Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,962,273 A→C 6.0% D390A (GAC→GCC)  lysA‑II → diaminopimelate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,962,2730AC 6.0% 202.9 / 4.2 67D390A (GAC→GCC) lysA‑IIdiaminopimelate decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (32/31);  new base C (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTCAG  >  NC_002947/5962130‑5962421
                                                                                                                                               |                                                                                                                                                     
cGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACg                                                                                                                                                     <  3:65661/146‑1 (MQ=255)
     cTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAg                                                                                                                                             <  6:131081/149‑1 (MQ=255)
      tGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGCCGTCGACCAg                                                                                                                                             >  4:148316/1‑148 (MQ=255)
        gCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGGCCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCg                                                                                                                                          >  1:234763/1‑149 (MQ=255)
        gCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGCCGTCGACCAgga                                                                                                                                           <  4:88257/148‑2 (MQ=255)
        gCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGCCGTCGACCAgga                                                                                                                                           <  2:98186/148‑2 (MQ=255)
        gCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGc                                                                                                                                           <  2:3616/148‑1 (MQ=255)
        gCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGc                                                                                                                                           <  3:287543/148‑1 (MQ=255)
               ccTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGaa                                                                                                                                    <  1:265771/148‑1 (MQ=255)
                cTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAg                                                                                                                                   <  3:294346/148‑1 (MQ=255)
                 tCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTa                                                                                                                                 >  4:276970/1‑149 (MQ=255)
                     cAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTAcgcc                                                                                                                             >  2:207191/1‑149 (MQ=255)
                           ccGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTAcgccgccgc                                                                                                                        >  4:32049/1‑148 (MQ=255)
                           ccGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTAcgccgccgc                                                                                                                        >  2:187508/1‑148 (MQ=255)
                            cgcgTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTAcgccgccgc                                                                                                                        >  2:46250/1‑147 (MQ=255)
                            cgcgTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTAcgccgccgc                                                                                                                        >  7:71404/1‑147 (MQ=255)
                                 ccTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCg                                                                                                                 >  6:9254/1‑149 (MQ=255)
                                   tCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACAACCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTc                                                                                                               >  6:15175/1‑149 (MQ=255)
                                     aaCCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTc                                                                                                               <  1:207191/147‑1 (MQ=255)
                                     aaCCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGATCGAAGTACGCCGCCGCGAGACCGTCGc                                                                                                             <  1:187508/149‑1 (MQ=255)
                                           gCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGTCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGCACt                                                                                                       >  1:139658/1‑149 (MQ=255)
                                             cGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGTCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTg                                                                                                      >  5:81060/1‑148 (MQ=255)
                                             cGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGt                                                                                                     <  3:32049/149‑1 (MQ=255)
                                             cGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCAGCGAGACCGTCGCCGAACTg                                                                                                      >  3:137107/1‑148 (MQ=255)
                                               aaGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTAt                                                                                                   <  1:46250/149‑1 (MQ=255)
                                                aGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATg                                                                                                  >  6:118125/1‑149 (MQ=255)
                                                   tGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTg                                                                                               <  2:200828/149‑1 (MQ=255)
                                                       ctgctgGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCACTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCg                                                                                            >  6:141247/1‑148 (MQ=255)
                                                        tgctgGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTg                                                                                               <  5:210602/144‑1 (MQ=255)
                                                        tgctgGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTg                                                                                               >  6:210602/1‑144 (MQ=255)
                                                         gctgGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGa                                                                                           >  5:68306/1‑147 (MQ=255)
                                                         gctgGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGa                                                                                           >  5:195447/1‑147 (MQ=255)
                                                          ctgGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGaa                                                                                          <  5:9254/147‑1 (MQ=255)
                                                                      tCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCg                                                                             >  8:86422/1‑147 (MQ=255)
                                                                      tCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGa                                                                           <  8:71404/149‑1 (MQ=255)
                                                                        cgcgGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGt                                                                         <  2:234763/149‑1 (MQ=255)
                                                                                    tGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCAtgctg                                                             <  6:68306/149‑1 (MQ=255)
                                                                                             cATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGttt                                                     >  2:88984/1‑148 (MQ=255)
                                                                                               tGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTa                                                   <  1:45189/149‑1 (MQ=255)
                                                                                               tGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTAc                                                  >  3:194098/1‑149 (MQ=255)
                                                                                                 aGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTAcc                                                 <  5:118125/148‑1 (MQ=255)
                                                                                                    tCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTAcc                                                 >  2:165083/1‑145 (MQ=255)
                                                                                                      cAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGCCCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATg                                           >  8:114378/1‑149 (MQ=255)
                                                                                                      cAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATg                                           >  3:201898/1‑149 (MQ=255)
                                                                                                       aaCTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGc                                          <  4:201898/149‑1 (MQ=255)
                                                                                                          tACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTgg                                                                                              >  1:264845/1‑95 (MQ=255)
                                                                                                          tACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTgg                                                                                              <  2:264845/95‑1 (MQ=255)
                                                                                                            caacaCCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtg                                       <  5:15175/147‑1 (MQ=255)
                                                                                                             aacaCCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggc                                    >  6:332536/1‑149 (MQ=255)
                                                                                                              acacCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggct                                   >  4:35960/1‑149 (MQ=255)
                                                                                                                acCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggctgg                                 >  5:296114/1‑149 (MQ=255)
                                                                                                                 cccGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggctggg                                <  4:194098/149‑1 (MQ=255)
                                                                                                                  ccGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggctgg                                 <  8:98458/147‑1 (MQ=255)
                                                                                                                  ccGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggctgg                                 >  7:98458/1‑147 (MQ=255)
                                                                                                                       gCCGTTGCGCTTAAATCCTGGTCTACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGa                          <  4:148543/149‑1 (MQ=255)
                                                                                                                           ttGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTc                      <  3:276970/149‑1 (MQ=255)
                                                                                                                              cgcTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCAt                    <  4:137107/148‑1 (MQ=255)
                                                                                                                              cgcTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATg                   >  4:767/1‑149 (MQ=255)
                                                                                                                              cgcTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATg                   <  1:88984/149‑1 (MQ=255)
                                                                                                                                  gAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTcc               >  1:85413/1‑149 (MQ=255)
                                                                                                                                    aaTCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTg             <  5:332536/149‑1 (MQ=255)
                                                                                                                                    aaTCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTg             >  5:242150/1‑149 (MQ=255)
                                                                                                                                    aaTCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTg             <  6:242150/149‑1 (MQ=255)
                                                                                                                                      tCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAAc                            >  2:328952/1‑132 (MQ=255)
                                                                                                                                      tCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAAc                            <  1:328952/132‑1 (MQ=255)
                                                                                                                                          ggTCGCCGTCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTg       >  4:302251/1‑149 (MQ=255)
                                                                                                                                              gACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTca   <  3:35960/149‑1 (MQ=255)
                                                                                                                                               aCGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTcag  <  3:767/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
CGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAG‑CCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTCAG  >  NC_002947/5962130‑5962421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑