Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,917,649 T→C 7.5% L236P (CTG→CCG) ‡ ygiF → thiamine triphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,917,6490TC 7.5% 149.0 / 2.8 53L236P (CTG→CCG) ‡ygiFthiamine triphosphatase
Reads supporting (aligned to +/- strand):  ref base T (23/26);  new base C (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

CTGCCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGACC  >  NC_002947/5917501‑5917792
                                                                                                                                                    |                                                                                                                                                 
cTGCCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAgctgct                                                                                                                                                   >  2:6265/1‑149 (MQ=255)
     aCTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGCCGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTggg                                                                                                                                                >  4:97258/1‑147 (MQ=255)
     aCTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGgca                                                                                                                                              >  3:13721/1‑149 (MQ=255)
          tGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCagcagc                                                                                                                                         <  2:84244/149‑1 (MQ=255)
              gTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCTGCTGCCCAGCAGctgccagg                                                                                                                                     <  7:61470/149‑2 (MQ=255)
              gTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGcagccagc                                                                                                                                     >  8:211123/1‑149 (MQ=255)
                      tCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCAGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGcc                                                                                                                             >  8:261104/1‑149 (MQ=255)
                               cTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTAc                                                                                                                    <  1:47131/149‑1 (MQ=255)
                                 gAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCg                                                                                                                  <  3:127070/149‑1 (MQ=255)
                                        gTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTgg                                                                                                                                                 <  4:253090/111‑1 (MQ=255)
                                        gTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTgg                                                                                                                                                 >  3:253090/1‑111 (MQ=255)
                                             cGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCa                                                                                                      <  1:21676/149‑1 (MQ=255)
                                             cGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCa                                                                                                      >  8:279421/1‑149 (MQ=255)
                                              gCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCAc                                                                                                     >  1:245494/1‑149 (MQ=255)
                                                         ccGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCt                                                                                        >  4:9119/1‑149 (MQ=255)
                                                          cgtaCAGCTACGAGCTTGACCTGCCGCTCTCCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGGCAGCGCCTGGGCGATCAGTACCGCCACAACGGCCTCTGG‑‑CGCCTGCTc                                                                                       <  2:229832/146‑1 (MQ=255)
                                                          cGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTc                                                                                       <  7:261104/149‑1 (MQ=255)
                                                             aCAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCCGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAg                                                                                    <  1:6265/149‑1 (MQ=255)
                                                                 cTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCTCGACTGCTCCAGGa                                                                                  >  1:114442/1‑149 (MQ=255)
                                                                  tACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTg                                                                               >  6:106440/1‑149 (MQ=255)
                                                                  tACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTg                                                                               >  6:164411/1‑149 (MQ=255)
                                                                    cGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGTCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTg                                                                               <  7:211123/147‑1 (MQ=255)
                                                                    cGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTggg                                                                             <  2:54002/149‑1 (MQ=255)
                                                                       gCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCAGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTc                                                                           >  8:101118/1‑148 (MQ=255)
                                                                        cTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTc                                                                           >  5:251872/1‑147 (MQ=255)
                                                                        cTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGa                                                                         <  8:74290/149‑1 (MQ=255)
                                                                           gACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAg                                                                        <  2:245494/147‑1 (MQ=255)
                                                                            aCCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGc                                                                     >  1:66990/1‑149 (MQ=255)
                                                                             ccTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGa                                                                         >  5:215172/1‑144 (MQ=255)
                                                                             ccTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGa                                                                         <  6:215172/144‑1 (MQ=255)
                                                                             ccTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGcc                                                                    <  5:106440/149‑1 (MQ=255)
                                                                                 ccGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTg                                                                  >  8:303678/1‑147 (MQ=255)
                                                                                 ccGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTg                                                                  <  7:303678/147‑1 (MQ=255)
                                                                                    cacaCCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTgagcga                                                             <  2:66990/149‑1 (MQ=255)
                                                                                     acacCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTgagccag                                                            >  8:217884/1‑149 (MQ=255)
                                                                                       acCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCt                                                          >  5:224958/1‑149 (MQ=255)
                                                                                       acCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCt                                                          >  7:13144/1‑149 (MQ=255)
                                                                                                  cGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCa                                               >  4:173810/1‑149 (MQ=255)
                                                                                                   gCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGCGCGCAGCTCGCTCag                                              <  3:13717/149‑2 (MQ=255)
                                                                                                     cGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCg                                            <  3:9119/149‑1 (MQ=255)
                                                                                                             cccTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTg                                    <  7:101118/149‑1 (MQ=255)
                                                                                                              ccTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTgg                                   >  1:16832/1‑149 (MQ=255)
                                                                                                               cTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTggg                                  <  5:164411/149‑1 (MQ=255)
                                                                                                               cTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTggg                                  <  7:280867/149‑1 (MQ=255)
                                                                                                               cTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTggg                                  >  8:280867/1‑149 (MQ=255)
                                                                                                                     gacgCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGgc                            <  6:251872/149‑1 (MQ=255)
                                                                                                                       cgCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCGCTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGgcag                          <  4:13721/149‑1 (MQ=255)
                                                                                                                       cgCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGgcag                          <  6:224958/149‑1 (MQ=255)
                                                                                                                                         cTGGCTGCTGCCCAGCAGCTGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCAccc        >  5:302000/1‑149 (MQ=255)
                                                                                                                                         cTGGCTGCTGCCCAGCAGCTGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCAccc        <  6:302000/149‑1 (MQ=255)
                                                                                                                                         cTGGCTGCTGCCCAGCAGCTGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCAccc        >  6:320170/1‑149 (MQ=255)
                                                                                                                                            gCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCcgcg     <  8:13144/149‑1 (MQ=255)
                                                                                                                                            gCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCcgcg     >  8:6093/1‑149 (MQ=255)
                                                                                                                                              aGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGAc   <  3:173810/149‑1 (MQ=255)
                                                                                                                                               gctgctGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGAcc  >  8:27842/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTGCCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGG‑‑CGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGACC  >  NC_002947/5917501‑5917792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑