Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,917,643	A→T	7.1%	Q234L (CAG→CTG)	ygiF →	thiamine triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,917,643	0	A	T	7.1%	159.5 / 3.1	56	Q234L (CAG→CTG)	ygiF	thiamine triphosphatase
Reads supporting (aligned to +/- strand):  ref base A (24/28);  new base T (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

CAGCCTGCCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGAC  >  NC_002947/5917497‑5917791                                                                                                                                                   |                                                                                                                                                       cAGCCTGCCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAgc                                                                                                                                                      <  8:32081/149‑1 (MQ=255)  aGCCTGCCATTGATGCCTTTCGACATCAGGAAGGCTGAGCGCGTTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAgct                                                                                                                                                     <  8:77355/149‑1 (MQ=255)   gCCTGCCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAgctg                                                                                                                                                    <  5:108567/149‑1 (MQ=255)     cTGCCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAgctgct                                                                                                                                                  >  2:6265/1‑149 (MQ=255)          aCTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGCCGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTggg                                                                                                                                               >  4:97258/1‑147 (MQ=255)          aCTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGgca                                                                                                                                             >  3:13721/1‑149 (MQ=255)               tGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCagcagc                                                                                                                                        <  2:84244/149‑1 (MQ=255)                   gTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCTGCTGCCCAGCAGctgccagg                                                                                                                                    <  7:61470/149‑2 (MQ=255)                   gTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGcagccagc                                                                                                                                    >  8:211123/1‑149 (MQ=255)                           tCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCAGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGcc                                                                                                                            >  8:261104/1‑149 (MQ=255)                                    cTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTAc                                                                                                                   <  1:47131/149‑1 (MQ=255)                                      gAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCg                                                                                                                 <  3:127070/149‑1 (MQ=255)                                             gTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTgg                                                                                                                                                <  4:253090/111‑1 (MQ=255)                                             gTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTgg                                                                                                                                                >  3:253090/1‑111 (MQ=255)                                                  cGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCa                                                                                                     >  8:279421/1‑149 (MQ=255)                                                  cGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCa                                                                                                     <  1:21676/149‑1 (MQ=255)                                                   gCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCAc                                                                                                    >  1:245494/1‑149 (MQ=255)                                                              ccGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCt                                                                                       >  4:9119/1‑149 (MQ=255)                                                               cgtaCAGCTACGAGCTTGACCTGCCGCTCTCCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGGCAGCGCCTGGGCGATCAGTACCGCCACAACGGCCTCTGG‑‑CGCCTGCTc                                                                                      <  2:229832/146‑1 (MQ=255)                                                               cGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTc                                                                                      <  7:261104/149‑1 (MQ=255)                                                                  aCAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCCGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAg                                                                                   <  1:6265/149‑1 (MQ=255)                                                                      cTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCTCGACTGCTCCAGGa                                                                                 >  1:114442/1‑149 (MQ=255)                                                                       tACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTg                                                                              >  6:164411/1‑149 (MQ=255)                                                                       tACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTg                                                                              >  6:106440/1‑149 (MQ=255)                                                                         cGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGTCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTg                                                                              <  7:211123/147‑1 (MQ=255)                                                                         cGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTggg                                                                            <  2:54002/149‑1 (MQ=255)                                                                            gCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCAGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTc                                                                          >  8:101118/1‑148 (MQ=255)                                                                             cTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTc                                                                          >  5:251872/1‑147 (MQ=255)                                                                             cTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGa                                                                        <  8:74290/149‑1 (MQ=255)                                                                                gACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAg                                                                       <  2:245494/147‑1 (MQ=255)                                                                                 aCCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGc                                                                    >  1:66990/1‑149 (MQ=255)                                                                                  ccTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGa                                                                        <  6:215172/144‑1 (MQ=255)                                                                                  ccTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGa                                                                        >  5:215172/1‑144 (MQ=255)                                                                                  ccTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGcc                                                                   <  5:106440/149‑1 (MQ=255)                                                                                      ccGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTg                                                                 <  7:303678/147‑1 (MQ=255)                                                                                      ccGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTg                                                                 >  8:303678/1‑147 (MQ=255)                                                                                         cacaCCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTgagcga                                                            <  2:66990/149‑1 (MQ=255)                                                                                          acacCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTgagccag                                                           >  8:217884/1‑149 (MQ=255)                                                                                            acCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCt                                                         >  5:224958/1‑149 (MQ=255)                                                                                            acCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCt                                                         >  7:13144/1‑149 (MQ=255)                                                                                                       cGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCa                                              >  4:173810/1‑149 (MQ=255)                                                                                                        gCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGCGCGCAGCTCGCTCag                                             <  3:13717/149‑2 (MQ=255)                                                                                                          cGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCg                                           <  3:9119/149‑1 (MQ=255)                                                                                                                  cccTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTg                                   <  7:101118/149‑1 (MQ=255)                                                                                                                   ccTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTgg                                  >  1:16832/1‑149 (MQ=255)                                                                                                                    cTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTggg                                 <  5:164411/149‑1 (MQ=255)                                                                                                                    cTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTggg                                 >  8:280867/1‑149 (MQ=255)                                                                                                                    cTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTggg                                 <  7:280867/149‑1 (MQ=255)                                                                                                                          gacgCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGgc                           <  6:251872/149‑1 (MQ=255)                                                                                                                            cgCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCGCTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGgcag                         <  4:13721/149‑1 (MQ=255)                                                                                                                            cgCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGgcag                         <  6:224958/149‑1 (MQ=255)                                                                                                                                              cTGGCTGCTGCCCAGCAGCTGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCAccc       >  5:302000/1‑149 (MQ=255)                                                                                                                                              cTGGCTGCTGCCCAGCAGCTGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCAccc       <  6:302000/149‑1 (MQ=255)                                                                                                                                              cTGGCTGCTGCCCAGCAGCTGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCAccc       >  6:320170/1‑149 (MQ=255)                                                                                                                                                 gCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCcgcg    <  8:13144/149‑1 (MQ=255)                                                                                                                                                 gCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCcgcg    >  8:6093/1‑149 (MQ=255)                                                                                                                                                   aGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGAc  <  3:173810/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                       CAGCCTGCCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGAC  >  NC_002947/5917497‑5917791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑