Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,959,996	A→T	8.4%	noncoding (73/103 nt)	PP_mr63 ←	rnk_pseudo ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,959,996	0	A	T	8.4%	179.2 / 4.2	67	noncoding (73/103 nt)	PP_mr63	rnk_pseudo ncRNA
Reads supporting (aligned to +/- strand):  ref base A (29/32);  new base T (3/3);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTC  >  NC_002947/5959850‑5960141                                                                                                                                                          |                                                                                                                                                  gTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑Agg                                                                                                                                                 <  4:9866/149‑1 (MQ=255) gTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑Agg                                                                                                                                                 <  5:154837/149‑1 (MQ=255)   aCTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGGTTTCAGTGTGCAAATTAAGCAAAGCCCCGA‑‑‑CCA‑‑AGGGc                                                                                                                                               >  6:228658/1‑149 (MQ=255)    ctctCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGc                                                                                                                                               <  6:18013/148‑1 (MQ=255)     tctcGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCgg                                                                                                                                             >  6:21160/1‑149 (MQ=255)     tctcGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCgg                                                                                                                                             >  2:210196/1‑149 (MQ=255)     tctcGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCgg                                                                                                                                             >  1:57581/1‑149 (MQ=255)       tcgtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCgggg                                                                                                                                           >  4:283742/1‑149 (MQ=255)          tcgAGGCTTTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGTTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGttt                                                                                                                                        <  5:213644/149‑1 (MQ=255)          tcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGttt                                                                                                                                        <  4:179591/149‑1 (MQ=255)          tcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGttt                                                                                                                                        <  4:122884/149‑1 (MQ=255)          tcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGttt                                                                                                                                        >  1:258532/1‑149 (MQ=255)           cgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTg                                                                                                                                       <  1:182807/149‑1 (MQ=255)            gAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCT‑‑TGGGCGGGGttttg                                                                                                                                      <  2:178965/149‑3 (MQ=255)                   tCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCt                                                                                                                               <  3:283742/149‑1 (MQ=255)                      aTCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTg                                                                                                                            <  2:170537/149‑1 (MQ=255)                      aTCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTg                                                                                                                            <  5:13425/149‑1 (MQ=255)                      aTCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTg                                                                                                                            <  5:21160/149‑1 (MQ=255)                         aGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGc                                                                                                                          <  7:29168/148‑1 (MQ=255)                           aCGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAAGGGCTTGGTGATCAGGATCCAGAAAGCATCCTTTGAACGGGAAGTTTTCATTTTGAAATAAAAGAAAAACCCCGC‑‑‑CCA‑‑TGGCGGGGGTTTGAGAAGGCTTTgcgt                                                                                                                         >  1:279150/1‑145 (MQ=255)                           aCGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTc                                                                                                                       <  7:164512/149‑1 (MQ=255)                            cccTCCATCCGGTGTAAGTCCAATCGGGTGATGCAGTTGGCAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGt                                                                                                                        <  2:279150/146‑1 (MQ=255)                                 cAGACACTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGaa                                                                                                                                   <  2:298596/131‑1 (MQ=255)                                 cAGACACTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGaa                                                                                                                                   >  1:298596/1‑131 (MQ=255)                                    aCGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCa                                                                                                              <  1:210196/149‑1 (MQ=255)                                      gCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAgg                                                                                                                                 >  2:177499/1‑128 (MQ=255)                                      gCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAgg                                                                                                                                 <  1:177499/128‑1 (MQ=255)                                      gCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAg                                                                                                             >  4:288444/1‑148 (MQ=255)                                         gTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAAcc                                                                                                         <  5:228658/149‑1 (MQ=255)                                              tCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCATTGTAGGAGGGCGTGGTTTGAGAAGTATTTGGCGTCAACGACGCAGAAcc                                                                                                         >  1:111096/1‑149 (MQ=255)                                               ccAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGa                                                                                                      >  3:297402/1‑146 (MQ=255)                                               ccAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGa                                                                                                      <  4:297402/146‑1 (MQ=255)                                                cAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCCCCGACGCAGAACCCGacac                                                                                                   >  8:176143/1‑148 (MQ=255)                                                 aaTCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGaa                                                                                                                                   <  1:122673/115‑1 (MQ=255)                                                 aaTCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGaa                                                                                                                                   >  2:122673/1‑115 (MQ=255)                                                  aTCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTAc                                                                                                >  5:71796/1‑149 (MQ=255)                                                        tGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGATGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGc                                                                                          >  5:282429/1‑149 (MQ=255)                                                             gATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCggg                                                                                      <  6:71796/149‑1 (MQ=255)                                                                    gAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGCCACTACGCCAGCCGGGCAAATag                                                                               >  4:93800/1‑148 (MQ=255)                                                                          cTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAg                                                                          >  3:137983/1‑149 (MQ=255)                                                                          cTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTATCAATGTGCAAATAAACCAAAACCCCGC‑‑‑CCA‑‑AGGGCGCGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAg                                                                          >  5:286276/1‑149 (MQ=255)                                                                                gCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTg                                                                    >  3:45434/1‑149 (MQ=255)                                                                                gCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGAAACTACCACAGCCGGGCAAATACTCAAGCCCCTg                                                                    >  6:177771/1‑149 (MQ=255)                                                                                    aTGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCt                                                                <  3:93800/149‑1 (MQ=255)                                                                                       aTCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑ACA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAg                                                             >  4:144250/1‑149 (MQ=255)                                                                                               aGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCt                                                      <  3:144250/148‑1 (MQ=255)                                                                                                  cTCCTTTGAACAGGCTGTTATCAATGTGCAATTAAAGCAAAACCCCGC‑‑‑CCA‑‑TGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGTCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGc                                                  <  6:286276/149‑1 (MQ=255)                                                                                                  cTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGc                                                  <  4:137983/149‑1 (MQ=255)                                                                                                            cAGGCAGTTTTCAATGTGAAAATACAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCgcgc                                        <  8:262272/149‑1 (MQ=255)                                                                                                              ggCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCaa                                      <  2:258532/149‑1 (MQ=255)                                                                                                                           gtgCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcg                         >  7:25645/1‑149 (MQ=255)                                                                                                                            tgCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgc                        >  3:178482/1‑149 (MQ=255)                                                                                                                               aaaTAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCgg                      >  7:104987/1‑148 (MQ=255)                                                                                                                               aaaTAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGc                     <  8:25645/149‑1 (MQ=255)                                                                                                                                aaTAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCgg                      <  2:98465/147‑1 (MQ=255)                                                                                                                                aaTAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCgg                      >  1:98465/1‑147 (MQ=255)                                                                                                                                  tAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTc                                            <  4:111058/123‑1 (MQ=255)                                                                                                                                  tAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTc                                            >  3:111058/1‑123 (MQ=255)                                                                                                                                   aaaCCAAAACCCCGC‑‑‑CCA‑‑AGCGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGAt                 <  5:99831/149‑1 (MQ=255)                                                                                                                                      ctcaaaCCCCGC‑‑‑CCT‑‑TGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTg              >  7:117997/4‑149 (MQ=255)                                                                                                                                       cAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGc             >  4:243841/1‑149 (MQ=255)                                                                                                                                           aCCCCGC‑‑‑CCT‑‑TGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGgc         >  8:238032/1‑149 (MQ=255)                                                                                                                                             cccGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTgg                               >  5:236423/1‑125 (MQ=255)                                                                                                                                             cccGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTgg                               <  6:236423/125‑1 (MQ=255)                                                                                                                                               cGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCg     >  1:75144/1‑149 (MQ=255)                                                                                                                                                     ccA‑‑TGGGCGGGTTTTGATAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTc  <  8:104987/149‑1 (MQ=255)                                                                                                                                                     ccA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTc  <  7:176143/149‑1 (MQ=255)                                                                                                                                                          |                                                                                                                                                  GTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGA‑GGATGAGGGAA‑GGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGC‑‑‑CCA‑‑AGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTC  >  NC_002947/5959850‑5960141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑