCGATGACCAGGGCGAAGAAGCCCTTCATGTCGCAGCTGCCCAGGCCGACCCAACGGCCATCGGTCT‑CGATCAGCTTCAGCGGGTCGCTGGCCCACAGCTGTTCGTCATAGGGCACGGTATCGCTGTGCCCGGCCAGCACCAGCCCTCCCGGGCCGGTGCCACGGCTGGCCAGCAGGTTGAACTTGCCGGGGCTGACCTGCTGGATAT  >  NC_002947/5916391‑5916681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑